**2.3 Glyphosate degradation ratio via** *Bacillus megaterium*

The degradation ratio % investigated for (1-2) months, extracted through added equal volume each ethyl acetate and MSM which utilized as a reagent, for twice time, centrifuged at 3000 rpm/10 minutes, filtered then anhydrous sodium sulfate utilized as dry factor followed glass-fiber paper (Whatman GF/B) [10]. In Eq. (1), measured the degradation ratio:

$$P = \left(1 - \frac{C1}{C0}\right) \times 100\% \tag{1}$$

P = the rate of degradation of Glyphosate,


**Table 3.** *HPLC conditions.*

> C1 = Glyphosate dose in sample. C0 = control [13].
