**Figure 5.**

*Emerging Contaminants*

**Figure 3.**

*GC chromatograms of Endosulfan compound.*

**272**

**Figure 4.**

*GC chromatograms of PAH compounds.*

The standards (EN and PAHs) were prepared in certain concentrations and readings were made in the device and the calibration graphs were plotted to calcu-

The linear regression, correlation coefficient, the detection limit (LOD) indicating the performance of the method in the method validation [14], the determination limit (LOQ ), relative standard deviation percentage (RSD) and recovery calculations of the pesticides analyzed by GC-MS were shown in **Table 4**.

For each point determined, twice samples were sampled in the months of July (sowing period) and September (harvesting period) in the periods of paddy sowing

late the amounts in the actual samples (**Figures 5** and **6**).

**2.2 Collection of samples and preparation for analysis**

*The calibration graph of Endosulfan compound.*

**Figure 6.** *The calibration graphs of PAHs compounds.*

and harvesting. In the paddy pan, selected for sampling, three points were determined to represent each of the ceilings and pan water, soil and plant samples were made from these points. Since there were no paddy in the sowing period, only in the harvesting period of soil and water "at the latest 5 days before the harvest" were done every triple sampling. The pan water, sediment samples, rice grains and rice husk samples from the paddy samples were coded with the abbreviation "w," "sd," "r," and "rh," respectively. The coordinates of the locations, code and sampling dates are given in **Table 5**.

Water samples prepared for analysis of EN and PAHs are enriched in the solid phase extraction system and prepared for analysis by GC-MS. Plant and sediment


**Table 4.** *The values of linear regression (y = ax + b), correlation coefficient (R2), LOD (μg/kg), LOQ (μg/kg), and RSD% in the pesticides.*

**275**

**Figure 7.**

methods are given in **Figure 7**

*Determination of Concentration for Some Priority Substances in Paddy Fields of Ergene River…*

**1-sd 11-r**

**2-sd 22-r**

**3-sd 33-r**

**Watering source**

River

River

**1-w** 20.07.2016 Dam Lake **11-w** 22.09.2016 Dam Lake

**11-sd**

**11-rh**

**22-sd**

**22-rh**

**33-sd**

**33-rh**

**Code Sampling date**

**22-w** 23.09.2016 Meriç River

**33-w** 21.09.2016 Ergene

**Watering source**

River

**Coordinates Sowing period Harvest period**

**2-w** 27.07.2016 Meriç

**3-w** 02.08.2016 Ergene

**Code Sampling date**

samples were enriched with modified QuEChERS method and prepared for analysis by GC-MS. The contents of the solid phase extraction and modified QuEChERS

.

*The enrichment of samples with the solid phase extraction and modified QuEChERS methods.*

*DOI: http://dx.doi.org/10.5772/intechopen.93383*

40°48 ′25.0 ″N

26°18 ′58.9 ″ E

41°32 ′19.7 ″N

26°36 ′14.4 ″ E

41°20 ′17.8 ″N

26°52 ′31.6 ″ E

**Table 5.**

*Sampling's coordinates, codes, and dates.*

#### *Emerging Contaminants*


*Determination of Concentration for Some Priority Substances in Paddy Fields of Ergene River… DOI: http://dx.doi.org/10.5772/intechopen.93383*

#### **Table 5.**

*Emerging Contaminants*

**274**

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 **Table 4.**

ACE

NAP

IcdP

BaP

BkF

BbF

CRY

BaA

PYR

FLR

ANT

PHN

FLU

ACY

ACE

NAP

EN

**Pesticides**

**Regression equation (linear range)**

305.77x − 245.32 (1–1000 μg/kg)

23,096x − 6571.3 (0.25–1000 μg/kg)

16,398x − 91,561 (1–750 μg/kg)

13,125x − 47,551 (0.5–750 μg/kg)

11,643x − 78,796 (2.5–750 μg/kg)

13,342x − 98,862 (2.5–750 μg/kg)

6895.2x + 103,892 (2.5–750 μg/kg)

9819.7x − 70,254 (2.5–750 μg/kg)

9427.9x − 42,495 (2.5–750 μg/kg)

821.31x − 1687.9 (25–750 μg/kg)

1401.4x − 36,903 (25–750 μg/kg)

512.66x − 20,563 (50–750 μg/kg)

504.19x − 23,733 (50–750 μg/kg)

280.02x + 7583.6 (100–1000 μg/kg)

136.66x − 10,109 (250–1000 μg/kg)

127.67x − 7342 (250–1000 μg/kg)

214.46x − 8091.9 (50–1000 μg/kg)

*The values of linear regression (y = ax + b), correlation coefficient (R2), LOD (μg/kg), LOQ (μg/kg), and RSD% in the pesticides.*

**R2** 0.9999 0.9984 0.9989 0.9993 0.9985 0.9978 0.9992 0.9981 0.9997 0.9995 0.9985 0.9986 0.9977 0.9999 0.9981 0.9991 0.9974

14.0

46.7

5.72

95.0

42.2

141

5.05

90.2

63.1

210

3.33

86.1

17.4 20.0

66.3

7.87

82.5

58.1

6.8

84.3

12.3

40.9

4.86

83.2

4.05 6.78

22.6

1.35

86.9

13.52

4.96

92.9

0.67

2.24

3.42

97.6

1.17 0.64

2.12

2.28

91.6

3.89

3.65

100

0.43 0.64

2.12

2.17

96.5

1.46

1.62

88.1

0.05

0.17

0.42

101

0.23

0.76

1.17

98.9

0.03

0.10

1.85

105

0.79

2.64

7.39

103

**LOD**

**LOQ**

**RSD%**

**Recovery,%**

*Sampling's coordinates, codes, and dates.*

#### **Figure 7.**

*The enrichment of samples with the solid phase extraction and modified QuEChERS methods.*

samples were enriched with modified QuEChERS method and prepared for analysis by GC-MS. The contents of the solid phase extraction and modified QuEChERS methods are given in **Figure 7**.
