3.Sequence alignment using BLAST

4.Physico-chemical parameters including secondary structure, charge, size, hydrophobicity and amphiphilic character as these yielded an appreciable accuracy using machine learning technique. The values of physico-chemical properties were retrieved from AA index database (Kawashima and Kanehisa 2000)

The three-dimensional model of the peptides was fabricated by Chimera 1.8.1 software.

ToxinPred tool was run with default parameters to calculate toxicity prediction of the determined peptides. All 25 peptides were non-toxic [30].

#### **Figure 1.**

*Structures of EBVO proteins obtained from PDB; (A) GP; (B) L, (C) NP, (D) VP24, (E) VP30, (F) VP35, (G) VP40.*

*Docking-Based Screening of Cell-Penetrating Peptides with Antiviral Features and Ebola Virus… DOI: http://dx.doi.org/10.5772/intechopen.97222*
