**3.8 Isolation and characterization of biopolymers**

	- a.Physical appearance, taste, shape, and texture.
	- b.Solubility study.
	- c.Particle size analysis by optical microscopy.
	- d.Bulk density determination.
	- e.Tapped density.
	- f. The angle of repose.
	- g.Percentage consolidation Index.
	- h.Melting point testing.
	- i. Chemical test.
	- j. SEM analysis.
	- k.FTIR spectroscopy.

### **3.9 Isolation of biomaterial from** *the natural source*

200 gm of *natural sources like* seeds, bark, fruits, legumes, kernels, flowers are soaked overnight in purified water. The upper covering of the almond was then removed. About 100 ml of water was added to this and this mixture was mixed in a mixer. This slurry was filtered with the help of muslin cloth and thus the biomaterial was separated by filtration. The resultant was obtained as filtrate. After that, the mixture was subjected to centrifugation at about 4000 rpm for 15 minutes and then the resultant supernatant layer was properly separated and taken. Then acetone was added in the ratio of 1:1 and mixed properly. This mixture was kept in the refrigerator overnight at 4°C and the solution was centrifuged at 4000 rpm for 30 minutes. The residue was collected having biomaterial and dried in desiccators for 24 hours. This residue of biomaterial was washed with acetone and the biomaterial was dried naturally for 10 hrs for getting freeflowing powder. The collected biomaterial for stored in airtight containers after passing through sieve no. 120 for further use. The schematic flow chart of the isolation procedure has been summarized in Flow chart 4.1. This procedure was repeated six times and optimized and then the percentage yield was calculated and reported [6, 25].
