**5.18 Toxicological effect**

In an acute toxicity study, the methanolic extract of *P. integerrima* bark proved to be safe [38]. Besides, the *P. atalantica* fruits also proved safe in acute toxicological studies where the acute toxicity was evaluated for two days. Antinociceptive action was conducted with tail-flick, hot plate, and rotarod test. The *P. atlantica* fruit extract levels for LD50 were 1.66 g/kg with a cumulative non-lethal dosage of 0.93 g/kg. The fruit extract derived from *P. atlantica* at the doses range of 50–350 mg/kg conferred analgesic effects dose-dependently 30 minutes after administration during the hot plate and tail-flick tests so that a substantial difference between the groups obtaining saline and the extract was observed (p < 0.05). Results also revealed no significant differences in a sensory-motor assessment with *P. atlantica* fruit extract's administration at doses ranging from 50 to 350 mg/kg. Additionally, findings revealed robust antinociceptive behavior of the *P. atlantica* fruit extract in mice [45].
