Meet the editor

Xianquan Zhan received his MD and Ph.D. in Preventive Medicine from the West China University of Medical Sciences. He received his post-doctoral training in oncology and cancer proteomics at Central South University, China, and the University of Tennessee Health Science Center (UTHSC), USA. He worked at UTHSC and the Cleveland Clinic during 2001-2012, and achieved the rank of associate professor at UTHSC. Currently, he

is a full professor at Central South University and Shandong First Medical University, as well as an advisor to MS/Ph.D. graduate students and postdoctoral fellows. Dr. Zhan is a fellow of the Royal Society of Medicine and the European Association for Predictive, Preventive & Personalized Medicine (EPMA), a national representative for EPMA, and a member of the American Society of Clinical Oncology (ASCO) and the American Association for the Advancement of Sciences (AAAS). He is editor in chief of the *International Journal of Chronic Diseases & Therapy*, associate editor of *EPMA Journal* and *BMC Medical Genomics*, and guest editor of *Frontiers in Endocrinology*, *Mass Spectrometry Reviews*, *EPMA Journal*, and *Oxidative Medicine and Cellular Longevity*. He has published more than 140 articles, twenty-five book chapters, five books, and two US patents in the field of clinical proteomics and biomarkers.

Contents

Introductory Chapter: Metabolomics

*and Gerson-Dirceu López*

*and Mariasingarayan Yosuva*

Drug Discovery

Interferon-Gamma

during Ontogenesis

**Preface XI**

**Chapter 1 1**

**Chapter 2 15**

**Chapter 3 31**

**Chapter 4 67**

**Chapter 5 91**

**Chapter 6 117**

**Chapter 7 137**

Metabolic Profiling of Transgenic Tobacco Plants Synthesizing Bovine

*by Vladislav V. Yemelyanov, Roman K. Puzanskiy, Mikhail S. Burlakovskiy,* 

*by Katerina V. Sazanova, Nadezhda V. Psurtseva and Alexey L. Shavarda*

*by Xianquan Zhan, Jingru Yang, Shu Zheng, Nannan Li and Na Li*

Pharmacometabolomics: A New Horizon in Personalized Medicine

*Hiba Asfour, Chung-ke Chang, Joanna Lachowicz and Mariusz Jaremko*

From Targeted Quantification to Untargeted Metabolomics *by Veronica Lelli, Antonio Belardo and Anna Maria Timperio*

*by Abdul-Hamid Emwas, Kacper Szczepski, Ryan T. McKay,* 

Volatilomics of Natural Products: Whispers from Nature *by Chiara Carazzone, Julie P.G. Rodríguez, Mabel Gonzalez* 

Seagrass Metabolomics: A New Insight towards Marine Based

Metabolomic Changes in Wood Inhabiting Filamentous Fungi

*by Danaraj Jeyapragash, Ayyappan Saravanakumar* 

*Lyudmila A. Lutova and Maria F. Shishova*

## Contents


Preface

The center of multiomics is moving from genome-centered studies to phenomecentered studies in life sciences and medical sciences. Metabolomics, as the important aspect of phenomics, is the methodology and theory for studying the metabolome, including identifying its biochemical and molecular characteristics, characterizing interactions among different metabolites or between metabolites and genetic/environmental factors, and evaluating biochemical mechanisms related to given conditions such as different pathophysiological processes. The metabolome contains all metabolites derived from sugars, lipids, proteins, and nucleic acids in a given biological system, tissue, cell, or body fluid. The metabolites in a metabolome interact mutually in enzymatic reaction systems to form metabolic network systems. Alteration in the metabolome is associated with multiple factors, including genetic, environmental, internal, external, drug, or dietary factors. Currently, the studies on variations in the metabolome are insufficient. Metabolomics includes a targeted approach based on selected/multiple reaction monitoring (SRM/MRM) and an untargeted approach based on nuclear magnetic resonance (NMR) or mass spectrometry (MS). The development of high-throughput, high-sensitivity, and especially high-reproducibility approaches is necessary to maximize the coverage of variations in the metabolome. Studies on metabolomic variations result in the discovery of effective biomarkers to clarify molecular mechanisms of a disease and determine reliable therapeutic targets. These studies also benefit precise prediction, diagnosis, and prognostic assessment in the context of predictive, preventive, and

personalized (3P) medicine, as well as contribute to life sciences.

during ontogenesis.

This book focuses on the advancements in the concept and methodology of metabolomics, including sample preparation, targeted metabolomics based on SRM/MRM, untargeted metabolomics based on NMR or MS, and applications of metabolomics in the research and practice of medical sciences and life sciences. Chapter 1 addresses the concept and importance of metabolomics and its methodology, including targeted and untargeted approaches. Chapter 2 addresses the versatility of metabolomics analysis due to the enormous variety of samples and the absence of strict barriers between quantitative and qualitative analyses. The chapter also highlights untargeted metabolomics using the metabolomics of opposite Antarctic cryptoendolytic communities as an example, and targeted metabolomics using urine during childbirth as an example. Chapter 3 suggests that pharmacometabolomics is a new horizon in personalized medicine, highlighting potentials and advantages in designing innovative and personalized drug treatment. Chapter 4 examines the theory and methodology of volatiomics of natural products from plants, flowers, fruits, microorganisms, and animals. Chapter 5 presents new insights into seagrass metabolomics in marine-based drug discovery through the study of its bioactive products. Chapter 6 discusses the metabolic profiling of transgenic tobacco plants that synthesize bovine interferon-gamma for optimizing the quality and quantity of plant-synthesized interferon-gamma. Chapter 7 discusses the use of gas chromatography (GC)-MS-based metabolomic changes in wood-inhabiting filamentous fungi
