**3.2 Novel sonication method for nanoparticles preparation**

The bionanosuspension can be prepared by a novel method called the sonication method [13]. In this method, the biopolymer as bio stabilizer cum bioretardant was mixed with other ingredients like a preservative, surface active agent like PVA, nanosizent with the distilled water to make a well-dispersed suspension [14]. Then the mixture was subjected to bath sonication for 10–15 cycles to formulate the nanosized drug-loaded bionanosuspension [15].
