**Abstract**

Every year approximately 30–50% of crops suffer with different kinds of biotic stresses. Rapidly growing agrochemical industries and their diverse products make the environment more toxic and simultaneously hazardous for plant heath and soil health. Such types of agrochemicals are toxic, hazardous, carcinogenic, non-ecofriendly. Therefore, this is the ideal time to think about some more effective alternatives against those problems. Nanotechnological approaches bring the alternatives in the form of decreasing toxicity, improving shelf-life, increasing solubility for poorly water-soluble agrochemicals, minimum use with maximum effect, slow leaching efficiency with long-term effect with coupling of eco-friendly naturalistic way. The way of nanoparticle application in agriculture, specifically disease management, is unique, where it can be used singly or by coupling with fungicidal, herbicidal, insecticidal, RNA-interference molecules. Though it has such a positive impact, very few products will be commercially available in our market due to high price of particular products and well-established long field trial efficacy detection among insect, pest-pathogen, and environment. Application of nanomolecules in other progressive fields has been emerging, whereas advancement in agricultural applications needs to be boosted up through skilled knowledge transfer and basic understanding of its fundamental aspect.

**Keywords:** nanotechnology, IDM, sustainable, eco-friendly

### **1. Introduction**

The population of the world is increasing in an alarming rate. The global demand for food production will need to double by 2050. But, the climate change such as prolonged drought, sudden increase of temperature, unpredictable rains, and floods are the main barrier to achieve this global food demand [1]. Moreover, significant crop losses, that is, 20–40% per year due to some biotic causes is another major problem in worldwide basis [2]. The biotic causes mostly include insects, nematodes, pathogens, weeds, human, and animal interaction. Earlier studies revealed that approximately 20 and 26% yield loss occurred due to pathogen and insect infestation [3]. Most developed and developing countries depend upon chemical pesticides to control the diseases and pest incidence as these are easily available and show the result quickly. Industrial business and marketing policy also sometimes cross their barrier to gain some profit. Therefore human made behavioristic alteration of our environment also considered as a crucial responsible factor.

But they do not think those chemicals are harmful to the ecosystem, poisonous to beneficial insects, create pest resurgence, 90% loss of chemical during or post application in field leading to more economical loss for farmers. By keeping all the aforesaid reality based problem in mind, a new potential concept and technique known as nanotechnology is developed by the scientists, which is cost-effective, reliable, eco-friendly, and very effective. Although, this concept has not gained more focus in agriculture compared to pharmacology and medicine, but still it takes part a major role in plant breeding, nano sensors, plant hormone delivery, limited application of chemicals, plant health management etc. [4–6].

Nanotechnology refers to the technology related to application and manipulation of nanoparticles, that is, very small particles or materials having one or more dimensions, that is, 1–100 nm and fashioned with exclusive physical, chemical, and biological properties [7]. Several scientists have worked on the desired characteristics of nanoparticles such as pore shape, size etc. for accurate and specific application through adsorption or encapsulation of effective pesticide [8]. Application of nanoparticles can be functionalized in two ways, that is, first, nanoparticles directly involves as a plant protectant and second, nanoparticles used as carrier. On the first way researchers have tested the viability of the nanomolecules and mode of action against a particular pathogen. On the second way nanoparticles used with the existing fungicides or pesticide, herbicides, RNAi-mediated coupled component, to boost its activity. Nanoparticles used as a carrier have some positive sites like it can increase solubility of the coupled component, increase shelf-life, boosting site- and target-specific activity, reduce toxicity level, and maintain environmental safety. The implementation and utilization of fruits of nanotechnological applications, in terms of plant disease detection and management, gene editing and transformation is at infancy stage till now due to insufficiency of knowledge and skills. In the present context, the use of nanotechnology in plant disease management and allied sectors of agriculture is a real challenging task, which is objectified and framed with the present chapter.

Context of sustainable agriculture and nanotechnology


**15**

*Nanotechnological Approaches in Sustainable Agriculture and Plant Disease Management*

to stabilize soil horizons in addition to lessening the ecotoxicity. Nanoparticles or nanotubes are found to be effective in retaining the water inside the hollow core for longer period by modifying the xylem vessel mechanism and metabolism of plant cells. Due to the partial solubility and dispersible characteristics, the water is retained inside plant cells for more time. In 2009, Corredor et al. demonstrated carbon nanotube treatment in pumpkin plant to analyze the water retention capacity and observed the accumulated carbon nanotubes inside the plant cell, which act as a water transporter. Due to the cohesive force, the water moves continuously through the nanotubes in plant cell forming a

4.**Significance in the field of plant disease management:** Recent days, nanotechnology is taking part a major role in plant disease management due to its ecofriendly nature and potentiality. Among all nanobased particles, silver nanoparticles stand out in the frontline in plant defense. These nanoparticles disturb the cell DNA, metabolic activity, electron transport chain, nutrient uptake of micro-organisms leading to death. The pathogenic fungi which can be controlled by using silver nanoparticles are *Colletotrichum gloeosporioides, Alternaria solani, Fusarium oxysporum, F. solani, Macrophomina phaseolina, Rhizoctonia solani, Aspergillus niger* etc. Silica-silver based nanoparticles are reported to inhibit the growth of bacteria *Pseudomonas syringae, Xanthomonas campestris* pv*. vesicatoria* up to 100%. Copper-based nanoparticles were found to be effective against bacteria *Xanthomonas oryzae*pv*. Oryzae*, *Xanthomonas campestris* pv*. phaseoli* and

fungi *Fusarium solani, Alternaria solani, Aspergillus flavus* etc. [12, 13].

of these two modes are shown under **Figure 1**.

uptake as compared to conventional chemicals.

virus when applied after infection [19].

A. **Limitations of different applied disease management strategies:**

Drawbacks or limitation of all applied disease management practices are

I.**Nanoparticles used as protectants:** Potential nanoparticles used alone to protect the plants from pathogenic micro-organisms and applied directly to plant and plant parts. Several metal nanoparticles like copper, silver, zinc oxide, titanium dioxide is experimented for their antagonistic effects against all pathogenic bacteria, virus, fungus, and concluded as successful potential protectants [14–16]. These nanoparticles have more shelf life, easily soluble in water, and show site specific

a.**Silver nanoparticle:** It is in the frontline due to its "green synthesis" production mechanism in bacteria, yeast, fungi, and plants [17]. From previous studies, it is reported that silver nanoparticles have antifungal effect against *Alternaria alternata, Sclerotinia sclerotiorum, Macrophomina phaseolina, Botrytis cinerea, Rhizoctonia solani,* etc. by well diffusion assay [18] while antiviral effect on sun hemp rosette virus when sprayed on leaves and bean yellow mosaic

B. **Types of nanoparticles for plant disease management:** Nanoparticles can be used to manage the plant disease through two different mechanisms: as protectants where the nanoparticles only protect the plants and as carrier where the nanoparticles contain potential pesticides or some other active compounds [2]. Schematic representations of mode of action

*DOI: http://dx.doi.org/10.5772/intechopen.92463*

nanosized water stream [11].

depicted under **Table 1**.

to stabilize soil horizons in addition to lessening the ecotoxicity. Nanoparticles or nanotubes are found to be effective in retaining the water inside the hollow core for longer period by modifying the xylem vessel mechanism and metabolism of plant cells. Due to the partial solubility and dispersible characteristics, the water is retained inside plant cells for more time. In 2009, Corredor et al. demonstrated carbon nanotube treatment in pumpkin plant to analyze the water retention capacity and observed the accumulated carbon nanotubes inside the plant cell, which act as a water transporter. Due to the cohesive force, the water moves continuously through the nanotubes in plant cell forming a nanosized water stream [11].

	- A. **Limitations of different applied disease management strategies:** Drawbacks or limitation of all applied disease management practices are depicted under **Table 1**.
	- B. **Types of nanoparticles for plant disease management:** Nanoparticles can be used to manage the plant disease through two different mechanisms: as protectants where the nanoparticles only protect the plants and as carrier where the nanoparticles contain potential pesticides or some other active compounds [2]. Schematic representations of mode of action of these two modes are shown under **Figure 1**.
		- I.**Nanoparticles used as protectants:** Potential nanoparticles used alone to protect the plants from pathogenic micro-organisms and applied directly to plant and plant parts. Several metal nanoparticles like copper, silver, zinc oxide, titanium dioxide is experimented for their antagonistic effects against all pathogenic bacteria, virus, fungus, and concluded as successful potential protectants [14–16]. These nanoparticles have more shelf life, easily soluble in water, and show site specific uptake as compared to conventional chemicals.
			- a.**Silver nanoparticle:** It is in the frontline due to its "green synthesis" production mechanism in bacteria, yeast, fungi, and plants [17]. From previous studies, it is reported that silver nanoparticles have antifungal effect against *Alternaria alternata, Sclerotinia sclerotiorum, Macrophomina phaseolina, Botrytis cinerea, Rhizoctonia solani,* etc. by well diffusion assay [18] while antiviral effect on sun hemp rosette virus when sprayed on leaves and bean yellow mosaic virus when applied after infection [19].

*Organic Agriculture*

But they do not think those chemicals are harmful to the ecosystem, poisonous to beneficial insects, create pest resurgence, 90% loss of chemical during or post application in field leading to more economical loss for farmers. By keeping all the aforesaid reality based problem in mind, a new potential concept and technique known as nanotechnology is developed by the scientists, which is cost-effective, reliable, eco-friendly, and very effective. Although, this concept has not gained more focus in agriculture compared to pharmacology and medicine, but still it takes part a major role in plant breeding, nano sensors, plant hormone delivery, limited

Nanotechnology refers to the technology related to application and manipulation of nanoparticles, that is, very small particles or materials having one or more dimensions, that is, 1–100 nm and fashioned with exclusive physical, chemical, and biological properties [7]. Several scientists have worked on the desired characteristics

of nanoparticles such as pore shape, size etc. for accurate and specific application through adsorption or encapsulation of effective pesticide [8]. Application of nanoparticles can be functionalized in two ways, that is, first, nanoparticles directly involves as a plant protectant and second, nanoparticles used as carrier. On the first way researchers have tested the viability of the nanomolecules and mode of action against a particular pathogen. On the second way nanoparticles used with the existing fungicides or pesticide, herbicides, RNAi-mediated coupled component, to boost its activity. Nanoparticles used as a carrier have some positive sites like it can increase solubility of the coupled component, increase shelf-life, boosting site- and target-specific activity, reduce toxicity level, and maintain environmental safety. The implementation and utilization of fruits of nanotechnological applications, in terms of plant disease detection and management, gene editing and transformation is at infancy stage till now due to insufficiency of knowledge and skills. In the present context, the use of nanotechnology in plant disease management and allied sectors of agriculture is a real

challenging task, which is objectified and framed with the present chapter.

1.**Application of nanotechnology to increase crop productivity and quality:** A variety of nanoparticles were tested previously to study their efficacy for boosting the yield as well as quality of the crop. It was observed that the nanoparticles modify enzymatic action, electron transport system, and influence the nutrition uptake. Carbon nanoparticles are reported to increase the yield of bitter gourd by enhancing the biomass, water content, fruit weight, length, and number. Lentil seeds show high germination rate and growth when treated with silica-based nanoparticles. Titanium dioxide nanoparticles can boost the water uptake, breakdown of organic compounds, photosynthesis

2.**Increase of photosynthetic efficiency through nanotechnological application:** Seeds incorporated with nanoparticles can promote the photosynthetic activity in plants. The nanoparticles respond to a specific wavelength of light and increase the optical potential of leaves which in result effect the hills reaction. Titanium dioxide-based nanoparticles can enhance the water and nutrient uptake, light absorbance and activity of Rubisco activase enzyme resulting more photosynthetic activity in plants. These nanoparticles induce the plants to be photosynthetically more active, grow faster, resulting in quality food

3.**Improvement in water retention and management:** Nanotechniques can be imposed in agriculture to reduce the loss through evaporation, irrigation, and

Context of sustainable agriculture and nanotechnology

application of chemicals, plant health management etc. [4–6].

**14**

capacity, etc. [9].

production in less period of time [9, 10].


#### **Table 1.**

*Limitations of different disease management prcatices.*


**17**

**Figure 1.**

*Nanotechnological Approaches in Sustainable Agriculture and Plant Disease Management*

II.**Nanoparticles that act as carriers:** Nanoparticles are engineered in such a way that these can carry, encapsulate, or absorb active potential compounds to develop effective agricultural pesticides. These carrier nanoparticle-based pesticides have less toxicity, slow leaching capacity, and highly target specific. The below-mentioned nanoparticles are

*Schematic representation of different nanoparticles and their mode of action in term of carrier and protectant.*

a.**Silica nanoparticles:** These nanoparticles have specific size, shape, and structure making them able to deliver the active compounds in the target site [27]. They are mainly round or circular like structure having a pore like holes, for example, mesoporous silica nanoparticles (MSNs) or porous hollow silica nanoparticles (PHSNs). The active compounds are mainly loaded in the inner hole to keep them protect and proper release in target sites. The outer shell guards the

b.**Nano alumino-silicate**: The major advantages for the use of nano alumino-silicate are that it is biologically active, eco-friendly, and more effective than other nanoparticles. The alumino-silicate nanotube containing active compounds when sprayed on plants, the insects present on plants intake the active compounds contained

active compounds against degradation by UV light.

*DOI: http://dx.doi.org/10.5772/intechopen.92463*

mainly used as carrier:

nanotubes [28].

*Nanotechnological Approaches in Sustainable Agriculture and Plant Disease Management DOI: http://dx.doi.org/10.5772/intechopen.92463*

#### **Figure 1.**

*Organic Agriculture*

b.**Nano carbon:** The carbon nanoparticles not only show antimicrobial activity in plant health management but also positively affect the plant growth. These nanoparticles from graphene oxide sheets have shown antimicrobial activity against *Aspergillus niger, A. oryzae, and Fusarium oxysporum in vitro* [20]. Many researchers have anticipated about the mechanism behind the inhibition of microbial growth by carbon nanomaterials and explained that the nano edges of graphene oxide come in direct contact with the chemical compounds present in cell wall of fungi and bacteria making them

c.**Titanium dioxide nanoparticle:** When this nanoparticle is used as fertilizer was known to show antibacterial, antiviral and insecti-

d.**Poly dispersed gold nanoparticles**: It was reported to inactive the Barley yellow mosaic virus and develop plant resistance [24, 25].

e.**Chitosan nanoparticles** control alfalfa mosaic virus, *Fusarium* spp*., Botrytis* spp*., Pyriularia grisea* while show limited effect against bacteria [26]. The mechanisms behind the antimicrobial properties of chitosan are inhibition of the growth of pathogen, protein synthesis, and ATPase activity, agglutination, interruption of cell membrane, disruption of nutrient flow etc. Chitosan nanoparticles have also been reported to control aphids, root knot

inactive [21–23].

*Limitations of different disease management prcatices.*

cidal characteristics.

nematode, and cotton leaf worm.

**16**

**Table 1.**

*Schematic representation of different nanoparticles and their mode of action in term of carrier and protectant.*

	- a.**Silica nanoparticles:** These nanoparticles have specific size, shape, and structure making them able to deliver the active compounds in the target site [27]. They are mainly round or circular like structure having a pore like holes, for example, mesoporous silica nanoparticles (MSNs) or porous hollow silica nanoparticles (PHSNs). The active compounds are mainly loaded in the inner hole to keep them protect and proper release in target sites. The outer shell guards the active compounds against degradation by UV light.
	- b.**Nano alumino-silicate**: The major advantages for the use of nano alumino-silicate are that it is biologically active, eco-friendly, and more effective than other nanoparticles. The alumino-silicate nanotube containing active compounds when sprayed on plants, the insects present on plants intake the active compounds contained nanotubes [28].

**19**

**Table 2.**

*Nanotechnological Approaches in Sustainable Agriculture and Plant Disease Management*

tants (herbicide/fungicide/insecticide)

III.Application of nanoparticles to boost/develop commercial agro-protec-

a.**Nanoparticles as carriers for herbicides:** Nanocarrier-based

herbicide studied mainly confined to reduce the environmental toxicity, which occurs due to conventional herbicides. The herbicides, carrier-based nanoparticles with target pest and toxicity is listed in

b.**Nanoparticles as carriers for fungicides:** The mostly used nanoparticle carriers for fungicidal use are silica, chitosan, polymer mixes etc. Nanoparticles for carrier-based fungicides are much popular as they can reduce the loss through volatilization, increase solubility and release chemicals at target sites slowly. The list of nanocarrier-based fungicide, its target pest with crop details, is

c.**Nanoparticles as carriers for insecticides:** Nanoparticles can be used to lower the toxicity level while increasing its solubility in water. Additionally, these particles lessen the volatilization loss resulting effective protection on targeted sites. The nanoparticles as carrier of insecticide, its target and crop details given in the below-

IV.**Application of nanoparticles in gene delivery system and to moderate gene expression:** The nanoparticles can be referred as molecular cargo as they can easily transfer the genetic materials such as nucleic

*DOI: http://dx.doi.org/10.5772/intechopen.92463*

the below **Table 2**.

given in below **Table 3**.

mentioned **Table 4**.

*Nanocarrier-based herbicidal characterization and their target pests [40–46].*

*Nanotechnological Approaches in Sustainable Agriculture and Plant Disease Management DOI: http://dx.doi.org/10.5772/intechopen.92463*

	- a.**Nanoparticles as carriers for herbicides:** Nanocarrier-based herbicide studied mainly confined to reduce the environmental toxicity, which occurs due to conventional herbicides. The herbicides, carrier-based nanoparticles with target pest and toxicity is listed in the below **Table 2**.
	- b.**Nanoparticles as carriers for fungicides:** The mostly used nanoparticle carriers for fungicidal use are silica, chitosan, polymer mixes etc. Nanoparticles for carrier-based fungicides are much popular as they can reduce the loss through volatilization, increase solubility and release chemicals at target sites slowly. The list of nanocarrier-based fungicide, its target pest with crop details, is given in below **Table 3**.
	- c.**Nanoparticles as carriers for insecticides:** Nanoparticles can be used to lower the toxicity level while increasing its solubility in water. Additionally, these particles lessen the volatilization loss resulting effective protection on targeted sites. The nanoparticles as carrier of insecticide, its target and crop details given in the belowmentioned **Table 4**.


**Table 2.** *Nanocarrier-based herbicidal characterization and their target pests [40–46].*

*Organic Agriculture*

c.**Chitosan nanoparticles:** Due to the low solubility in aqueous media, chitosan nanoparticles are applied by mixing with some organic or inorganic or copolymer compounds to increase its solubility [29]. These compounds stick to the stem and leaf epidermis facilitating them for prolonged effect in target sites [27]. Presence of amines and hydroxyl groups, enable the nanoparticles

d.**Nanocopper:** Copper is one of the most effective and potential chemicals in combating wide range of plant diseases. For the first time in 2013, Giannousi et al. studied the potential antimicrobial characteristics of nanocopper particles and concluded that the nanocopper particles are more promising than other copper-based chemicals [31]. From past studies, it was reported to control or minimizes the growth of the pathogen of Fusarium wilt of tomato, *Verticillium* wilt of eggplant, Leaf spot, leaf blight diseases etc. [32, 33]. Biosynthesized nanocopper derived from *Streptomyces griseus* was observed to limit the growth of *Poria hypolateritia* caus-

e.**Nanozinc:** The characteristic antimicrobial properties of

nanozinc particles are mostly studied in vitro condition. It was reported to control bacteria, fungi, for example, *Alternaria alternata, Botrytis cinerea, Sclerotinia sclerotiorum, Rhizopus stolonifera, Rhizoctonia solani, Mucor* spp*., Fusarium oxysporum,* and *Penicillium* spp. [35, 36]. Disease suppression in protected cultivation and green house condition through nanozinc is also

f. **Solid lipid nanoparticles (SLNs):** These nanoparticles contain lipids, which remain in solid state at room temperature. Therefore, the lipophilic compounds show its prolonged effect by slow releasing of active compounds without adding any extra organic solvents [37]. The limitations are that the active compound may escape

g.**Layered double hydroxides (LDHs):** These nanoparticles break down when come in contact with water or carbon dioxide [39]. The positively charged LDH nanoparticles make the active compounds

h.**Nanoemulsions:** Nanoemulsions are combination of more than one liquid that does not mix easily. These nanoparticles contain active ingredients in the droplets of diameter 500 nm or less and lessen the

degradation of chemicals or active ingredients inside [35].

i. **Dendrimers:** These nanoparticles are branched, similar to tree like structure, having a central core, which is occupied by functional groups [1]. Very few reports and research have done on dendrimers related to plant pathology. It can be referred as a great delivery vehicle as it can transport chemicals or pesticides in basipetal manner after a foliar spray. It can enhance the perme-

easier to move through the plant cell wall.

ability in diseased plant cells.

to enhance its properties [30].

ing root rot disease of tea [34].

possible.

during storage [38].

**18**


**Table 3.**

*List of nanocarrier-based fungicides and their application in plant disease management [47–52].*


#### **Table 4.**

*Nano-protectant with insecticidal activity [53–59].*

acids to the target site and induce to express the new genetic material in the recipient. Surface-functionalized vertically arranged carbon nanofibers act as molecular cargo and carry the plasmid DNA to the

**21**

*Nanotechnological Approaches in Sustainable Agriculture and Plant Disease Management*

negative effect by modifying some genes.

desired site, expressing the characters in the recipient similar to other conventional methods. Researchers had developed a fluorescent labeled starch nanoparticle-based transgene vector that is able to enter the plant cell and nuclear membrane to deliver the genetic materials. In this system, ultrasound and fluorescent label help in gene delivery by producing transient pores and visual tracking of transgene respectively. Some nanoparticles carry the genetic material by holding it tightly so that it will not get detached from the nanoparticle thereby expressing for short time without integrating to the genetic material of the recipient. Apart from this, sometimes nanoparticle-based gene transfer show

V.**Nanoparticles used for abiotic stress tolerance:** Nanoparticles are reported to reduce or tolerate abiotic stress like salt stress, heavy metal toxicity, biotic stress etc. In addition to this, nanoparticles can provide mechanical strength to plants, promote germination and seedling growth, help in nutrient and water uptake, etc. Zinc, copper, and iron nanoparticles are observed up to 40 times less lethal than their salts. Hence, these nanoparticles widen the scope for more productivity than other salt-based applications [60]. In 2014, Sabaghnia and Janmohammadi reported that SiO2nanoparticles enhanced germination percentage, shoot and root length, seedling fresh and dry weight in lentil plant [61]. In 2017, Taran et al. concluded that the drought tolerance in winter wheat can be alleviated by inducing higher antioxidant enzyme activity when treated with the colloidal solution of Cu and Zn

a.**Wet chemistry method:** This method is one of the best methods in preparation of silver nanoemulsion as it combines in molecular level and versatile in nature. This method is first time carried out by Guzman et al. in 2009. In this method, silver nitrate, hydrazine hydrate, and sodium dodecyl sulfate act as metal precursor, reducing agent, and stabilizing agent respectively. The creation of silver nanoparticles was observed with the help of UV-Vis absorption spectros-

b.**Ion implantation method:** This method was carried out by Popok and his coworkers in 2005. In this method, silver ions were implanted to synthesize the metal nanoparticles in SiO2 by using 30 KeV energy

compound was done by using optical spectroscopy and atomic force

c.**Physical vapor deposition:** This method was explained by Lin and his coworkers in 2003. In this method, the overall size of silver trifluoroacetate reduced up to 7–11 nm under isoamyl ether and oleic acid solution by properly maintaining the temperature variation. This process is

. The analysis of this new

and ion current density of 4–15 μA/ cm2

widely accepted and very smooth to manage [54].

5.**Nanotechnological applications to reduce postharvest loss:** Nanotechnology can be used to limit the post-harvest loss of solid, liquid or processed food

*DOI: http://dx.doi.org/10.5772/intechopen.92463*

nanoparticles [62].

copy [63].

microscopy [53].

C. Preparation of silver nanoemulsion

desired site, expressing the characters in the recipient similar to other conventional methods. Researchers had developed a fluorescent labeled starch nanoparticle-based transgene vector that is able to enter the plant cell and nuclear membrane to deliver the genetic materials. In this system, ultrasound and fluorescent label help in gene delivery by producing transient pores and visual tracking of transgene respectively. Some nanoparticles carry the genetic material by holding it tightly so that it will not get detached from the nanoparticle thereby expressing for short time without integrating to the genetic material of the recipient. Apart from this, sometimes nanoparticle-based gene transfer show negative effect by modifying some genes.

	- a.**Wet chemistry method:** This method is one of the best methods in preparation of silver nanoemulsion as it combines in molecular level and versatile in nature. This method is first time carried out by Guzman et al. in 2009. In this method, silver nitrate, hydrazine hydrate, and sodium dodecyl sulfate act as metal precursor, reducing agent, and stabilizing agent respectively. The creation of silver nanoparticles was observed with the help of UV-Vis absorption spectroscopy [63].
	- b.**Ion implantation method:** This method was carried out by Popok and his coworkers in 2005. In this method, silver ions were implanted to synthesize the metal nanoparticles in SiO2 by using 30 KeV energy and ion current density of 4–15 μA/ cm2 . The analysis of this new compound was done by using optical spectroscopy and atomic force microscopy [53].
	- c.**Physical vapor deposition:** This method was explained by Lin and his coworkers in 2003. In this method, the overall size of silver trifluoroacetate reduced up to 7–11 nm under isoamyl ether and oleic acid solution by properly maintaining the temperature variation. This process is widely accepted and very smooth to manage [54].

*Organic Agriculture*

**20**

**Table 4.**

**Table 3.**

*Nano-protectant with insecticidal activity [53–59].*

acids to the target site and induce to express the new genetic material in the recipient. Surface-functionalized vertically arranged carbon nanofibers act as molecular cargo and carry the plasmid DNA to the

*List of nanocarrier-based fungicides and their application in plant disease management [47–52].*

items and beverages. A sensory coating is applied on the product through wrapping or encapsulation method, which slightly modifies the color of the product by improving the taste and shelf-life of the food item. Thin layer of nanoparticles protects the food products from spoilage by holding moisture. Some of the nanoparticles proven promising in lessening the post-harvest loss are carbon nanoparticles, zinc oxide etc., which are mainly antibacterial [9].

	- a.**Toxicological and environmental safety concerns:** The more use of nanoparticles can lead to negativity on agriculture as many nanosubstances have proven toxic to some beneficial micro-organisms. The uncontrolled application of nanobased agrochemicals can usher to environmental pollution and disturbances in food chain. Before mass release of any nanoparticles, it should be checked properly for safety protocols of toxic composition and its residual effect [9]. A previous study has reported that the pure aluminum-based nanoparticles inhibit root growth of many plants while aluminum oxide-based nanoparticles act as a pollutant and hamper crop growth [68]. Similarly, nanozinc oxide-based products

**23**

*Nanotechnological Approaches in Sustainable Agriculture and Plant Disease Management*

improve the food quality of soybean while nano-CeO2 has reported to reduce the soybean yield and nitrogen fixing potential [69]. Therefore, the nanobased products should be in controlled use with prior precaution measures and existing regulatory frameworks to lessen the negative effects

b.**Legal and regulatory action:** Nonhomologous legal protocols refer to nano entities. In some developing countries like India, some compounds or particles with known application characteristics, if modified to nanoparticles, it will not be registered under patent. Section 3d of Indian patent act limited or restricted the common or already known materials from patent registration thereby opening the ways to do more research on it [70]. These types of laws have widened the paths to find out the scope of nanotechnological application, which will lead to sustainable agriculture.

c.**Socioeconomic perspective:** The nanotechnological application not only used to get more benefits in case of farmers as well as entrepreneur but also to take our agricultural system toward sustainability. All the nanoproducts should be properly labeled, which will make the farmers to choose the required products. Nanoparticles and nanofibers can improve the efficacy of agrochemicals in less period of time. The use of natural occurring nano formulations is very old. The potentiality of these formulations is very large, which can be known from the patents registered from

Nanotechnology is one of the emerging methods in agriculture that can bring revolution to bring sustainability. Nanotechnology is not only the potential solution for agriculture but also in other allied sectors like animal husbandry, dairy, poultry, fishery etc. The use of nano-components is one of the potentials, eco-friendly and economical method by using the entire proper regulatory framework. This method should be followed properly by keeping in view of all ethical, regulatory, toxic, and policy concerns. The nanoparticles should come in to market after repeated experi-

Nanotechnology helps to developed multiple new methods for green house to field disease management as well as new area of molecular editing and manipulation for plants and pathogenic stress. Therefore diverse nanoparticles like nano-Ag, nano-Cu, nano-Zn used as potential arsenal against various destructive diseases which causes severe yield loss. One of the most important or striking feature is that considerable amount of reduction of metals compared to inorganic agrochemicals. Using of carbon nanoparticles to control disease is also under trial. Every time one thing must be remembered whenever any metal or metallic ions we are applying for nanomaterial preparation, that must be consumable through food chain additionally nontoxic and nonhazardous. It is also found that mechanism of action for each disease, different diseases on the same host and different diseases on various hosts are not the same way of treatment. Because the mode of action is different for each cases. Application of nano-globule in organic farming system is slowly upgrading and matter under consideration of developing countries agricultural policy. Some countries used copper oxychloride, copper hydroxide and copper oxide in their organic farming system in very lesser amount. Application of nanocopper in

*DOI: http://dx.doi.org/10.5772/intechopen.92463*

on agriculture.

last year's [9].

**2. Summary and future scope**

ment both in vitro and lab condition.

*Nanotechnological Approaches in Sustainable Agriculture and Plant Disease Management DOI: http://dx.doi.org/10.5772/intechopen.92463*

> improve the food quality of soybean while nano-CeO2 has reported to reduce the soybean yield and nitrogen fixing potential [69]. Therefore, the nanobased products should be in controlled use with prior precaution measures and existing regulatory frameworks to lessen the negative effects on agriculture.


#### **2. Summary and future scope**

*Organic Agriculture*

items and beverages. A sensory coating is applied on the product through wrapping or encapsulation method, which slightly modifies the color of the product by improving the taste and shelf-life of the food item. Thin layer of nanoparticles protects the food products from spoilage by holding moisture. Some of the nanoparticles proven promising in lessening the post-harvest loss are carbon nanoparticles, zinc oxide etc., which are mainly antibacterial [9].

6.**Nanotechnological applications in agriculture and animal husbandry:** In livestock management and animal husbandry, nanochips are installed in poultry and livestock to check their health and behavior through sensory technique. Quality of the poultry and livestock products can also be tested by using this method. The quality of meat or any contaminated particles present in it can be checked to avoid microbial spoilage [64]. In case of milk and milk products, **pasteurization** and improved nutrition to increase its quality are the possible ways through nanotechnological system. If nano carrier-based particles are employed in egg production, it will lessen pathogenic infection and transport

7.**Nanotechnological applications in fishery and aquaculture:** Nanotechnology is found to be one of the effective methods in aquaculture in order to increase the nutritional quality like protein and oil and also to promote the health of the fish and other sea foods [9]. Sensory-based drug delivery system was proved to be promising in detection of pathogen present in fish thereby promoting good health and quality nutrition. Nanoparticles mixed in water can also detect the presence of algae or any other micro-organisms and control eutrophication by reducing the phosphorus compounds [66]. Additionally, the encapsulated nanoparticles can effectively be used for fish feed, promoting the

8.**Waste management approaches through nanotechnological applications for sustainable agriculture:** Researchers have done enough study on sustainable management of both solid and liquid waste in such a way that it can reduce the soil and water contamination. Scientists observed that implementation of nanotechnique, for example, bionanomaterials and nanobiomimics in waste management is a promising solution. Nanofibers can effectively be used to prepare absorbable substances that can degrade the waste materials in less time without leaving any toxic substances in addition to improve the produc-

9.Concerns about nanotechnology in terms of environmental protection as well

a.**Toxicological and environmental safety concerns:** The more use of nanoparticles can lead to negativity on agriculture as many nanosubstances have proven toxic to some beneficial micro-organisms. The uncontrolled application of nanobased agrochemicals can usher to environmental pollution and disturbances in food chain. Before mass release of any nanoparticles, it should be checked properly for safety protocols of toxic composition and its residual effect [9]. A previous study has reported that the pure aluminum-based nanoparticles inhibit root growth of many plants while aluminum oxide-based nanoparticles act as a pollutant and hamper crop growth [68]. Similarly, nanozinc oxide-based products

the nutrients properly resulting quality egg production [65].

fish growth, increasing water quality etc. [67].

tion and quality of biomass [9].

as human development:

**22**

Nanotechnology is one of the emerging methods in agriculture that can bring revolution to bring sustainability. Nanotechnology is not only the potential solution for agriculture but also in other allied sectors like animal husbandry, dairy, poultry, fishery etc. The use of nano-components is one of the potentials, eco-friendly and economical method by using the entire proper regulatory framework. This method should be followed properly by keeping in view of all ethical, regulatory, toxic, and policy concerns. The nanoparticles should come in to market after repeated experiment both in vitro and lab condition.

Nanotechnology helps to developed multiple new methods for green house to field disease management as well as new area of molecular editing and manipulation for plants and pathogenic stress. Therefore diverse nanoparticles like nano-Ag, nano-Cu, nano-Zn used as potential arsenal against various destructive diseases which causes severe yield loss. One of the most important or striking feature is that considerable amount of reduction of metals compared to inorganic agrochemicals. Using of carbon nanoparticles to control disease is also under trial. Every time one thing must be remembered whenever any metal or metallic ions we are applying for nanomaterial preparation, that must be consumable through food chain additionally nontoxic and nonhazardous. It is also found that mechanism of action for each disease, different diseases on the same host and different diseases on various hosts are not the same way of treatment. Because the mode of action is different for each cases. Application of nano-globule in organic farming system is slowly upgrading and matter under consideration of developing countries agricultural policy. Some countries used copper oxychloride, copper hydroxide and copper oxide in their organic farming system in very lesser amount. Application of nanocopper in

organic farming system found to be very effective for its sustainable, environment friendly and long term approach. Researchers are trying to develop micro and macro nutrient enriched plant growth nano promoter. This type of nano-complex not only gives protection against various destructive diseases but also enhancing the plant growth with its slow leaching compatibility mode. Application of different metallic ions and carbon-nanomer under genetic delivery system is the modern topic of research. Introduction of modern tolls like quantum dots and biosensor (with combination of nanotechnology) in plant pathology can greatly replace conventional ELISA technique for plant viral detection.

The use of nanobased pesticide, herbicide and fungicide, is an attractive advancement in plant health management keeping in focus of the healthy environment. It has the ability to deliver the pesticide to the specified target with low evaporation loss, improve the solubility, overcome resistance of pesticide in insects and pests, and have better shelf-life. Due to lacking of long-term based in vivo experimental trial, observation or monitoring, more research should be carried out on the particular field. To brand this method as a successful one, material scientists and biologists should cooperate with each other to gain more deep knowledge on complex nanosystems. The reliable, efficient nanoparticles should be chosen that can be biocompatible and no harmful to the plant system. Before releasing, any nanoparticles-based products in to the market, their toxicity, impact on environment, and other risk assessment factors should be analyzed properly. This method is yet to reach to the farmers mostly in developing countries. Therefore, government should work on this to create awareness among the farmers. The journey of nanotechnology in plant disease management and different other sectors of agriculture is under progressive research and wide adaptation through farmers level. The multidisciplinary nanotechnological approaches needs support from government and government-induced schemes or policies and also support from funding agencies, which will lead to a sustainable agriculture system by lessening the global food production as a challenge for future.
