**Funding**

This work was supported by Ministry of Health of the Czech Republic (grant no. NV18-08-00299) and by Masaryk University (MUNI/A/1421/2019, MUNI/A/1382/2019). All rights reserved.

**133**

*Intact Cell Mass Spectrometry for Embryonic Stem Cell Biotyping*

MBT 2-mercaptobenzothiazole MEFs mouse embryonic fibroblasts mESCs mouse embryonic stem cells PCA principal component analysis

Petr Vaňhara1,2\*, Lukáš Moráň1,3, Lukáš Pečinka2,4, Volodymyr Porokh1

1 Department of Histology and Embryology, Faculty of Medicine, Masaryk

2 International Clinical Research Center, St. Anne's University Hospital, Brno,

4 Department of Chemistry, Faculty of Science, Masaryk University, Brno,

5 Department of Chemical and Geological Sciences, University of Cagliari,

6 Department of Analytical Chemistry, Nutrition and Food Science, Faculty of

© 2020 The Author(s). Licensee IntechOpen. This chapter is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/ by/3.0), which permits unrestricted use, distribution, and reproduction in any medium,

3 Research Centre for Applied Molecular Oncology (RECAMO), Masaryk Memorial

, Eladia Maria Peña-Méndez6

, Aleš Hampl1,2 and Josef Havel2,4

MALDI TOF MS matrix-assisted laser desorption/ionization time-of-flight

mass spectrometry

PLS-DA partial least squares discriminant analysis

, Sebastiano Masuri<sup>5</sup>

Chemistry, University of La Laguna, Tenerife, Spain

provided the original work is properly cited.

\*Address all correspondence to: pvanhara@med.muni.cz

,

,

*DOI: http://dx.doi.org/10.5772/intechopen.95074*

ICM inner cell mass

SA sinapinic acid

STR short tandem repeats.

**Author details**

Tiziana Pivetta5

Czech Republic

Czech Republic

Monserrato, CA, Italy

José Elías Conde González6

University, Brno, Czech Republic

Cancer Institute, Brno, Czech Republic
