*2.4.1 Basic analysis*

The 20 shortlisted protein candidates from VVaDK, VFDF and VFDX PIN analysis were subjected to further analyses for predicting the plausible vaccine and/or drug candidates. All such proteins were explored for their molecular weight calculation, cellular localization, signal peptide prediction followed by antigenicity prediction. ProtParam was used to find the molecular weight and number of amino acids [32] and cellular localization was analyzed by PSORTb v3.0.2 [33]. Location of signal peptides was predicted using the server called SignalP 4.1 [34]. Lipoprotein signal peptides were predicted using the LipoP 1.0 [35]. Finally, Vaxijen was used to predict the possible antigenicity of the proteins [36].
