**4. Conclusions**

The work confirms that lutein extract from marigold flower (*T. erecta* L.) had strong an antioxidant potential. The three protocols used i.e. DPPH, ABTS and FRAP provide a good selection method to use for antioxidant measurements. Determination of antioxidant lutein extract will use to developed new drugs in pharmaceutical fields. The results of *in vivo* experiments showed that Lutein extract was able to increase the activity and phagocytic capacity of *S. aureus*-induced rat peritoneal macrophages. Phagocytic processes in immune cells contribute in particular to homeostasis and defense against disease.

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*aureus.*

*aureus.*

*aureus.*

*aureus.*

IV.Group mice were given the extract lutein 0.15 mg/20 g bw/day +0.5 mL *S.* 

V.Group mice were given the extract lutein 0.30 mg/20 g bw/day +0.5 mL *S.* 

VI.Group mice were given the extract lutein 0.60 mg/20 g bw/day +0.5 mL *S.* 

VII.Group mice were given the extract lutein 0.90 mg/20 g bw/day+0.5 mL *S.* 

The results of the Duncan Multiple Range Test on the value of phagocytosis activity for each test group showed that there was no significant difference between the negative control group and the 0.15 mg/20gBW lutein extract treatment group. Treatment of lutein extract 0.30 mg/20gBW, was not significantly different from treatment of lutein extract 0.60 mg/20gBW, while lutein treatment 0.90 mg/20gBW showed a significant difference. The normal control group and the positive control group showed significantly-different phagocytic activity. The lowest phagocytosis activity was found in the normal control group because in the normal control group mice were not given intra-peritoneal *S. aureus* suspension; so, macrophages were not triggered to perform phagocytosis. Macrophages are activated by various stimuli originating from exogenous antigens and microorganisms.

*Histogram of activity and capacity of phagocytosis macrophage cells in mice peritoneum liquids on each test* 

**No. Test Group Phagocytosis Activity (%) Phagocytosis Capacity** 

*Phagocytosis activity of macrophage cell (per 100 cells) and phagocytosis capacity (the number of bacteria* 

I Normal Control 14.25a

II Negative Control 43.50b

III Positive Control 77.75e

IV Lutein Extract 0.15 mg/20 g bw 45.50b

V Lutein Extract 0.30 mg/20 g bw 54.75c

VI Lutein Extract 0.60 mg/20 g bw 57.50c

VII Lutein Extract 0.90 mg/20 g bw 67.00d

*devoured per 50 active macrophage cells).*

*Note: The numbers followed by the same letter are not significantly different (P < 0.05).*

*S. aureus* **cells/ 50 macrophage cells**

± 6.61

± 5.09

± 15.06

± 3.59

± 5.12

± 4.57

± 6.50

± 2.22 46.50a

± 2.65 268.00b

± 4.11 1041.50c

± 1.29 354.75d

± 4.35 519.25e

± 2.65 611.25f

± 2.58 766.75g

**188**

**Table 4.**

**Figure 6.**

*group.*
