**6. Factors affecting bactericidal potential of AgNP's**

Primarily morphology i.e. size and shape along with reactivity of AgNP's were responsible for bactericidal potential of AgNP's. Size and surface are inversely proportional to each other as size decreases area increases leads of rapid rise in surface-area to volume ratio. Bactericidal potential inhibit cell wall and free radicals Ag-thiol groups of enzymes Preventing biofilm formation Intercalates between bases Attaching to the surface of the cell membrane Bacterial peptides that can affect cell signaling Attaches to 30 s subunit (**Figure 3**). Silver nanoparticles showing multiple bactericidal actions [74].
