**4. Fluorescent images and guided surgery**

*Ovarian Cancer - Updates in Tumour Biology and Therapeutics*

(PDT) [21, 22].

**3. Diagnosis and imaging**

**3.1 Targeted imaging agents**

**3.2 Activatable imaging agents**

**3.3 Nano-liposomal imaging agents**

ultrasound [25].

imaging [26].

large surface area, large aspect ratio, nanoscale size stability and multiple chemical functions and they are especially important as carriers for transporting drugs and biomolecules. In this regard, this type has been used due to the functional properties it possesses as an important transporter for the delivery of anti-cancer drugs and many proteins and genes. Likewise, to directly kill cancer cells, it was used as a carrier for photothermal therapy (PTT) and photodynamic therapy

In recent times, there have been many improvements and major developments in the field of diagnosis and imaging with the help of nanotechnology, where it has been used the technologies of biosensors and point of care systems as well as the updated and improved imaging technologies as well as the integration of bioinformatics together with multiplexed assays. At present, there are many nanoparticle platforms and microelectromechanical systems to strengthen and improve diagnostic processes, largely as a means of diagnosing biomarkers and of course by enhancing the contrast agents used in imaging [23]. And the real mechanics of the imaging agent used to improve visualization and accumulation within the target cells in many imaging mechanisms on its subtype. There are different imaging methods that use imaging agents such as Optical Imaging, X-ray Imaging, positron emission

Targeted contrast agents are placed in a specific type of tissue or cellular receptors, including certain types, such as target agents designated for imaging fibrin, which are molecules associated with fibrin, which are molecules associated with fibrin, and other molecules to track stem cells from super magnetic iron oxide, and there are others for imaging angiogenesis, which are of the multimodal type of carbon fluorinated, liposomes are used to target the sclerotic components, and to visualize transplant rejection, microscopic bubbles were used in MRI and

There are many nanoparticles that are actually designed to have better performance and are imaging agents called operable molecular probes that can produce a signal or some kind of change that can be recorded or detected, for example, when enzymatic activity or a specific response to important chemical reactions, Two imaging technologies are combined into a single activatable lifetime imaging agent. This is applied by combining the high specificity of luminescence lifetime imaging with the high signal-to-background ratio of activatable fluorescence

Liposomes can encapsulate biomolecules that are hydrophilic and increase their internalization and solubility through the lipid bilayers of the cells, Among the drawbacks that can occur in the case of imaging by means of high elimination agents and low systemic retention degrees, and because the rapid removal process from the bloodstream or the body reduces the period and efficiency of imaging,

tomography (PET), Magnetic resonance imaging (MRI) [24].

**166**

To increase the sensitivity, efficiency and strength of the imaging techniques used with surgery and increase their clinical efficiency, there is an actual need to develop new material, Therefore, many fluorescent nanoparticles essential for Image-supported surgeries were developed, tested and designed and tested in preclinical surgery there are some examples of that:

