**2. Material and methods**

### **2.1 Sample collection**

Sampling strategy is comprehensively discussed in **Table 1**. After collection, samples were transported in icebox to University of Veterinary and Animal Sciences, Lahore where these were stored at −4°C till further processing.

### **2.2 Sample processing**

### *2.2.1 Processing of milk samples through rapid test kit*

Rapid test kit detects AFM1 to the limit of 0.5 ng/ml-ppb. The kit was used according to manufacturer instructions. Briefly, 200 μL milk samples were pipetted into reagent microwell and after mixing, these samples were incubated at room *An Assessment and Control of AFM1 in Milk and Main Dairy Products in Lahore, Pakistan DOI: http://dx.doi.org/10.5772/intechopen.99184*


### **Table 1***.*

*Sampling strategy.*

temperature for 2 minutes. Then dipstick was inserted in each sample and incubated for another 5 minutes at the same temperature. After that dipstick was taken out from each sample and samples were interpreted. There would be two lines i.e. test line (T) and control line (C) on dipstick. If T > = C then sample was considered negative while if T > C or there was no test line then it was considered as positive.
