**2.6 Other secreted molecules**

The cholix toxin (ChxA) is a eukaryotic elongation factor-2 specific ADPrybosyl transferase that induces cell death [133]. ChxA is produced by many *V. cholerae* strains [134, 135].

Accessory cholera enterotoxin (Ace - VC1459) and zonula occludens toxin (Zot - VC1458) are accessory toxins that are both encoded near the CT genes on the CTXφ phage [136, 137]. Zot leads to the disruption of the tight junctions between intestinal epithelial cells, an important structure in the intestinal permeability [138, 139]. It is translocated and anchored in the outer membrane and has two functional domains. The N-terminal domain is important for CTXφ phage's morphogenesis and the C-terminal domain is cleaved by proteases. Once released into the intestinal lumen, the C-terminal domain acts as a toxin [139, 140]. Thus, Zot does not employ a conventional secretion system for its release into the extracellular milieu. Regarding Ace, it leads to fluid secretion in rabbit ileal loop model by unbalancing calcium secretion and the secretion mechanism has not been determined yet [138].
