**2.2 Synthesis of silver nitrate (AgNO3) solution**

The silver nitrate solution is prepared by using 1.698 g silver nitrate powder in 100 ml of distilled water to form 100 mM concentration. From his prepared 100 ml of silver nitrate solution; 1 ml is used for preparation of 1 mM silver nitrate solution for silver nanoparticles synthesis. The complete process is performed under absence of light.

#### **Figure 3.**

*Displays the synthesis of dichlorofluorescein silver nanoparticles (DCF-SNP) employing AgNO3 and dichlorofluorescein under heating conditions. The change in color of the reaction mixture was noticed from light green to dark brown, demonstrating the formation of silver nanoparticles.*

#### **2.3 Synthesis of dichlorofluorescein silver nanoparticles**

The dichlorofluorescein silver nanoparticles (DCF-SNPs) is prepared by mixing 97.5 ml of AgNO3 (1 mm) and 2.5 ml of dichlorofluorescein in conical flask at constant stirring. The complete reaction process is carried out under boiling condition for 1–2 min, the pH of the mixture is adjusted to 12 and the reaction completes after 24 hours. The chemical constituents or the functional group that are presents in the Dichlorofluoresceine (DCF) structure reduced the silver salt to form the silver nanoparticles called as dichlorofluorescein silver nanoparticles (DCF-SNPs). The complete process of synthesis of dichlorofluorescein silver nanoparticles (DCF-SNPs) using AgNO3 and dichlorofluorescein at 0 hr., 2 hr. and 24 hr. and observed changes in color from light green to dark brown, confirming the synthesis of silver nanoparticles synthesis is given in **Figure 3**.
