**10. Quantification of the sum of xanthones mangiferin by UV spectrophotometry**

The content of the sum of xanthones in the test subjects in terms of mangiferin was calculated in two ways, using the optical density of the solution of the standard sample of mangiferin and the value of the specific absorption index of mangiferin under similar conditions. *Determination of specific value of mangiferin uptake:* A precise suspension of a standard mangiferin sample (about 0.01 g) was placed in a measuring flask with a capacity of 25 ml, 20 ml of 70% ethyl alcohol was added, stirred until the standard sample was completely dissolved, and the volume of solution was adjusted to a mark in the flask. Aliquots from the resulting solution were placed in measuring flasks with a capacity of 25 ml and labeled with the same solvent. The optical density was measured on a spectrophotometer at a wavelength


 **3.** *Results of chromatography of* Hedysarum *L. species together with the standard mangiferin (Sigma-Aldrich).* of 365 nm in the resulting solutions. Ethyl alcohol 70% was used as a comparison

The obtained results were statistically processed; the relative error of determination was 1.4%, which makes it possible to conclude the reliability of the obtained results. The optical density of solution B was measured on a spectrophotometer at a wavelength of 365 � 2 nm in a cuvette with a layer thickness of 10 mm. As the comparison solution, 70% ethyl alcohol was used. The quantitative content of the sum of xanthones in terms of mangiferin was calculated using the optical density value of the standard sample of mangiferin(I) and the value of the specific absorption index of mangiferin(II) established by us experimentally. **Table 5** shows the results of quantification of the sum of xanthones in terms of mangiferin in the raw

As a result of complex chromatographic studies of three species of the genus *Hedysarum*, it was revealed that the maximum content of the sum of xanthones in

*Hedysarum* (**Table 6**). The obtained results indicate the prospect of further study of the above-ground part, which allows us to consider this species as an additional raw

0.25 4 0.1278 319.5 *Sx*=18,433

**Calculated value of specific key figure absorption,** *А***1%**

> *А*1% <sup>1</sup>*см*=325.1

**Maintenance of the sum of xanthones, % (***А***1%**

**<sup>1</sup>***cm***)**

**I II I II**

**1***см*

**Metrological characteristics**

> *ΔX* ¼ 4*:*52 *t*0,95 ¼ 2*:*45 325*:*1 � 4*:*52 *ε* ¼ 1*:*4%

**Metrological characteristics**

<sup>1</sup>см ¼*325.1;*

terms of mangiferin is 0.62 � 0.021%, and this is observed in the grass of

**Optical density,** *А*

*Results of determination of specific value of mangiferin absorption (exact weight 0.0101 g).*

*Content of the sum of xanthones in terms of mangiferin (а<sup>0</sup> = 0.0101 г; A0 = 0.5178;* А<sup>1</sup>%

10.015 0.4621 0.640 0.637 *<sup>X</sup>*´ <sup>¼</sup>0.629 *<sup>X</sup>*´ <sup>¼</sup> 0.624 10.018 0.4672 0.647 0.644 *Sx*´ ¼0.0830 *Sx*´ ¼0.0830 10.100 0.4671 0.642 0.639 *Δx* ¼0.021 *Δx* ¼0.021 0.9989 0.4329 0.601 0.599 *x*´ � *Δx* ¼ *x*´ � *Δx* ¼ 0.9996 0.4358 0.605 0.602 0.629 � 0.021 0.624 � 0.021 0.9898 0.4504 0.632 0.629 *ε* ¼3.39% *ε* ¼3.42%

**Maintenance of the sum of xanthones, % (А0)**

0.50 8 0.2603 325.4 0.75 12 0.3945 328.8 1.00 16 0.5178 325.7 1.25 20 0.6427 321.4 1.50 24 0.7718 321.5 1.75 28 0.9338 333.5

solution. **Tables 4** and **5** shows the results of the experiment.

material (**Table 5**).

**Aliquot, ml**

**Table 4.**

**Table 5.**

**159**

*w = 7.23%).*

**Mass, g Value of optical density, Ах (λ = 364 nm)**

material source of mangiferin.

Hedysarum *Species from Caucasus*

*DOI: http://dx.doi.org/10.5772/intechopen.91741*

**Concentration of mangiferin, %** �**10**�**<sup>4</sup>**

#### Hedysarum *Species from Caucasus DOI: http://dx.doi.org/10.5772/intechopen.91741*

of 365 nm in the resulting solutions. Ethyl alcohol 70% was used as a comparison solution. **Tables 4** and **5** shows the results of the experiment.

The obtained results were statistically processed; the relative error of determination was 1.4%, which makes it possible to conclude the reliability of the obtained results. The optical density of solution B was measured on a spectrophotometer at a wavelength of 365 � 2 nm in a cuvette with a layer thickness of 10 mm. As the comparison solution, 70% ethyl alcohol was used. The quantitative content of the sum of xanthones in terms of mangiferin was calculated using the optical density value of the standard sample of mangiferin(I) and the value of the specific absorption index of mangiferin(II) established by us experimentally. **Table 5** shows the results of quantification of the sum of xanthones in terms of mangiferin in the raw material (**Table 5**).

As a result of complex chromatographic studies of three species of the genus *Hedysarum*, it was revealed that the maximum content of the sum of xanthones in terms of mangiferin is 0.62 � 0.021%, and this is observed in the grass of *Hedysarum* (**Table 6**). The obtained results indicate the prospect of further study of the above-ground part, which allows us to consider this species as an additional raw material source of mangiferin.


#### **Table 4.**

*Results of determination of specific value of mangiferin absorption (exact weight 0.0101 g).*


#### **Table 5.**

**Value**

**158**

**Color of** 

**chromatographic**

 **area** *H.* 

*grandiflorum* **Pall.**

*H.*  **Boiss.**

*daghestanicum* **Rupr. ex**

**Standard mangiferin**

**Aldrich)**

 **(Sigma-**

**Alpizarin**

*Legume Crops - Prospects, Production and Uses*

*Rf*

*H. caucasicum* **M. Bieb.**

**Before**

**After**

**Before**

**After**

**Before**

**After**

**Before**

**After**

**Before**

**After**

**detecting**

System 1:

0.51

System 2:

0.56

System 3: acetic acid 15%

0.38

**Table 3.** *Results of* 

*chromatography*

 *of*

Hedysarum *L. species together with the standard mangiferin*

 Pale yellow

 Bright yellow

 Pale yellow

 Bright yellow

 Pale yellow

*(Sigma-Aldrich).*

 Bright yellow

 Pale yellow

 Bright yellow

 Pale yellow

 Orange

 Pale yellow

 Bright green

 Pale yellow

 Bright green

 Pale yellow

 Bright green

 Pale yellow

 Bright green

 Pale yellow

 Bright green

chloroform-methanol-water

 (13:7:2)

 Pale yellow

 Bright yellow

 Pale yellow

 Bright yellow

 Pale yellow

 Bright yellow

 Pale yellow

 Bright yellow

 Pale yellow

 Bright yellow

n-butanol-acetic

 acid-water (4:1:5)

**detecting**

**detecting**

**detecting**

**detecting**

**detecting**

**detecting**

**detecting**

**detecting**

**detecting**

*Content of the sum of xanthones in terms of mangiferin (а<sup>0</sup> = 0.0101 г; A0 = 0.5178;* А<sup>1</sup>% <sup>1</sup>см ¼*325.1; w = 7.23%).*


**Table 6.**

*The content of the sum of xanthones in terms of mangiferin in the above-ground part of species of the genus* Hedysarum *L. by the value of the specific absorption index of mangiferin.*
