**6. The advantages and limitations in tissue culture technique**

Plant tissue culture technique permits the growing plants in test tube or closed container in vitro under controlled environment. This technique is devoted to solve two problems: 1) To keep the plant cells free from microbes. 2) To grow the desired plants by providing suitable nutrient medium and other environmental conditions. Several advantages of plant tissue culture techniques are as following:

	- ix.Seedless propagation Some plant seeds are difficult to germinate, tissue culture enables plant propagation without the need of seeds.
