**2.3.2 Statistical analyses**

Differences in expression levels of *gapdh*, *actb*, *eef1a*, *arp, ubi* and *myhc* during the photoperiod manipulation experiment were examined by one-way ANOVA with Holm-Sidak post-hoc tests. ANOVA assumptions were checked prior to carrying out the analyses and when the data did not follow the Gaussian distribution or did not meet the equal variance requirements, a Kruskal-Wallis one-way ANOVA on ranks with Dunn's test for post-hoc comparisons was used instead. Statistical analyses were performed with the SigmaStat statistical package (Systat software, London, UK). In all cases, significance was set at *P* < 0.05.
