*2.1.7 DNAzyme*

DNA phosphorylation was made by incubating 200 pmol of FS1 with 20 units of T4 polynucleotide kinase (PNK) at 37°C for 30 min in a 100 μL reaction mixture containing 50 mM Tris–HCl (pH 7.6 at 25°C), 10 mM MgCl2, 5 mM 1,4-Dithiothreitol (DTT), 0.1 mM spermidine and 1 mM adenosine 5′-triphosphate (ATP). The reaction was stopped by heating the mixture at 90° C for 5 minutes. RFT1 (100 μM) and 2 μL RFS1 (100 μM) were then added to the solution, and the mixture was heated to 90°C for 40 seconds and cooled to room temperature for 10 minutes. In the last step, 10 units of T4 DNA ligase were added for DNA ligation at 25°C for 2 hours. The ligation mix contains 10 mM MgCl2, (150 μL) 40 mM Tris– HCl (pH 7.6 at 25°C), 10 mM DTT and 0.5 mM ATP. The products were concentrated by standard ethanol precipitation and further purified by polyacrylamide gel electrophoresis [14].

## *2.1.8 Carbon Nanodots*

The syntheses of carbon nanodots (CDs) will describe according to the literature [15].

CDs were synthesized hydrothermally with citric acid and ethylenediamine (EDA). Initially citric acid (3.0 g) and ethylenediamine (1875 μL) were dissolved in 30 mL of distilled water. The solution was then transferred to a 500 mL round bottom flask and heated at 150°C for 5 hours. The product was dialyzed against ddH2O to obtain CDs. CDs powder was obtained by evaporating, redispersed in deionized water, and stored at 4°C for later use [15].
