**5.1 In-vitro test**

The meaning of In-Vitro is an outside living organism, this test is relatively easy and practically implementable because testing exists inside the phantom. Phantom is a container (cube or box) with liquid or gel material, it consists of the electrical properties of biological tissue. Fabricated prototype inserted in tissue phantom and measured. Phantoms are generally prepared with the help of deionized water,

*Microwave Antennas Suggested for Biomedical Implantation DOI: http://dx.doi.org/10.5772/intechopen.101060*

sugar, salt, etc. If sugar concentration is increased, the permittivity of tissue significantly decreases and conductivity slightly increases and if salt concentration increased, it results in permittivity of tissue decreases and conductivity significantly increases. The mixture must be properly heated and stirred to avoid air bubble formation and poured inside the phantom. In [88] Measurement of liquids electrical properties (εr and μ) was conducted with a dielectric probe kit or open-ended coaxial cable. Generally, reflection coefficient, path loss, communication link, and polarization factor, etc. measured in vitro vest, as observed in the literature. Generally, prototype antennas are connected with a network analyzer through a coaxial cable, inserted in a tissue phantom, and measured.
