**Appendix A**



#### **Appendix Table 1.**

*The list of genes with significantly upregulated expression of CT antigenes in the cohort of 51 primary melanomas compared to 27 B-RAF V600E-mutant nevi from the NCBI's Gene Expression Omnibus GSE98394 dataset (described in detail in [80]). EdgeR [127] was used to perform differential expression analysis through the generalized linear model approach. The differentially upregulated in melanoma genes (log2FC > 0, p < 0.01) were filtered for CT antigenes. The whole CT antigenes list comprising of 220 genes was acquired from the CT database [70]. Expression is presented as log2 FC units.*

**107**

*The Role of the Meiotic Component in Reproduction of B-RAF-Mutated Melanoma…*

**Gene Symbol Log2FC** SPANX family member A2 SPANXA2 4.748924 SPANX family member B1 SPANXB1 4.617090

Transgelin 3 TAGLN3 4.426952 SPANX family member D SPANXD 3.947176

SPANX family member C SPANXC 3.684016 Interleukin 13 receptor subunit alpha 2 IL13RA2 2.558249 Coiled-coil domain containing 33 CCDC33 2.399770 PAGE family member 4 PAGE4 2.248496 Nucleolar protein 4 NOL4 2.078128 Tudor domain containing 15 TDRD15 1.896378 VENT homeobox pseudogene 1 VENTXP1 1.859120 DDB1 and CUL4 associated factor 12 like 2 DCAF12L2 1.816185 SPANXA2 overlapping transcript 1 SPANXA2-OT1 1.793161 RNA binding motif protein 46 RBM46 1.765131 F-box protein 39 FBXO39 1.599419 ADAM metallopeptidase domain 28 ADAM28 1.545184 T cell activation RhoGTPase activating protein TAGAP 1.530934 Tektin 5 TEKT5 1.443142 Maelstrom spermatogenic transposon silencer MAEL 1.415596 Actin-like 8 ACTL8 1.358688 MAGE family member A1 MAGEA1 1.315031 ADAM metallopeptidase domain 21 ADAM21 1.210916 PRAME N-terminal-like, pseudogene PRAMENP 1.202568 MAGE family member A10 MAGEA10 1.163532 MAGEA10-MAGEA5 readthrough MAGEA10-MAGEA5 1.163181 NLR family pyrin domain containing 4 NLRP4 1.053507 ADAM metallopeptidase domain 22 ADAM22 0.922948 Acrosin binding protein ACRBP 0.854224 Transmembrane protein 108 TMEM108 0.793037 Ankyrin repeat domain 45 ANKRD45 0.779239 BAGE family member 2 BAGE2 0.733106 Mesenteric estrogen dependent adipogenesis MEDAG 0.72858 Sperm associated antigen 4 SPAG4 0.70602

SPANXA1 4.501381

TMEFF2 3.767568

*DOI: http://dx.doi.org/10.5772/intechopen.93641*

Sperm protein associated with the nucleus, X-linked,

Transmembrane protein with EGF-like and two follistatin-

family member A1

like domains 2

*The Role of the Meiotic Component in Reproduction of B-RAF-Mutated Melanoma… DOI: http://dx.doi.org/10.5772/intechopen.93641*


*Melanoma*

**Gene Symbol Log2FC** Melanoma-associated antigen 4 MAGEA4 5.327805 Prostate-associated gene protein 5 PAGE5 5.246215 Prostate-associated gene protein 2 PAGE2 4.905513 MAGE family member B2 MAGEB2 4.803597 Melanoma-associated antigen 11 MAGEA11 3.985815 PAGE family member 2B PAGE2B 3.714482 MAGE family member C1 MAGEC1 3.522569 Melanoma-associated antigen 10 MAGEA10 3.168079 Cancer/testis antigen family 25, member 1a DSCR8 2.770747 Interleukin 13 receptor subunit alpha 2 IL13RA2 2.338307 Transgelin TAGLN 2.28783 Catenin alpha 2 CTNNA2 2.112868 Mesenteric estrogen-dependent adipogenesis MEDAG 2.087961 PDZ binding kinase PBK 2.022606 Homeobox protein BarH-like 1 BARX1 1.99113 Centrosomal protein 55 CEP55 1.86278 Sperm-associated antigen 4 SPAG4 1.521662 T-cell activation RhoGTPase activating protein TAGAP 1.510325 MAGE family member B17 MAGEB17 1.498956 Homeobox protein ARX ARX 1.147917 Outer dense fiber of sperm tails 3B ODF3B 1.144663 ATPase family AAA domain containing 2 ATAD2 1.116308 MAGE family member D1 MAGED1 0.941916 GATA zinc finger domain containing 2A GATAD2A 0.892566 ADAM metallopeptidase domain 28 ADAM28 0.838868 Phosphotyrosine picked threonine-protein kinase TTK 0.78873 Opa-interacting protein 5 OIP5 0.775664 Acrosin binding protein ACRBP 0.518623 Nucleolar protein 4 like NOL4L 0.487608 GATA zinc finger domain containing 2B GATAD2B 0.484958 Outer dense fiber of sperm tails 2 ODF2 0.40552 MAGE family member F1 MAGEF1 0.334573 Cancer/testis antigen 101 KIAA0100 0.315249 Transgelin 2 TAGLN2 0.241303 DDB1- and CUL4-associated factor 12 DCAF12 0.228037

*The list of genes with significantly upregulated expression of CT antigenes in the cohort of 51 primary melanomas compared to 27 B-RAF V600E-mutant nevi from the NCBI's Gene Expression Omnibus GSE98394 dataset (described in detail in [80]). EdgeR [127] was used to perform differential expression analysis through the generalized linear model approach. The differentially upregulated in melanoma genes (log2FC > 0, p < 0.01) were filtered for CT antigenes. The whole CT antigenes list comprising of 220 genes was acquired from* 

*the CT database [70]. Expression is presented as log2 FC units.*

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**Appendix Table 1.**


#### **Appendix Table 2.**

*Significantly upregulated expression of CT antigenes in the cohort of 368 melanoma metastases compared to 103 primary melanomas from the TCGA-SKCM dataset (https://www.cancer.gov/tcga). The data was extracted from the TCGA database using the TCGA Biolinks Bioconductor package [124]. EdgeR [127] was used to perform differential expression analysis through the generalized linear model approach and the differentially expressed genes (DEGs) which were upregulated in metastatic melanoma (log2FC > 0, p < 0.01) were filtered for CT antigens. The CT antigenes list comprising of 220 genes was acquired from the CT database [70]. Expression is presented as log2FC units.*

**109**

**Author details**

and Jekaterina Erenpreisa1,2

Dace Pjanova1

*The Role of the Meiotic Component in Reproduction of B-RAF-Mutated Melanoma…*

\*, Ninel M. Vainshelbaum1,2, Kristine Salmina1

© 2020 The Author(s). Licensee IntechOpen. This chapter is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/ by/3.0), which permits unrestricted use, distribution, and reproduction in any medium,

1 Latvian Biomedical Research and Study Centre, Riga, Latvia

2 Faculty of Biology, University of Latvia, Riga, Latvia

\*Address all correspondence to: dace@biomed.lu.lv

provided the original work is properly cited.

*DOI: http://dx.doi.org/10.5772/intechopen.93641*

*The Role of the Meiotic Component in Reproduction of B-RAF-Mutated Melanoma… DOI: http://dx.doi.org/10.5772/intechopen.93641*
