**2.2 Re-growth and identification of** *Bacillus megaterium*

*Bacillus megaterium* was growth and kept in incubation of our laboratory from other studies [3] and to re-identification by selective media, was by Sperber's Medium [10, 11], **Table 2**, By the spectrophotometer OD 600, then the hydrolysis capacity measured for different period (2,5,7,14,21,30,60) days [12].
