*2.1.1 Preparation of CD/Chitosan composite film*

For fabrication of the chitosan/CDs nanocomposite, 3 wt% homogenous solution was prepared by dissolving chitosan in 0.3 M acid acetic. The sterile square chitosan patterns were produced using a standard square mold with the size of 30 × 40 mm. Various proportions of CDs, including The films were designated as CD/Ch(0), CD/Ch (1), CD/Ch (2) and CD/Ch (3) and Ch/CD(4) were gently added to the chitosan solution to prepare the desirable antibacterial mixture. Respectively, where the number in Parent hesis denotes the volume of CDs solution present in 20 ml of the film forming chitosan solution. Subse- quently, the mixture was injected into the square mold for the formation of composite films. Then, the molds were lyophi- lized in a freeze dryer to complete the molding process.
