**4. Conclusions**

*In vitro* metabolic stability studies are very important during drug discovery and development to predict the *in vivo* clearance of compounds and to know the number and types of metabolites formed. These studies are also helpful to find out the pharmacological and toxicological profiles of new chemical entities. *In vitro* metabolic stability studies are commonly performed by using liver microsomes and hepatocytes. LC-HRMS has turn into an important tool for the detection and characterization of drug metabolites *in vitro* and in complex biological samples. LC-HRMS, along with data acquisition and post-acquisition data mining techniques facilitated the drug metabolite identification.

*Drug Metabolism*
