**6.5 FTIR spectroscopy**

The FTIR spectroscopy was done by preparing the KBr discs. 1 mg of isolated biomaterial was taken and mixed with 100 mg of dried and desiccated solid powder of potassium bromide. The mixture was uniformly mixed in mortar and pestle and placed in an IR lamp to remove any moisture. The mixture was converted into a disc under the pressure of 10 tons. The prepared disc was placed in the disc holder in the path of infrared radiation. In the range of 4000–200 cm−1, the spectrum was recorded [4, 62] **Figure 2**. Defines the FTIR spectroscopy

**Figure 2.** *IR spectrum of the isolated biomaterial Litchi chinesis.*

of the isolated biopolymer from Litchi chinesis. The biopolymer showed the presence of The IR spectra revealed the presence of secondary alcohol (1099.83 cm−1), aromatic nitro and phenol groups (1316.59 cm−1), aromatic rings (1544.70 cm−1), and the presence of alkenes (1652.02 cm−1), with –CH2 and –COOH stretching (2928.35cm−1) along with β-diketones and O–NO2 (1652.02 cm−1). The presence of these functional groups were found to be similar with the functional groups observed in standard polymers. This functional groups revealed its polymeric nature [4].
