**2.3 Primer design**

The gene selected for this study is the mitochondrial 16S rRNA gene. The NCBI data base was used to recover the sequences chosen for a primer design. The sequence ID of *Homo sapiens* (NC\_012920.1); sequence ID of *Ovis aries* (NC\_001941.1); sequence ID of *Capra hircus* (NC\_005044.2); sequence ID of *Bos taurus* (NC\_006853.1). Primer 3 software [12] was used to design the specific primer using the sequence of above-mentioned sequence IDs. The generated primers were as follows: Homo 16S-F: GCCTGGTGATAGCTGGTTGT, Homo 16S-R: ATCATTTACGGGGGAAGGCG (1200 bp); Ovis 16S-F: AGGCCTAAAAGCAGCCATCA, Ovis 16S-R: GCCCTTTTCTAGGGCAGGTT (1060 bp); Capra 16S-F: GCCTGGTGATAGCTGGTTGT, Capra 16S-R: TCACCCCAACCAAAACTGCT (820 bp); and Bos 16S-F: CTAAGCAGCCCGAAACCAGA, Bos 16S-R: GGGCAGGGTTTTGTGTTGTC (1300 bp).
