**6.2 Sample collection and preservation**

Freshly cut Femur bone samples from Goat Male were collected. All muscles and soft tissue of bone should be removed. Samples to be washed with water and kept in hydrated condition. The weight of the Goat male femur bone was 125gm and length was 18 cm, While the weight of one buffalo male or female femur bone was 1100 gm and the length was 35–40 cm. The diameter of the long bone middle section of female buffalo femur bone was seen to be greater than male buffalo femur bone. But the length of male buffalo femur was found greater than female buffalo femur.

### **Figure 1.** *Test specimen, fresh femur bone of goat male (Capra aegagrushircus).*

The sample was preserved in distill water at 24 degrees Celsius. Samples were tested within 5 days.
