**2.4 Metabolite analysis**

Each ethyl acetate extraction was analyzed by HPLC condition, **Table 3** [14], calculates the final concentration [15] of glyphosate used Eq. (2):

$$\text{Post.con} = \text{Asa} \propto \text{Cs} \land \text{As} \propto \text{Csa} \tag{2}$$

Pest.con = pesticide concentration in sample (mg/L) Asa = sample peak area Csa = sample concentration, mg/L As = standard. peak.area Cs = standard concentration, mg/L
