**8. Research envisaged using the four-vessel occlusion model**

Ruchan ergun et al. studied the effect of propofol 2,6-diisopropylphenol following global cerebral ischemia–reperfusion injury using the four-vessel occlusion

#### *Experimental Animal Models of Cerebral Ischemic Reperfusion Injury DOI: http://dx.doi.org/10.5772/intechopen.97592*

method. In the present study, malondialdehyde was considered an important marker to estimate lipid peroxidation in ischemic tissue. The reviews concluded that propofol potentially inhibited neuronal death caused by four-vessel occlusion method-induced brain ischemia [65].

Levente Gellert et al. studied the effect of kynurenic acid in global forebrain ischemia insult buy evaluating the loss of CA1 (Hippocampal Cornu Ammonis) hippocampal neurons and long-term potentiation at Schaffer collateral- CA1 (Hippocampal Cornu Ammonis) synapses. The studies showed that kynurenic acid significantly prevented CA1 CA1 (Hippocampal Cornu Ammonis) hippocampal neuronal loss and preserved long-term potentiation expression. The main advantage of using kynurenic acid is that it was effective when used as pre-treatment and during reperfusion [66].

Hui Li et al. studied the Effect of Dehydroepiandrosterone in an animal model of transient severe forebrain ischemia. In this model, forebrain ischemia was induced by a modified four-vessel occlusion model where the occlusion was conducted for 10 min. The studies confirmed that treatment with Dehydroepiandrosterone protected hippocampal CA1 (Hippocampal Cornu Ammonis) area from injury and suggested that NMDA(N-methyl-D-aspartate) may not be the main contributor for hippocampal CA1 (Hippocampal Cornu Ammonis)neuronal cell injury [67].

D shivaraman et al., studied the effect of hemidesmus indicus on cerebral infarct ischemia–reperfusion injury by four-vessel occlusion method. The studies found that hemidesmus indicus significantly improved that neuromuscular, vestibulomotor, motor action, and decreased lipid peroxidation. Treatment also restored levels of dopamine and serotonin. The above results, it confirmed that hemisesmus indicus produced a neuroprotective effect in ischemic induced brain damage [68].
