**2.2 Technique of injection**

The adipose pads are wrapped by a relatively impermeable fascia that would prevent subcutaneous infections from spreading to the more fragile adipose tissue. It is therefore easy to inject above and under the fat pocket without "wetting" the fat.

As shown in (**Figure 2**), the first and most important injection put the anaesthetic solution in contact with the emerging nerves under the fat deposit just above the muscle aponeurosis using a 3-mm diameter cannula. The second very superficial of lower volume will use a thin 2 mm diameter cannula, immediately under the skin for a good vasoconstriction, under view control, above the fat pocket.

After few minutes the harvesting begins from the core of the fat deposit, generally free of liquids (**Figure 3A** and **B**).

#### **Figure 1.**

*A sketch to figure the Klein' solution composition. [https://sketchymedicine.com/2016/01/ tumescent-solution-for-burn-surgery-and-liposuction/].*

*Liposuction for Fat Transfer: The "Island Technique" DOI: http://dx.doi.org/10.5772/intechopen.96869*

#### **Figure 2***.*

*The first injection is done under the fat along the muscle aponeurosis. The second injection is done immediately under the skin.*

#### **Figure 3.**

*A and B: During the FAMI technique a virtually lidocaine free samples are harvested using 10 cc Luer lock syringes ready to be put in the centrifuge for purification.*
