**Abstract**

Probiotics exert beneficial effects on their host health by creating microbial balance in the digestive system. The role of some probiotic strains in strengthening the immune system and reducing the risk of diseases, especially respiratory infections, has been proven in previous studies. Aim: The aim of this study was to evaluate the effect of probiotic supplementation containing *Bacillus coagulans* on the Runner athletes immune system. In this study, the effect of *Bacillus coagulans* probiotic on immunoglobulins A, M and monocytes count 60 male athlete sprints Evaluates that which were randomly divided into two groups of 30.For 3 months, the experimental group received a daily glass of probiotic juice containing 109 cfu / ml containing probiotic supplement and the control group received plain and no supplemental juice. During the study period, once every 2 weeks, One day after exercise (running 200 meters), blood samples were taken from all participants Then In the collected samples, IgA, IgM and lymphocytes were evaluated. Consumption of probiotic juice containing 2 × 109 f cfu/ml *Bacillus coagulans* probiotic supplement showed a significant difference in the amount of IgA, IgM and Lymphocyte between the experimental group and the control group. The results of this study showed that the consumption of juice containing probiotic supplement *Bacillus coagulans* can increase the level of immune factors IgM, IgA, lymphocytes and prevent the occurrence of diseases, especially respiratory infections, by improving the function of the immune system.

**Keywords:** IgA, IgM, probiotic juice, immune system, lymphocytes, athletes
