**2. Materials and methods**

In this study, probiotic juices containing 2 × 109 cfu *Bacillus coagulans* were prepared. 60 male runner athletes with an age range of 18 to 20 years and an average weight of 68 to 72 kg were randomly divided into two groups of 30 people. The criterion for participating in the study of complete physical health and was nonsmoking. During the 12 weeks of the study, the experimental group received a glass of probiotic juice containing *Bacillus coagulans* daily, and the control group received fruit juice Without supplemental.
