*7.2.1 Treatment with ozonated water*

An irrigation and sprinkler system with ozonated water to promote the growth of sugarcane is shown in **Figures 9** and **10**. The fungicide was reduced progressively to control and eliminate fungus and pests as the amount of ozonated water increased. Previously, 90 buds were submerged in ozonated water during 24 h. Thirty buds per variety were immersed in ozonated water (1 ppm) for 10, 20, and 30 min respectively.

## *7.2.2 Witness without treatment*

Thirty buds per variety were soaked on water for 24 h without applying any treatment.

**Figure 9.** *Ozonated water irrigation system as applied on sugarcane cultivation.*

**83**

**Figure 11.**

*Influence of ozonated water in sprouting of sugarcane agamic seed.*

*Innovation in Food Products Using Ozone Technology: Impact on Quality Assurance*

Thirty buds per variety were submerged 24 h in water, then immersed in hot water at 51 °C for one hour. Immediately they were treated with propiconazole (Tilt EC 250) at 0.4% (w/w) for 15 min. This time was considered enough to eliminate

This treatment started on July 4 2008 with the immersion in water of 30 sugarcane buds per variety for 24 h, and then submerged for 30 min in a water solution of Nemacid (EDOCA, Amealco, Querétaro) at 2% (w/w). This organic insecticide is used in the control and inhibition of nemathodes. Quivican experimental station was used to plant the sprouted seeds treated with Nemacid. After 30 days, the sprouting count was carried out and analyzed in the laboratory. All the experiments

All the treatments showed significant differences (p < 0.0001). Best results obtained on seeds immersed in ozonated water for different time intervals are shown in **Figure 11**. The sprouted seeds treated with 10 min-ozonated water immersion reached the highest germination level: 10 seeds in total. The sample control seeds and seeds treated with 30 min-ozonated water reached an acceptable germination rate: 9 and 8 respectively. Nemacid and hot water samples presented the lowest germination rate: 7 in total. Using ozonated water did not affect the viability of the seed. The experimental ozonated water immersion system proposed to producers of sugarcane reduced drastically eyespot and leaf scald. This treatment was beneficial for agamic seed viability showing a different response in every treatment, especially that with 10-min

*DOI: http://dx.doi.org/10.5772/intechopen.96681*

*7.2.3 Hydrothermal + chemical treatment*

leaf scald and red rot diseases.

*7.2.4 Biological treatment*

were made by duplicate.

immersion time.

**7.3 Results and discussion**

**Figure 10.** *Ozonized water by foliar spraying.*

*Innovation in Food Products Using Ozone Technology: Impact on Quality Assurance DOI: http://dx.doi.org/10.5772/intechopen.96681*

#### *7.2.3 Hydrothermal + chemical treatment*

Thirty buds per variety were submerged 24 h in water, then immersed in hot water at 51 °C for one hour. Immediately they were treated with propiconazole (Tilt EC 250) at 0.4% (w/w) for 15 min. This time was considered enough to eliminate leaf scald and red rot diseases.

#### *7.2.4 Biological treatment*

*Innovation in the Food Sector Through the Valorization of Food and Agro-Food By-Products*

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**Figure 10.**

*Ozonized water by foliar spraying.*

**Figure 9.**

*Ozonated water irrigation system as applied on sugarcane cultivation.*

This treatment started on July 4 2008 with the immersion in water of 30 sugarcane buds per variety for 24 h, and then submerged for 30 min in a water solution of Nemacid (EDOCA, Amealco, Querétaro) at 2% (w/w). This organic insecticide is used in the control and inhibition of nemathodes. Quivican experimental station was used to plant the sprouted seeds treated with Nemacid. After 30 days, the sprouting count was carried out and analyzed in the laboratory. All the experiments were made by duplicate.

#### **7.3 Results and discussion**

All the treatments showed significant differences (p < 0.0001). Best results obtained on seeds immersed in ozonated water for different time intervals are shown in **Figure 11**. The sprouted seeds treated with 10 min-ozonated water immersion reached the highest germination level: 10 seeds in total. The sample control seeds and seeds treated with 30 min-ozonated water reached an acceptable germination rate: 9 and 8 respectively. Nemacid and hot water samples presented the lowest germination rate: 7 in total. Using ozonated water did not affect the viability of the seed. The experimental ozonated water immersion system proposed to producers of sugarcane reduced drastically eyespot and leaf scald. This treatment was beneficial for agamic seed viability showing a different response in every treatment, especially that with 10-min immersion time.

**Figure 11.** *Influence of ozonated water in sprouting of sugarcane agamic seed.*
