**2. Experimental details**

#### **2.1 Materials and methods**

AgNO3 (Silver nitrate, 99.99%) was procured from Sigma Aldrich. *Raphanussativus* leaves were got from the market. Lanthanum complexes Eu (TTFA)3 and Sm(TTFA)3 were obtained from Soltech BioScience and Rare Earth products Inc., USA.

## **2.2 Preparation of Raphanussativus leaf extract**

First Raphanussativus leaves were wiped using distilled water. 10 grams of *Raphanussativus* leaves have taken into 100 ml of distilled water and heated for 5 minutes and grinded in a pestle. The mixture solution was filtered with Whatman no. 1 filter twice to get rid of granules and refrigerated at 4°C.

### **2.3 Synthesis of Ag NPs using Raphanussativus leaf extract**

1.5 ml of freshly prepared leaf extract was poured into 30 ml of silver nitrate (1 mM) aqueous solution with stirring at room temperature. Then, the solution changed from transparent to ash color in 5 minutes, which represents the formation *Green Synthesis of Metal Nanostructures and Its Nonlinear Optical Properties DOI: http://dx.doi.org/10.5772/intechopen.99449*

**Figure 1.** *Synthesis of Ag nanoparticles.*

of silver nanoparticles. The nanoparticles were stable for few months. Preparation process can be shown in **Figure 1**.
