*Bacillus megaterium* Biodegradation Glyphosate

*Nibal Khaleel Mousa, Abdul-Jabbar Ali and Maha Hussein*

### **Abstract**

The *Bacillus megaterium* ability was evaluated in this paper to degrade the Glyphosate. organophosphorus pesticides, The bacteria re-cultured that isolated from other researches of Baghdad soils and morphological identification and biochemical tests besides by selectivity media*.* The (5 and 25) ppm showed the highest growth results were within two days to two months on mineral salt media. The highest glyphosate degradation ratio % were (70) % per 25 ppm/two months. Incubation period Increasing led to highest glyphosate degradation ratio% at (25) ppm led to conclusion that bacteria digestive the pesticides as carbon and nitrogen sources and will be well harvest it form contaminated areas.

**Keywords:** bacterium, bioremediation, glyphosate, HPLC

#### **1. Introduction**

Glyphosate is an organophosphate which is a heterogeneous compound, utilized on the grasses leaves and broadleaf plants due its non-selectivity pesticides. In the seventies was the first registration in the United State by Monsanto (Roundup) [1]. Glyphosate stops a shikimic acid enzyme pathway which is essential for some microorganisms and plants. In trace analysis, Glyphosate is a difficult herbicide, has a good water solubility that causes difficulty in determination its physical and chemical properties [2].

The microorganism ability to eliminate pollutants is one of the bioremediation methods [3]. Bioremediation is applied as an auxiliary strategy due to its inoffensive to ecology, economic worth, reduce environmental poison, and validation [4–8]. Metabolic is one of the microorganism degradation methods across pesticides in soils, also catabolism strategies and the enzymes of co-metabolism [9]. Organic-pesticides fate in the ecology system can be marked by utilizing biodegradation as a major agent. The study purpose is to achieve and inspect the domestic bacterial separated on broken down different glyphosate concentrations that can be remaindered in soil and detect residues concentration from bacteria digestive via HPLC after extraction.

### **2. Material and methods**

#### **2.1 Materials**

Chemosate. The trade name of "Glyphosate", bought from the local market. Materials were available and supply in Remediation Pollutants Center. The Mineral

