**2.12 Tumor extract preparation**

Tumors treated with NC-siRNA or with combined siRNA-HDAC8 + siRNA-MYC were cut into small pieces and homogenized in assay buffer in a glass homogenizer. Assay buffer as described by Khandelia et al. [16], consisted of 20 mM Tris–HCL pH 7.5, 150 mM NaCl, 5 mM EDTA, 10% glycerol, 1% Nonidet P40, and protease inhibitor cocktail from Sigma (P8340). Protein concentrations were determined using Pierce's BCA Assay as per the manufacturer's instructions.

## **2.13 Statistical analysis**

Error bars represent standard error of the mean (SEM) from 2 to 3 biological replicates from 3 to 5 independent experiments. P-values were calculated using T.Test (2 tailed, 3 samples, unequal variance) and p < 0.05 was considered statistically significant.
