**2. A workflow for intact glycopeptide identification**

A common strategy to determine protein glycosylation is shown in **Figure 2**. This process generally involves: (i) protease treatment of protein(s) to generate a peptide/glycopeptide mixture, (ii) glycopeptide enrichment, (iii) analysis of isolated glycopeptides by LC–MS/MS, (iv) computational analysis of mass data

**Figure 2.** *Basic workflow of intact glycopeptide identification.*

against proteome and glycan reference databases to yield both the peptide sequence and possible glycan structure for each peptide. Here we review technical challenges and recent advances for each step of glycopeptide analysis.
