**7. Quantitative analysis of phytochemicals**

From the nutritional composition of *A. marmelos*, it is well known that the fruit is highly rich in various bioactive phytochemicals. It has been observed from various research studies that different types of phytochemicals or bioactive compounds are present in *A. marmelos*. Main class of phytochemicals are generally estimated as total phenolic count (TPC), total flavonoid content (TFC), and total carotenoids (TC). TPCs are responsible for anticancer activity of *A. marmelos*. For estimation, different processes are used, and for a particular process, there is taken a compound as a standard. For example, Folin–ciocalteu (FC) assay is used for TPC estimation with gallic acid as a standard. Similarly, TC is estimated by Gross method, and TFC estimation involves AlCl3 colorimetric assay where rutin or catechin standard is used. Pearson's method is generally used for the determination of ascorbic acid, and for total alkaloids ammonium hydroxide precipitation method is used. In pulp, TPC 87.3 4 mg GAE/g dw, TFC 15.20 mg CE/g dw (catechin equivalent per gram),

vitamin C 26.1 7 mg /100dw and TC 32.98 μg/g dw is present [53]. In leaf, it has been observed that there are present TFC 63.9 mg/g, total phenolic content 29.4 mg/g, alkaloids 16.0 8 mg/g, and tannins 10.5 4 mg/g [54]. In flower, TFC ethanol extract is found to be 1615.2 mg GAE/g (gallic acid equivalent/g), and aqueous extract is found to be 1496.7 mg GAE/g [55]. In bark, it has been found that total phenolic content is 14.2 mg GAE/g [56].
