**4. Conclusion**

In conclusion, a growth the good-quality macromolecular crystal is crucial for the determination of its atomic structure using of X-ray crystallography. Several effective advanced methods leading to the formation of crystal in diffraction quality are available. Nowadays, the extensive improvement of X-ray facilities enables data collection from smaller crystals and allows using the new trends in crystallization strategies that are developing as a powerful advanced crystallization approach.

The protein crystallization manufacturers such as Hampton Research, Molecular Dimensions Ltd., PerkinElmer, GE Healthcare, Danaher, Bruker, Agilent, Jena Bioscience, Rigaku, Formulatrix and MiTeGen are basic companies responsible for systematic invention of new products and technologies that constantly improve protein crystallization methods and are looking for crystallization techniques for hardly crystallisable proteins such as membrane proteins.

Next essentials that have an effort to improve crystallization techniques are attempts to use crystals in different non-conventional ways. One of them is sending biosubstances (protein-based medicines) into space to try to crystallize them for finding new uses of crystallized proteins as pharmacotherapeutics. These protein crystals can be supposedly thereafter used for long-term storage or for stabilization of biosubstances by transferring them into solid state (Bristol-Myers

*Advanced Biocrystallogenesis DOI: http://dx.doi.org/10.5772/intechopen.97162*

Squibb Company). Another use of crystallization was reported in material sciences, more precisely for improvement of electronic materials developed on the base of biomolecules arrangement in crystals [65].

These and many more applications of protein crystallization are reasons for development of new methods and strategies for faster crystallization and obtaining of well diffractive crystals and for reduction of protein volume. However, there is still a necessity for improvement and new methods and strategies for biomolecules crystallization awaits to be discovered. Although this can be achieved more easily because biochemistry, biotechnology, physics, chemistry and applied nanosciences are extra overlapped and allow to improve and explain in depth the process of protein crystallization.
