**2.9 Applications**


#### **2.10 Fluorescence intensity distribution analysis (FIDA)**

The FIDA technique involves the monitoring of the sample's fluorescence intensity with a heterogeneous brightness profile. Hence, it help to determine the concentrations and specific brightness values of a number of individual fluorescent species in a solution [15].

#### **2.11 Nuclear magnetic resonance (NMR)**

Nuclear magnetic resonance based screening is an effective method to help identify leads of low molecular weight organic compound which bind to the protein targets. The screening method provides information on the binding location and affinity of potential lead compounds. This method is especially useful in the designing of highly selective lead structures or when hallow binding pockets need to be targeted. However, the technique is extremely sensitive toward loose fragment binders offering comparatively high throughput.

This Nuclear magnetic resonance NMR based screening method not only provides information of the binding site or the conformation of the bound ligand but it also reveals information related with docking of the ligand to the protein's binding pocket [15].

*Design and Implementation of High Throughput Screening Assays for Drug Discoveries DOI: http://dx.doi.org/10.5772/intechopen.98733*
