*4.5.2 TEM study of blood serum of bovine (cattle) mixed with ZnO*

TEM images are observed using TEM (JEOL-100CX) as shown in the **Figure 13** after mixing 1ml blood serum of bovine with 1 μl of ZnO using micropipette. The irregular black spots of serum proteins are observed [16].

*Device Diagnosing Health of Bovine DOI: http://dx.doi.org/10.5772/intechopen.99286*

In TEM study, the irregular black spots of about 30-40nm are serum proteins. The average size of serum albumin is 36 nm. Bubble like structure are the aggregated lipoproteins. It has been observed that mixing of 1μl ZnO with 1ml blood serum cause an increase in order of the biological parameters viz. BUN, creatinine, SGPT and SGOT and electrical parameter (resistance) which will help to analyse the variation of biological parameters of blood serum, after the incorporation of ZnO nanoparticle in it [17].

This study also tells us that the protein structure still exist in the images observed from TEM of blood serum of bovine mixed with ZnO same as that of the images observed from TEM of only blood serum of animal .
