**2. RNA sequencing**

RNA sequencing is the molecular technique in which the quantity as well as the sequence of RNA in a biological sample tissue of choice is determined using next generation sequencing. Here, the coding and noncoding RNAs or in short the transcriptome of the gene is analyzed. It was first described a decade ago. During the infant period of this technique, the Sanger sequencing technology was used in RNA sequencing which is greatly replaced in the present by more accurate next generation sequencing technology. RNA sequencing is found to be superior to many other techniques especially tissue microarray hybridisation [7]. The proposed advantages of the former over the later are:

