**2.1 Background**

Mouse Model of Kidney Ischemia Reperfusion Injury: Warm ischemia and reperfusion injury models is the most widely used model of AKI, in rodents the renal blood flow is temporarily interrupted for a various time periods ranging from 20 to 45 minutes followed by varied amounts of reperfusion. In our laboratory we use a standard clamp time of 26 minutes followed by 24 hours of reperfusion. The IRI model of AKI can be further divided into bilateral, unilateral, and unilateral with simultaneous nephrectomy depending on the questions or therapeutic tested. In bilateral both renal pedicles are clamped, whereas unilateral with simultaneous nephrectomy can be utilized to reduce the variability that can be observed. However, in unilateral either left or right pedicle is clamped without disturbing the contralateral control kidney, ideally the unilateral IRI model is used for studies that have interest in evaluating progression to chronic kidney disease or fibrosis.
