**1. Introduction**

Mitochondria possess a small spherical genome, mtDNA, which encodes for the 13 important subunits of the electron transport chain and ATP synthase together with 22 tRNAs and 2 rRNAs necessary for mitochondrial protein synthesis [1, 2]. Mitochondrial DNA presents several characteristics which have the potential to be valuable for forensic studies, especially attendant to the absence of recombination, to a large copy number, and to matrilineal inheritance. Mitochondrial DNA typing founded on sequences of the control region otherwise filled genomic sequence is used to examine a variation of forensic mtDNA

profiling methods used for human proof of identity and present their use in the chief cases of human identification from non-human [3–5]. Mitochondrial markers that are used for species identification are as follows: cytb gene, cytochrome c oxidase subunit I gene, 12S and 16S rRNA segment, and control region in wildlife [6–8]. A short fragment of the 12S rDNA was employed for DNA amplification leading to species identification. The mitochondrial DNA 16S rRNA gene is an advanced genetic marker for animal genetic diversity. Polymorphism sites, nucleotide variation, and haplotype variety were determined using whole sequences of the mitochondrial DNA 16S rDNA gene [9, 10]. Animal mitochondrial DNA (mtDNA) is commonly described as a small, circular molecule that is conserved in size, gene content, and organization [11]. The aim of this study is to design valuable species-specific-PCR tool to discriminate blood of humans from non-human using a species-specific primer design.
