**2.1.1 Establishing a stock culture**

Seawater was filtered through GFC membrane filter after passed through UV treatment before use. A 500 ml and 1000 ml beakers were used as culture vessel. The culture was maintained at temperature between 26°C to 27°C and salinity between 22 to 26 ppt. Culture was fed daily with 1 ml of baker's yeast (0.02 g/L) (Nanton and Castell, 1998) and with mixed algal (*Isochrysis* sp*.*, *Nannochloropsis* sp*.*, *Chaetoceros* sp.) at the density of 1 x 106 cell/ml. No aeration was provided for the 500 ml and 1000 ml stock cultures. The partial replacement of seawater was done every three days and full replacements at every month by passing the cultures through a 45 µm mesh net. The trapped copepods were then transferred into new culture vessel.
