Hedysarum *Species from Caucasus DOI: http://dx.doi.org/10.5772/intechopen.91741*

**Figure 8.** *Chromatogram of standard solution of mangiferin.*

**Figure 9.** *Chromatogram of solution 1.*

which confirms the assumption based on molecular genetic studies, since it is this species that belongs to the Obscura section, as well as the alpine penny used to

**12. Quantification of mangiferin in the grass of** *Hedysarum caucasicum*

High-performance liquid chromatography was used to quantify mangiferin in the subjects under study. Registration of electronic spectra was carried out on a liquid chromatograph Shimadzu Prominence LC-20 AD with a degasser DGU-20A3R. A sample of mangiferin (Sigma-Aldrich, cate. M3547, USA, 2017) was used

*Standard mangiferin solution*: A slurry of 0.0050 g (based on 100% substance) of mangiferin was placed in a measuring flask with a capacity of 25 ml, dissolved in 70% ethyl alcohol, and then the volume was adjusted with the same solvent to a mark and mixed. An aliquot of 1.0 ml of the resulting solution was placed in a measuring flask with a capacity of 10 ml, dissolved in the mobile phase, and then the volume was adjusted with the same solvent to a mark and stirred. The solution

*Buffer solution*: In a 1000 mL measuring flask, 15.6 g of sodium phosphate of monosubstituted dihydrate was placed, which was dissolved with 200 mL of purified water and the volume adjusted to a mark, stirred. The potentiometric method

*Mobile phase*: A mixture of buffer solution, acetonitrile, and methanol in a ratio

The developed method of quantitative determination of mangiferin in the above-ground organs of the studied type takes into account the main physicochemical properties of xanthones, and it is characterized by reproducibility, high accuracy, and allows conducting both screening assessment of various raw materials containing mangiferin derivatives and standardization of prepared medicinal plant

As a result, it was found that the largest in the above-ground organs of *Hedysarum caucasicum* found was 0.148 0.003% mangiferin (**Figures 9** and **10**). The method of quantification of mangiferin by HPLC has been developed, which is

was filtered through a 0.45 μm membrane filter (**Figure 8**).

*Electrophoregram of extraction of* Hedysarum daghestanicum *Rupr. ex Boiss.*

*Legume Crops - Prospects, Production and Uses*

determined the value of pH, which should be 4.40 0.05.

obtain mangiferin.

**Figure 7.**

**M. Bieb. by HPLC**

as a standard sample.

of 81:16:3.

**162**

raw materials of the *Hedysarum* L.

**Figure 10.** *Chromatogram of solution 2.*

characterized by good reproducibility and tested at raw materials. The obtained results can be used in the preparation of regulatory documentation for plant raw materials. As a result, in species *Hedysarum caucasicum* M. Bieb., *Hedysarum grandiflorum* Pall., and *Hedysarum daghestanicum* Rupr. ex Boiss., biologically active substances such as polysaccharides, triterpene compounds, flavonoids, saponins, polyphenol compounds, leucoanthocyanins, and tannins were found. The quantitative content of tannins in *Hedysarum caucasicum M. Bieb*. was 5.43 0.15%.
