**3.11 Cell cytotoxicity studies**

The results of cell cytotoxicity for the plain sample Ch/CD (0) and the CDs loaded sample Ch/CD (2) are shown in **Figure 9(a)** and **(b)** respectively.

As per ISO 10993-5, the achievement of numerical grade more than 2 is considered as toxic. The score was obtained as 0 for both of the samples, thus indicating non-cell cytotoxic nature of both of the film samples. It can be seen that there are discrete intra cyto plasmatic granules, no cell lysis and there is no reduction of cell growth.
