ii.Cytological studies

Feulgen staining can be used to stain nuclei of the somaclones to measure DNA contents by using cytophotometer. Number of chromosomes can also be measured using the same technique on meristematic tissues such as root tip and shoot tip.

iii.Gel electrophoresis

This technique can be used to detect variation among somaclones in terms of the concentration of protein, pigments and amino acids through the observation of amplification pattern.

iv.Disease resistance trait

Pathogen or toxin that is responsible for the disease resistance can be used to select disease resistance clones.

v.Herbicide resistance trait

Plantlets that are grown on culture medium containing the particular herbicide possess the herbicide resistance trait.

vi.Stress tolerance trait

Detection for stress tolerance trait has been done on tobacco cell lines for high salt tolerance, and drought tolerance in tomato.
