**3.1 Experiments for investigation of antimicrobial potential of probiotics** *In vitro*

Included in this section are the *In vitro* antagonism methods on agar plates and liquid coculture for checking probiotic antimicrobial activity with their strengths and drawbacks. In antagonism on agar plate's methods, probiotic and indicator microorganism is introduced in the same plate. The difference is the sequence and manner of the inoculation of either indicator pathogen or probiotic microorganisms. After incubation, the diameter zone of inhibition which is the clear zones around the inoculated area, is then read in millimeters or reported or arbitrary units (AU) (**Figures 2** and **3**).
