**5. Determination of solubility of isolated compound**

The two isolated compounds were analyzed for their solubility in different solvents as in DMSO, ethanol, methanol and acetone.

#### **6. Melting point determination**

Melting points of two isolated biomolecule compounds was done in thermonic apparatus to determine its identity and purity. The observed melting point of isolated compound was compared with the standard melting point of respective gallic acid and quercetin.

#### **7. Antioxidant activity by DPPH method**

2, 2-diphenyl-1-picryl-hydrazyl (DPPH) is a stable organic free radical used to estimate the antioxidant activity of various compounds. The scavenging action on DPPH radical from amla fruit and isolated fraction (FA004 by flash chromatography) and peels of pomegranate extract and isolated fraction (FP004 by flash chromatography of peels of pomegranate extract) determined by following method [38, 39]. As of different concentrations was mixed with an aliquot of DPPH (1 ml, 0.004% w/v) and analyzed at 517 nm. Then the scavenging capacity was calculated using equation number (1).

$$\text{Scavenging activity} \left( \% \right) = \frac{\left( \Delta \text{A517 of control} - \Delta \text{A517 of sample} \right)}{\Delta \text{A517 of control}} \times 100 \tag{1}$$
