**12. Research envisaged using collagenase induced brain hemorrhage model**

N. Kawai et al. studied the effect of recombinant factor VIIa in a collagenaseinduced intracerebral hemorrhage model in rats. Early hematoma is the sign associated with neurological deterioration after intracerebral hemorrhage. Two hours after collagenase injection, there was blood accumulation in the striatum region and slowly extended to the thalamus region by 24 h. Thus, administration of recombinant factor VIIa immediately after collagenase injection would help in reducing the average hematoma volume and frequency of hematoma formation [77].

Arne Lauer et al., conducted a comparative study among direct thrombin inhibitor dabigatran etexilate (DE) over anticoagulant warfarin using collagenase induced Intracerebral Hemorrhage. The results found that intracerebral bleeding was severe during warfarin treatment and experimental data also confirms this as severe hematoma expansion. Treatment with dabigatran etexilate did not exaggerate the ongoing process of intracerebral bleeding neither increased hematoma growth. From the above results, we can confirm that cerebral hemorrhage occurring during DE treatment is minimal and harmless compared to warfarin [78].

P.P lema et al., have studied the dexamethasone's effect for treatment of intracerebral hemorrhage using a collagenase-induced intracerebral hematoma model in rats. After the successful induction of intracerebral hemorrhage, animals were evaluated for their neurological evaluation, followed by measurement of hematoma volume and necrotic tissue. Studies revealed that treatment with dexamethasone restored functional deficits, reduced hematoma volume, decreased filtration of neutrophils and astrocytes into hematoma and found to potentially active in the treatment of intracerebral hemorrhage [79].

Marc R. Del Bigio et al., have studied the effect of fucoidan on Collagenase induced intracerebral hemorrhage in rats. After the successful induction of intracerebral hemorrhage, animals were assessed for motor activity and forelimb functioning after six weeks and followed by hematoma evaluation. Animals treated with fucoidan for seven days successfully restored motor and cognitive activity but failed to prevent excess hematoma formation [80].
