*2.1.6 DNA nanomaterials*

Y-DNA was prepared by mixing equimolar amounts of three single stranded DNA (ssDNA), two long and one short. The two long sequences have regions that hybridize to the shorter one. One of the fields is not completely linked to the corresponding fragment. Thus, the target miRNA became able to replace this fragment and remove the Y-DNA nanostructure. ssDNAs were dissolved in hybridization buffer at 10 μM final concentration per sequence and annealed to form the desired Y-shaped DNA: annealed at 95°C for 2 minutes, cooled to 65°C and incubated for 5 minutes, followed by 2 minutes while its temperature dropped to 60°C and cooled to 20°C at a rate of 1° per minute. The final products were stored at 4°C. Double stranded substrates were formed by mixing in the hybridization buffer. The mixture was heated to 95°C for 5 minutes and slowly cooled to 4°C, then allowed to stand at room temperature for 20 minutes to form a specific double stranded substrate [13].
