**7. Acknowledgements**

The authors are indebted to Mr. Gregory Tselepidis, for his help in preparing the paper. The financial support for this study was provided by the Alexander Technological Educational Institute of Thessaloniki and is gratefully acknowledged.

#### **8. References**


CLEMAM, Taxonomic Database on European Marine Molusca.

122 Aquaculture

We developed a DNA methodology based on PCR amplification and sequencing analysis of the mitochondrial 16S rDNA gene, in order to discriminate two different taxa of the family Pectinidae - *Flexopecten glaber* and *Flexopecten proteus* - and for a population study of both taxa. Fifty polymorphic nucleotide sites and fifty one different haplotypes were revealed in total. The majority of the genetic variation (96.98%) was distributed within populations. The levels of haplotype and nucleotide diversity were relatively high for both taxa, probably due to the large effective population sizes and the good population status. No species specific position was found and the two highly frequent haplotypes are shared between taxa, suggesting possible hybridization between them. Genetic distance between *F*. *glaber* and *F*. *proteus* was D = 0.005 and no genetic differentiation was revealed among taxa, when pooling together samples from different localities. Our results show that *F*. *glaber* and *F*. *proteus* are

The authors are indebted to Mr. Gregory Tselepidis, for his help in preparing the paper. The financial support for this study was provided by the Alexander Technological Educational

Avise, J.C. (1994). *Molecular markers, Natural history and Evolution*. Chapman & Hall, ISBN

Barucca, M.; Olmo, E; Schiaparelli, S. & Canapa, A. (2004). Molecular phylogeny of the

Canapa, A.; Marota, I.; Rollo, F. & Olmo, E. (1996). Phylogenetic analysis of Veneridae

Canapa, A.; Barucca, M.; Marinelli, A. & Olmo, E. (2000a). Molecular data from the 16S

Canapa, A.; Barucca, M.; Caputo, V.; Marinelli, A.; Cerioni, P.N.; Olmo, E.; Capriglione, T. &

Chan, T.Y. & Chu, K.H. 1996. On the different forms of *Panulirus longipes femoristriga* (von

Cho, E.S.; Joung, C.G.; Sohn, S.G.; Kim, C.D. & Han, S.J. (2007). Population genetic structure

*Biology and Evolution*, Vol.12, No.5 (July 1995), pp. 887-892

*Molecular Evolution*, Vol.50, No.1 (January 2000), pp. 93–97

bivalves. *Italian Journal of Zoology*, Vol.67, S1, pp. 127-132

species. *Journal of Natural History*, Vol.30, No.3, pp. 367-387

*Evolution*, Vol.43, No.5 (November 1996), pp. 517–522

family Pectinidae (Mollusca: Bivalvia) based on mitochondrial 16S and 12S rRNA genes. *Molecular Phylogenetics and Evolutio*n, Vol.31, No.1 (April 2004), pp. 89-95 Bertorelle, G. & Slatkin, M. (1995). The number of segregating sites in expanding human

populations, with implications for estimates of demographic parameters. *Molecular* 

(Bivalvia): comparison of molecular and palaeontological data. *Journal of Molecular* 

rRNA gene for the phylogeny of Pectinidae (Mollusca: Bivalvia). *Journal of* 

Odierna, G. (2000b). A molecular analysis of the systematics of three Antarctic

Martens, 1872) (Crustacea: Decapoda: Palinuridae), with description of a new

of the ark shell Scapharca broughtonii Schrenck from Korea, China, and Russia based on COI gene sequences. *Marine Biotechnology*, Vol.9, No.2 (March 2007), pp.

conspecific, which is in agreement with the latest classification.

Institute of Thessaloniki and is gratefully acknowledged.

9780412037818, New York

**6. Conclusion** 

**7. Acknowledgements** 

203-216

**8. References** 

(http://www.somali.asso.fr/clemam/index.php) (5/2010)


Katsares, V.; Tsiora, A.; Galinou – Mitsoudi, S. & Imsiridou, A. (2008). Genetic structure of

from mtDNA sequences. *Biologia*, Vol.63, No.3, (June 2008), pp. 412 – 417 Kimura, M. (1980). A simple method for estimating evolutionary rate of base substitution

Knowlton, N. (1993). Sibling species in the sea. *Annual Review of Ecology, Evolution and* 

Knowlton, N. (2000). Molecular genetic analyses of species boundaries in the sea.

Kocher, T.D.; Thomas, W.K.; Meyer, A.; Edwards, S.V.; Paabo, S.; Villablanca, F.X. & Wilson,

Kong, X.; Yu, Z.; Liu, Y. & Chen, L. (2003). Intraspecific genetic variation in mitochondrial

Koutsoubas, D.; Galinou-Mitsoudi, S.; Katsanevakis, S.; Leontarakis, P.; Metaxatos, A. &

Kumar, S.; Dudley, J.; Nei, M. & Tamura, K. (2008). MEGA: A biologist-centric software for

Liu, B.; Dai, J. & Yu, Z. (1998). The investigation of populations by RAPD markers on oyster,

Liu, B.; Dong, B.; Xiang, J. & Wang, Z. (2007). The phylogeny of native and exotic scallops

Livi, S.; Cordisco, C.; Damiani, C.; Romanelli, M. & Crosetti, D. (2006). Identification of

Mahidol, C.; Na-Nakorn, U.; Sukmanomon, S.; Yoosuk, W.; Taniguchi, N. & Nguyen, T.T.T.

Mahidol, C.; Na-Nakorn, U.; Sukmanomon, S.; Taniguchi, N. & Nguyen, T.T.T. 2007b.

Mathews, L.M.; Schubart, C.D.; Neigel, J.E. & Felder, D.L. (2002). Genetic, ecological and

*Journal of Shellfish Research*, Vol.26, No.1, (April 2007), pp. 25-32

Vol.16, No.2, (June 1980), pp. 111-120

*Systematics*, Vol.24, (November 1993), pp. 189-216

Vol.22, No.3, (December 2003), pp. 655-660

Vol.9, No.4, (July 2008), pp. 299-306

*Qingdao*, Vol.25, pp. 85-90

1149-1161

*Hydrobiologia*, Vol.420, No.1, (February 2000), pp. 73-90

*Sciences of USA*, Vol.86, No.16, (August 1989), pp. 6196-6200

HCMR publications, ISBN 9789609805414, Athens, Greece

the endangered species *Pinna nobilis* Linnaeus, 1758 (Mollusca: Bivalvia) inferred

through comparative studies of nucleotide sequences. *Journal of Molecular Evolution*,

A.C. (1989). Dynamics of mitochondrial DNA evolution in animals: Amplification and sequencing with conserved primers*. Proceedings of the National Academy of* 

16S ribosomal gene of Zhikong scallop *Chlamys farreri*. *Journal of Shellfish Research*,

Zenetos, A. (2007). Bivalve and gastropod mollusks of commercial interest for human consumption in the Hellenic Seas, In: *State of Hellenic Fisheries*, Papaconstantinou, C.; Zenetos, A.; Vassilopoulou, V. & Tserpes, G., (Eds.), 70-84,

evolutionary analysis of DNA and protein sequences. *Briefings in Bioinformatics*,

Crassostrea talienwhanesis. *Journal of Ocean University of Qingdao*, Vol.28, pp. 82-88

cultured in China based on 16S rDNA sequences. *Journal of Ocean University of* 

bivalve species at an early developmental stage through PCR-SSCP and sequence analysis of partial 18S rDNA. *Marine Biology*, Vol.149, No.5, (August 2006), pp.

2007a. Phylogenetic relationships among nine scallop species (Bivalvia: Pectinidae) inferred from nucleotide sequences of one mitochondrial and three nuclear regions.

Mitochondrial DNA diversity of the Asian moon scallop, *Amusium pleuronectes* (Pectinidae) in Thailand. *Marine Biotechnology*, Vol.9, No.3, (May 2007), pp. 352-359

behavioural divergence between two sibling snapping shrimp species (Crustacea: Decapoda: *Alpheus*). *Molecular Ecology*, Vol.11, No.8, (August 2002), pp. 1427-1437

