**13. Autologous whole blood induced hemorrhage model**

This model is widely used to understand hemorrhagic stroke and mimic a condition similar to that in humans. This model animal will be anaesthetized using an intraperitoneal injection of pentobarbital (50 mg/kg). The animal will be placed on the stereotactic frame, and under aseptic condition, a scalp incision will be made. Further, a hole will be drilled 0.02 mm anterior to the coronal suture and 3 mm lateral to the midline. A 25-gauge needle will be introduced into the cerebral cortex region on the surface of the skull. The rat will receive 50 -μl of autologous whole bold for 5 min. After completing the infusion process, the needle will be kept at the same place for 3 min and later removed. The hole drilled in the skull will be closed using bone wax, and the scalp wound would be sutured [81].

Merits of autologous whole blood induced hemorrhage model [76].


Demerits of autologous whole blood induced hemorrhage model [76].

1.No significant neuronal loss found in the regions of the striatum.

*Experimental Animal Models of Cerebral Ischemic Reperfusion Injury DOI: http://dx.doi.org/10.5772/intechopen.97592*

