**12. Conclusion**

In conclusion, the presence of phenolic compound (gallic acid) and flavonoid (quercetin) could be one of the contributing factors for mechanism of *in vitro* studies on HCT 116 cell lines. Model hydrophobic biomolecules with nanoparticle size range, positive charge on particle with good value and sustained in-vitro releases of gallic acid and quercetin especially in wide pH range of entire gastrointestinal tract from nanoparticles were special findings associated with colonic site. Therefore, discovery and development of new nanoformulation based on natural products have been the reported to have a controlled effect on cancer cell lines; therefore, they have the potential to be used as an important therapeutic anticancer biomolecules. Further studies are warranted to decipher the probable mechanism by which gallic acid and quercetin nanoparticles exert anticancer effect.
