**5.2 In-vivo test**

In-vivo test, testing performed inside animal tissue. There are two methods for Vivo testing, embedding an implantable antenna inside donor animals and surgically implanting an antenna inside a live animal. In [64, 89] dual bands MICS (402–405 MHz) and ISM (2.4–2.48GHz) tested in vivo. A vivo testing protocol must be developed before the experimental investigation. Pre-surgical preparation, anesthesia, etc. should be needed. In [89], two antennas were implanted in three different rats. Due to surgical procedure variation, affect exhibited on return loss frequency response. Dielectric properties of live tissue generally depend on frequency, age, temperature, sex, etc. parameters. In [90–93] in vivo testing was performed to explore the effect of live tissues on antenna performance. In [91] biocompatible capsule device was implanted inside a live pig body for temperature monitoring. Two circular polarized antennas were tested in rat muscle in [93].
