**6.3 Sample cutting and preparation**

Yuri 4 inch Cutting Wheel Diamond cutter was used for cutting sample as shown in **Figure 2**. At the time of cutting of sample with a diamond cutter, bone sample reduces its strength and minerals because of high heat formation due to friction at the cut. It finally causes error in results. For good results samples were prepared by using saline water as a lubricant to release heat at the interface of diamond cutter and bone.
