Antioxidant and Oxidative Stress

*Betül Çalişkan and Ali Cengiz Çalişkan*

## **Abstract**

Antioxidants are compounds that eliminate oxidative stress in biological systems. Oxidative stress is caused by various radicals formed in the system as a result of oxygen entering the biological system. Structures with unpaired electron are either free radicals or radical ions. Antioxidants neutralize free radicals or radical ions due to the unpaired electron in their structure. The radical ions formed as a result of oxidation is removed from the system without damaging the biological system with the effect of antioxidants. There are many free radicals and radical ions. Among these radical groups are radical ions formed by oxygen which are important for biological systems. Antioxidants are responsible for the destruction of such radicals.

**Keywords:** antioxidant, oxidative stress, free radical, radical ions

## **1. Introduction**

## **1.1 Oxidative stress**

Oxidative stress is described as the disturbance of the balance between prooxidants (free radicals) and antioxidants. Oxidative stress occurs with the effect of free radicals and oxidants. Free radicals have unpaired electrons. Therefore, they create the oxidation process in the body. Oxidation causes an oxidative stress to occur. Oxidation processes and free radicals are constantly formed in the body. Oxidation is a process that can be harmful or beneficial. If the increase in free radicals is not balanced with antioxidants, the ground is prepared for the harmful process. It plays an important role in the development of diseases as it causes various damages. It creates DNA, lipid and protein damage.

Aging is an inherent mechanism existing in all living cells. There is a decline in organ functions progressively along with the age-relateddisease development. The twomost important theories related to aging are free radical and mitochondrial theories, and these have passed through the test of time. There is claim by such theories that a vicious cycle is generated within mitochondria wherein reactive oxygen species (ROS) is produced in increased amount thereby augmenting the damage potential [1–6].

The effect of oxidative stress and the interaction of aging and age-related diseases are shown in **Figure 1** [2].

Oxidative stress and disease development are shown in **Figure 2** [3].

Prooxidants are subtances that trigger oxidative stress. Prooxidants are reactive oxygen species (ROS). Prooxidants are studied in two groups. These are exogenous prooxidants and endogenous prooxidants. The exogenous prooxidants are studied in six groups. These are pathogens, drugs, toxicants, dietary ingredients, environmental pollution and climate. The endogenous prooxidants are studied

**Figure 1.** *Effect of oxidative stress and the interaction of aging and age-related diseases.*

#### **Figure 2.**

*Oxidative stress and disease development.*

in seven groups. These are endogenous metabolites, drug metabolites, cellular metabolism, ion flux, anxiety, pathophysiology and ischemia.

Antioxidants are studied in two groups. These are endogenous antioxidants and exogenous antioxidants. Endogenous antioxdants are studied under two groups. These are enzymatic antioxidants and non-enzymatic antioxidants. Dietary antioxidants derived from fruits, vegetables and grains are exogenous antioxidants.

**121**

**2. Prooxidants**

*Reactive oxygen species.*

*GSO* .

*GSO OO*.

**Table 1.**

*2.1.1 Pathogens*

parasite.

*2.1.2 Drugs*

*2.1.3 Toxicants*

oxidative stress.

**2.1 Exogenous prooxidants**

Reactive oxygen species that cause oxidative stress are called prooxidants. Prooxidants occur for a variety of reasons. These may be internal or external causes.

*GSOO*. Glutathione thiylperoxyl radical

<sup>2</sup> Sulfonyl-peroxyl radical

<sup>2</sup> Sulfonyl radical *GSO*. Sulfinyl radical

Pathogens are divided into four groups. These are bacteria, virus, fungus and

Common over-the-counter drug like analgesic (paracetamol) or anticancerous

Toxicants are the man-made harmful substances such as insecticides and many other industrial chemicals which are released to the environment by human activities. Carcinogens, mutagens, allergens, neurotoxin and endocrine disrupters are the different types of toxicants 2 [7]. As an external factor, various toxicants cause

Prooxidants are of two types, exogenous and endogenous.

drug (methotrexate) causes oxidative stress.

*Antioxidant and Oxidative Stress*

*O*.<sup>−</sup>

*O*. 2<sup>−</sup>

*O* 1

*DOI: http://dx.doi.org/10.5772/intechopen.96643*

<sup>2</sup> Superoxide radical

*H O*2 2 Hydrogen peroxide *HO*. Hydroxyl radical *OH*<sup>−</sup> Hydroxyl ion

<sup>2</sup> Singlet oxygen *HOO*. Hydroperoxyl radical *LOOH* Alkylhydroperoxide *LOO*. Alkylperoxyl radical *LO*. Alkoxyl radical *ClO*<sup>−</sup> Hypochlorite ion *Fe O*<sup>4</sup><sup>+</sup> Ferryl ion *Fe O*<sup>5</sup><sup>+</sup> Periferryl ion *NO*. Nitric oxide *HOCl* Hypochlorous acid

<sup>2</sup> Peroxide

Contact of living cells with oxygen leads to the formation of reactive oxygen species. Reactive oxygen species are listed in **Table 1** [1–5].


**Table 1.**

*Reactive oxygen species.*

## **2. Prooxidants**

Reactive oxygen species that cause oxidative stress are called prooxidants. Prooxidants occur for a variety of reasons. These may be internal or external causes. Prooxidants are of two types, exogenous and endogenous.

## **2.1 Exogenous prooxidants**

## *2.1.1 Pathogens*

Pathogens are divided into four groups. These are bacteria, virus, fungus and parasite.

## *2.1.2 Drugs*

Common over-the-counter drug like analgesic (paracetamol) or anticancerous drug (methotrexate) causes oxidative stress.

## *2.1.3 Toxicants*

Toxicants are the man-made harmful substances such as insecticides and many other industrial chemicals which are released to the environment by human activities. Carcinogens, mutagens, allergens, neurotoxin and endocrine disrupters are the different types of toxicants 2 [7]. As an external factor, various toxicants cause oxidative stress.

## *2.1.4 Dietary ingredients*

Dietary ingredients are divided into four groups. These are lipids, carbohydrates, highly processed food and antioxidants.

## *2.1.5 Environmental pollution*

Environmental pollution are divided into three groups. These are transition metals, pesticides and drug residues.

## *2.1.5.1 Transition metals*

These are magnesium, iron, copper, zinc, and so forth.

## *2.1.5.2 Pesticides*

These are BHC, DDT, and so forth.

## *2.1.5.3 Drug residues*

Drug residues cause environmental pollution and trigger oxidative stress.

## *2.1.6 Climate*

Climatic effects such as extreme heat, extreme cold etc. cause oxidative stress.

## *2.1.7 Cigarette smoke*

Cigarette smoke accumulates neutrophils and macrophages in the lungs. Therefore, it is a factor that activates the oxidant mechanism in the body [8].

## *2.1.8 Ozone exposure*

The exposure of the body's airways to ozone causes lipid peroxidation and neutrophil flow in the airway epithelium [9].

## *2.1.9 Hyperoxia*

Hyperoxia is the condition in which the lungs and other tissues have higher oxygen levels. It causes reactive oxygen species (ROS) and reactive nitrogen species (RNS) to form in the body [10, 11].

## *2.1.10 Ionizing radiation*

Ionizing radiation transforms hydroxyl radical, superoxide and organic radicals into hydrogen peroxide and organic hydroperoxides by the effect of *O*2 . These hydroperoxide species react with redox active metal ions such as Fe and Cu. Thus, they cause oxidative stress [12, 13].

## *2.1.11 Heavy metal ions*

Heavy metal ions such as cadmium, mercury, nickel, lead and arsenic cause reactive oxygen species in the body.

**123**

*2.2.4 Ion flux*

*Antioxidant and Oxidative Stress*

**2.2 Endogenous prooxidants**

*2.2.1 Endogenous metabolites*

*2.2.2 Drug metabolites*

*2.2.3 Cellular metabolism*

*DOI: http://dx.doi.org/10.5772/intechopen.96643*

tion, condensation, isomerization [17].

growth, proliferation and preservation of structures.

which these precursors combine to form complex molecules.

the cell's response to environmental changes.

Endogenous metabolites are defined as substrates or products of approximately one thousand nine hundred metabolic enzymes encoded in our genome [14–16]. There are several studies showing that most of these metabolites are toxic. These toxic metabolites are classified depending on the method of introducing toxicity to cells. They are expressed as ROS-producing metabolites, reactive metabolites, metabolite analogues, excitotoxins, and not established/unknown biology.

Drug metabolism is the term used to describe the biotransformation of pharmaceutical substances in the body so that they can be eliminated more easily. The majority of metabolic processes that involve drugs occur in the liver, as the enzymes that facilitate the reactions are concentrated there. The purpose of metabolism in the body is usually to change the chemical structure of the substance, to increase the ease with which it can be excreted from the body. Drugs are metabolized through various reactions including: Oxidation, reduction, hydrolysis, hydration, conjuga-

Cellular metabolism is chemical reactions that occur in living things. They are controlled biochemical reactions in metabolism. Biochemical reactions provide

Cellular metabolism is examined as two processes as anabolism and catabolism. Anabolism is referred to as the constitutive metabolic process. In other words, it is a metabolic process in which a cell uses energy to build various molecules such as enzymes and nucleic acids and to maintain the necessary vital activities. Anabolism consists of three basic stages: The first is the process of making precursors such as amino acids, monosaccharides, isoprenoids, and nucleotides. Second, it includes the process by which precursors such as amino acids, monosaccharides, isoprenoids, and nucleotides are activated to reactive forms. Third, it involves the process by

Catabolism constitutes the second part of the metabolic process. It is the process by which complex molecules are broken down by the cell. Reactions in catabolism provide the energy and substances needed by reactions in anabolism. Catabolic reactions are generally exothermic reactions. Catabolism is divided into several subgroups.

Ion channels are pore-forming proteins that warrant controlled and directed

These are carbohydrate catabolism, fat catabolism and protein catabolism [18].

flux of ions through membranes. Temporal and spatial coordination of ion movements is essential for a wide range of physiological processes including the generation and propagation of the membrane action potential that is critical for

Thanks to the chemical reactions that occur in metabolism, one chemical is transformed into another chemical under the influence of various enzymes. Enzymes direct chemical processes in living things and are indispensable for living things. Enzymes design down to the finest detail the process of homeostasis called

## **2.2 Endogenous prooxidants**

## *2.2.1 Endogenous metabolites*

Endogenous metabolites are defined as substrates or products of approximately one thousand nine hundred metabolic enzymes encoded in our genome [14–16]. There are several studies showing that most of these metabolites are toxic. These toxic metabolites are classified depending on the method of introducing toxicity to cells. They are expressed as ROS-producing metabolites, reactive metabolites, metabolite analogues, excitotoxins, and not established/unknown biology.

## *2.2.2 Drug metabolites*

Drug metabolism is the term used to describe the biotransformation of pharmaceutical substances in the body so that they can be eliminated more easily. The majority of metabolic processes that involve drugs occur in the liver, as the enzymes that facilitate the reactions are concentrated there. The purpose of metabolism in the body is usually to change the chemical structure of the substance, to increase the ease with which it can be excreted from the body. Drugs are metabolized through various reactions including: Oxidation, reduction, hydrolysis, hydration, conjugation, condensation, isomerization [17].

## *2.2.3 Cellular metabolism*

Cellular metabolism is chemical reactions that occur in living things. They are controlled biochemical reactions in metabolism. Biochemical reactions provide growth, proliferation and preservation of structures.

Thanks to the chemical reactions that occur in metabolism, one chemical is transformed into another chemical under the influence of various enzymes. Enzymes direct chemical processes in living things and are indispensable for living things. Enzymes design down to the finest detail the process of homeostasis called the cell's response to environmental changes.

Cellular metabolism is examined as two processes as anabolism and catabolism. Anabolism is referred to as the constitutive metabolic process. In other words, it is a metabolic process in which a cell uses energy to build various molecules such as enzymes and nucleic acids and to maintain the necessary vital activities. Anabolism consists of three basic stages: The first is the process of making precursors such as amino acids, monosaccharides, isoprenoids, and nucleotides. Second, it includes the process by which precursors such as amino acids, monosaccharides, isoprenoids, and nucleotides are activated to reactive forms. Third, it involves the process by which these precursors combine to form complex molecules.

Catabolism constitutes the second part of the metabolic process. It is the process by which complex molecules are broken down by the cell. Reactions in catabolism provide the energy and substances needed by reactions in anabolism. Catabolic reactions are generally exothermic reactions. Catabolism is divided into several subgroups. These are carbohydrate catabolism, fat catabolism and protein catabolism [18].

## *2.2.4 Ion flux*

Ion channels are pore-forming proteins that warrant controlled and directed flux of ions through membranes. Temporal and spatial coordination of ion movements is essential for a wide range of physiological processes including the generation and propagation of the membrane action potential that is critical for the biomechanical activity of muscle cells. Despite their well-established canonical electrophysiological functions in the heart, recent findings have demonstrated that ion channels also might feature ion flux independent functions during heart development and morphogenesis long before acting as ion-conducting pores [19].

#### *2.2.5 Anxiety*

Tension and apprehension cause anxiety. Anxiety disorders can cause low antioxidant defenses and increased oxidative damage to proteins, lipids and nucleic acids pores [20].

#### *2.2.6 Pathophysiology*

Pathophysiology means the examination of the causes of the disease, the various effects caused by the disease, and the abnormal changes in body functions that occur with the disease process. Research in the field of pathophysiology has often focused on physical, mental or psychophysiological states that are directly related to disease processes. Topics such as changes in the endocrine system, changes in certain neurotransmitters, or changes in inflammatory parameters related to the activity of the immune system are examples of research in the field of pathophysiology [21].

#### *2.2.7 Ischemia*

Ischemia is any reduction in blood flow resulting in decreased oxygen and nutrient supplies to a tissue. Ischemia may be reversible, in which case the affected tissue will recover if blood flow is restored, or it may be irreversible, resulting in tissue death [22].

#### *2.2.8 Physical exercise*

Several studies have demonstrated that intense physical exercise causes oxidative stress in animals and humans, being possibly related, for instance, to fatigue and tissue lesions [23].

#### *2.2.9 Antioxidants*

There are a number of reasons why high concentrations of antioxidants may be harmful. At high concentrations, antioxidants may act as pro-oxidants, increasing oxidation; protect dangerous cells (such as cancer cells) as well as healthy cells; reduce the health benefits of exercise; have unwanted side effects, such as nausea and headaches, or even reach toxic levels [1–5, 24].

### **3. Antioxidants**

Antioxidants are examined under two headings as natural and synthetic. Natural antioxidants are examined in two groups as enzymatic and non-enzymatic antioxidants.

#### **3.1 Synthetic antioxidants**

Synthetic antioxidants make up only one analogue (type) of natural antioxidants and are developed to mimic the most effective analogue of the natural antioxidant. Butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), ethoxyquin, propyl gallat and tertiary butylhydroxyquinone (TBHQ ) are some of the synthetic antioxidants.

**125**

*Antioxidant and Oxidative Stress*

**3.2 Natural antioxidants**

*3.2.1 Exogenous antioxidants*

*3.2.1.1 Vitamins*

*3.2.1.2 Trace elements*

*3.2.1.3 Carotenoids*

*3.2.1.4 Phenolic acids*

*3.2.1.5 Flavonols*

*3.2.1.6 Flavanols*

*3.2.1.7 Anthocyanidins*

tute anthocyanidins.

*3.2.1.8 Isoflavones*

*3.2.1.9 Flavanones*

*3.2.1.10 Flavones*

**Figure 3** [4].

zeaxanthin.

*DOI: http://dx.doi.org/10.5772/intechopen.96643*

Exogenous antioxidants are prinicipal dietary antioxidants from fruits, vegetables and grains. Exogenous antioxidants are studied in ten groups.

Vitamins are examined in two groups as vitamin C and vitamin E.

Trace elements are examined in two groups as zinc and selenium.

Carotenoids are examined in four groups as β-carotene, lycopene, lutein and

Chlorogenic acids, gallic acid, cafeic acid, etc. constitute phenolic acids.

Quercetin (and their glucosides), kaempferol (and their glucosides) and

Cyanidin (and their glucosides) and pelagonidin (and their glucosides) consti-

Genistein (and their glucosides), daidzein (and their glucosides) and glycitein

Naringenin (and their glucosides), eriodictyol (and their glucosides) and

luteolin (and their glucosides) and apigenin (and their glucosides) constituteflavones. The beneficial and harmful effects of exogenous antioxidants are shown in

myricetin (and their glucosides) constitute flavonols.

(and their glucosides) constitute isoflavones.

hesperetin (and their glucosides) constitute flavanones.

Proanthocyanidins and catechins constitute flavanol.

## **3.2 Natural antioxidants**

## *3.2.1 Exogenous antioxidants*

Exogenous antioxidants are prinicipal dietary antioxidants from fruits, vegetables and grains. Exogenous antioxidants are studied in ten groups.

## *3.2.1.1 Vitamins*

Vitamins are examined in two groups as vitamin C and vitamin E.

## *3.2.1.2 Trace elements*

Trace elements are examined in two groups as zinc and selenium.

## *3.2.1.3 Carotenoids*

Carotenoids are examined in four groups as β-carotene, lycopene, lutein and zeaxanthin.

## *3.2.1.4 Phenolic acids*

Chlorogenic acids, gallic acid, cafeic acid, etc. constitute phenolic acids.

## *3.2.1.5 Flavonols*

Quercetin (and their glucosides), kaempferol (and their glucosides) and myricetin (and their glucosides) constitute flavonols.

## *3.2.1.6 Flavanols*

Proanthocyanidins and catechins constitute flavanol.

## *3.2.1.7 Anthocyanidins*

Cyanidin (and their glucosides) and pelagonidin (and their glucosides) constitute anthocyanidins.

## *3.2.1.8 Isoflavones*

Genistein (and their glucosides), daidzein (and their glucosides) and glycitein (and their glucosides) constitute isoflavones.

## *3.2.1.9 Flavanones*

Naringenin (and their glucosides), eriodictyol (and their glucosides) and hesperetin (and their glucosides) constitute flavanones.

## *3.2.1.10 Flavones*

luteolin (and their glucosides) and apigenin (and their glucosides) constituteflavones. The beneficial and harmful effects of exogenous antioxidants are shown in **Figure 3** [4].

**Figure 3.** *Beneficial and harmful effects of exogenous antioxidants.*

## *3.2.2 Endogenous antioxidants*

Endogenous antioxidants are studied in two groups: enzymatic antioxidants and non-enzymatic antioxidants.

## *3.2.2.1 Enzymatic antioxidants*

Enzymatic antioxidants are studied in five groups.

*3.2.2.1.1 Superoxide dismutase (SOD)*

Superoxide dismutase (SOD) is enzyme detoxifying superoxide radical (*O*.<sup>−</sup> <sup>2</sup> ).

*3.2.2.1.2 Catalase (CAT) and glutathione peroxidase (GPx)*

Catalase (CAT) and glutathione peroxidase (GPx) are enzymes involved in the detoxification of peroxides (CAT against *H O*2 2 and GPx against both *H O*2 2 and *ROOH* ).

## *3.2.2.1.3 Glutathione reductase*

Glutathione reductase is enzyme involved in the regeneration of glutathione.

## *3.2.2.1.4 Thioredoxin reductase*

Thioredoxin reductase is enzyme involved in the protection against protein oxidation.

**127**

**Author details**

Betül Çalişkan1

Denizli, Turkey

\* and Ali Cengiz Çalişkan<sup>2</sup>

from causing DNA, lipid and protein damage.

\*Address all correspondence to: bcaliska@gmail.com

provided the original work is properly cited.

1 Department of Physics, Faculty of Arts and Science, Pamukkale University,

2 Department of Chemistry, Faculty of Science, Gazi University, Ankara, Turkey

© 2021 The Author(s). Licensee IntechOpen. This chapter is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/ by/3.0), which permits unrestricted use, distribution, and reproduction in any medium,

*Antioxidant and Oxidative Stress*

of NADPH.

**4. Conclusion**

*DOI: http://dx.doi.org/10.5772/intechopen.96643*

*3.2.2.1.5 Glucose-6-phosphate dehydrogenase*

Glucose-6-phosphate dehydrogenase is enzyme involved in the regeneration

Glutathione (GSH), uric acid, lipoic acid, NADPH, coenzyme Q, albumin and

Oxidative stress is a condition that occurs with the increase of free radicals. Free radicals tend to increase in the body for various reasons. This situation may be caused by exogenous reasons or by various changes in the body (endogenous). As a result of the oxidation process in the body, reactive oxygen species (ROS) are formed. In addition to reactive oxygen species, reactive nitrogen species are also formed in the body. The oxidation process begins with the introduction of food into the body. Oxidation is a process that can be both beneficial and harmful. Oxidation triggers the formation of free radicals, ie reactive oxygen species. If antioxidants do not come into play as a balance element in the body, the increase in free radicals damages the body and causes the formation of the disease process. Depletion of various substances in the body due to age also triggers the formation of reactive oxygen species and the emergence of various diseases. Antioxidants can be various external substances, as well as various enzymes in the body and non-enzymatic substances in the body. With the effect of antioxidants, free radicals are prevented

*3.2.2.2 Non-enzymatic antioxidants (principal intracellular reducing agents)*

bilirubin make up the non-enzymatic antioxidant class [1–5].

## *3.2.2.1.5 Glucose-6-phosphate dehydrogenase*

Glucose-6-phosphate dehydrogenase is enzyme involved in the regeneration of NADPH.

*3.2.2.2 Non-enzymatic antioxidants (principal intracellular reducing agents)*

Glutathione (GSH), uric acid, lipoic acid, NADPH, coenzyme Q, albumin and bilirubin make up the non-enzymatic antioxidant class [1–5].

## **4. Conclusion**

Oxidative stress is a condition that occurs with the increase of free radicals. Free radicals tend to increase in the body for various reasons. This situation may be caused by exogenous reasons or by various changes in the body (endogenous). As a result of the oxidation process in the body, reactive oxygen species (ROS) are formed. In addition to reactive oxygen species, reactive nitrogen species are also formed in the body. The oxidation process begins with the introduction of food into the body. Oxidation is a process that can be both beneficial and harmful. Oxidation triggers the formation of free radicals, ie reactive oxygen species. If antioxidants do not come into play as a balance element in the body, the increase in free radicals damages the body and causes the formation of the disease process. Depletion of various substances in the body due to age also triggers the formation of reactive oxygen species and the emergence of various diseases. Antioxidants can be various external substances, as well as various enzymes in the body and non-enzymatic substances in the body. With the effect of antioxidants, free radicals are prevented from causing DNA, lipid and protein damage.

## **Author details**

Betül Çalişkan1 \* and Ali Cengiz Çalişkan<sup>2</sup>

1 Department of Physics, Faculty of Arts and Science, Pamukkale University, Denizli, Turkey

2 Department of Chemistry, Faculty of Science, Gazi University, Ankara, Turkey

\*Address all correspondence to: bcaliska@gmail.com

© 2021 The Author(s). Licensee IntechOpen. This chapter is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/ by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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**131**

**Chapter 7**

**Abstract**

Rescue?

with newest literatures.

vitamins

**1. Introduction**

drawing great attention for scientific studies.

*Cenk Aydin and Nilay Seyidoglu*

Natural Antioxidants to the

Natural antioxidant compounds have different mechanisms of treatment and prevention against various diseases due to their richest ingredients. There are several antioxidants used today, such as phytogenic ingredients, flavonoids, capsaicin, spirulina, beta-glucan, polyphenol etc. Besides the outbreak of diseases, the ability to scavenge oxidative conditions of the natural antioxidants have been notably important. Thereby, therapeutic strategies of diseases have been interested by researchers. Try to seek a kind of effects of natural antioxidants to various diseases, especially viral or pandemic diseases are being important nowadays. This chapter we'll mention about how to viral or pandemic disease's effects on oxidative status in both animals and humans, and what kind of phytochemical ingredients would be a positive effect on. At the same time, the latest advances about these natural antioxidant compounds and pharmaceuticals will be critically highlighted and discussed

**Keywords:** natural antioxidants, phytogenic ingredients, essential oils, pandemic,

Antioxidants either endogenous or exogenous are essential substances to regulate the oxidative process. The endogenous antioxidants known as glutathione, glutathione peroxidase (GPx), catalase (CAT), and superoxide dismutase (SOD), have an inhibition role on free radicals during oxidation [1]. Nevertheless, either these antioxidant mechanisms are inadequate or for a better healthy life, exogenous antioxidants should be preferred [2]. The exogenous antioxidants which are especially called natural antioxidants such as vitamins, flavonoids, polyphenols, minerals, plants and phytochemicals, are derived from foods and medicinal plants. Over the years, the relationship between natural antioxidants and health has been discussed due to their important efficiencies. Generally, natural antioxidants have a wide range of effects especially antioxidant, antimicrobial, anticancer, immunomodulatory, and antiviral [3–5]. Belong these, natural antioxidants have been

There are several multidisciplinary studies with natural antioxidants that have focused on human and animal experiments. The researchers have examined to understand the mechanisms of antioxidants on oxidative stress. They have been widely documented the knowledge of free radicals with antioxidants by identifying the oxidative stress pathway, cellular efficiencies and general health impact [6, 7].

## **Chapter 7**
