**Abstract**

Environmental pollution has been a challenging phenomenon in most developing countries, due to the weak enforcement of environmental regulations. As a result, humans and animals are exposed to different environmental pollutants, which threaten their very existence. Some of the emerging pollutants of great concern are polybrominated diphenyl ethers (PBDEs) since they are categorized as probable human carcinogens and are also known to bioaccumulate in fatty tissues of animals and humans, reaching toxic levels upon continued exposure. Monitoring of these pollutants is therefore paramount as it contributes to addressing the problem of human exposure and environmental pollution. Their monitoring involves sample preparation methods followed by quantification with various detection techniques. Sample preparation methods that aim at reducing matrix interferences, enriching analytes and transfer of analytes to a desirable solvent, have evolved from conventional methods to advanced methods that facilitate the detection of these chemicals at very low concentrations. Likewise, detection techniques have advanced from chromatographic detection techniques to miniaturized systems that involve sensors. This chapter discusses PBDEs as emerging pollutants, their sources, and toxicological implications on humans, as well as advances in sample preparation methods and detection techniques in the determination of PBDEs.

**Keywords:** polybrominated diphenyl ethers, persistent organic pollutants, pollution, emerging pollutants, detection techniques

## **1. Introduction**

The preservation and conservation of the environment are of great significance for healthy living. However, efforts to conserve the environment have been futile due to escalated pollution from biogenic and anthropogenic sources, which constantly release pollutants to the environment [1]. In the recent past, increased industrial and agricultural activities have immensely contributed to the pollution of aquatic environments such as rivers and streams, which pose major detrimental environmental problems to humans [2]. It is evident that industrial development has generated a myriad of new chemicals produced and applied in daily activity, which is becoming a major concern for citizens, the research community, and authorities [3]. Among the pollutant chemicals that have been introduced into the environment are polybrominated diphenyl ethers (PBDEs). PBDEs are toxic, lipophilic, hydrophobic, and persistent artificial chemicals characterized by high physical and chemical stability [4]. They are commonly applied as flame retardants in polymer products such as electronics, plastics, textiles, and building materials [5, 6]. PBDEs have become a growing concern over the last two decades due to their ubiquity, persistence and accumulation capacity in the environment, as well as their potential risks to human health and wildlife [7, 8]. PBDEs are normally additive compounds, meaning they are not covalently bound to the polymeric products [9]. Therefore, they may leach out into the surrounding environment during their production, usage, disposal, or recycling process [10]. PBDEs can be transported away from their sources for long-ranges through aqueous and/or terrestrial environmental compartments [11, 12]. In this context, monitoring and assessment of environmental pollution by these compounds are very important.

Their determination involves a series of steps from sample pre-treatment to quantification of analytes using various detection systems. Different sample preparation strategies that range from conventional to advanced strategies have been applied for the determination of PBDEs in environmental samples. Some of the conventional sample enrichment methods include Soxhlet extraction [13, 14] and liquid-liquid extraction (LLE) [15]. More recently, ultrasound-assisted extraction (UAE) [16, 17], pressurized liquid extraction (PLE) [18, 19], microwave-assisted extraction (MAE), solid-phase extraction (SPE), and solid-phase microextraction (SPME) have exhibited successful extraction of PBDEs from environmental samples [20, 21]. The application of SPE and SPME has advanced from conventional adsorbent formats to the most improved formats which allow easy transfer of analytes from their complex matrices. This has been achieved by using novel adsorbent materials to replace conventional silica-based adsorbents which exhibit low selectivity towards targeted analytes [22]. Similarly, analytical techniques for the qualitative and quantitative determination of PBDEs have advanced from well-known gas chromatography-electron capture detection (GC-ECD) to sensorbased techniques that are more advantageous in terms of excellent selectivity, with opportunities for *in-situ* application. The following sections provide detailed information on PBDEs, advances in sample pre-treatment methods and detection techniques with a view of providing the current state-of-the-art as far as their monitoring is concerned.

#### **2. Polybrominated diphenyl ethers**

#### **2.1 The chemistry of PBDEs**

PBDEs comprise of two halogenated aromatic rings bonded by an ester bond and are classified in relation to the number and position of bromine atoms in a particular molecule [23]. They have a general molecular formula of C12H(10 - x) BrxO, where x is the number of bromine atoms in a molecule with numerical values [x = 1, 2, 3, …, 10 = m + n] (**Figure 1**). Substitution of bromine atoms can take place at 10 possible positions on the two benzene rings resulting in 209 possible congeners [24].

**209**

*Polybrominated Diphenyl Ethers (PBDEs) as Emerging Environmental Pollutants…*

Different congeners are easily identified by their corresponding IUPAC numbers ranging from 1 to 209. In this case, 2,2′,4,4′-tetrabromodiphenyl ether is BDE-47, with bromine atoms in ortho and para positions on the first and second benzene

Molecules with one to four bromine atoms are classified as low molecular mass PBDEs, whereas the ones with five to ten bromine atoms are categorized as high molecular mass PBDEs. Less brominated PBDEs are more persistent and toxic than highly brominated diphenyl ethers [25]. The substitution pattern also affects the physicochemical properties of PBDEs, whereby the solubility of PBDEs decreases significantly with an increase in bromine substitution. The aqueous solubility (SW) of low molecular mass PBDEs at room temperature ranges from 6.57 × 10−7 to 7.82 × 10−11 mol L−1 while those of high molecular mass have aqueous solubility values lower than 7.82 × 10−11 mol L−1 [26]. A wide range of PBDE congeners exhibit high lipophilic capacity and high resistance to degradation; a property that makes them bioaccumulate and magnify in biota [7]. PBDEs are also associated with high octanol-air partition coefficients (KOA) with values ranging between 9.3 and 12.0 from BDE-17 to -126, which is approximately 1 to 2 orders of magnitude greater than PCBs [27]. Therefore, PBDEs are easily transported through air from one point to another, increasing their chances of exposure to humans. Dissolved organic matter has shown a high tendency to interact with hydrophobic compounds such as PBDEs, which hinders their mobility and degradation in the environment [28]. Reported binding coefficients of PBDEs (log KDOC) towards organic matter range from 5.1 to 7.14, which implicates the high

capability of PBDEs to adsorb and partition on organic matter [29].

PBDEs were commercially produced in three technical mixtures, typically known as pentaBDE, octaBDE, and decaBDE, basing on the number of bromine atoms [10]. By early 2000, the global production of commercial PBDE formulations was approximately 67,000 tons in the ratio 1:1.98:14.8 for octa-BDE, penta-BDE, and deca-BDE respectively, of which the United States production was

**2.2 Global production and regulation of PBDEs**

*Chemical structure of 2,2′,4,4′-tetrabromodiphenyl ether (BDE 47).*

*DOI: http://dx.doi.org/10.5772/intechopen.93858*

rings, respectively (**Figure 2**).

*General structural formula of PBDEs.*

**Figure 1.**

**Figure 2.**

*Polybrominated Diphenyl Ethers (PBDEs) as Emerging Environmental Pollutants… DOI: http://dx.doi.org/10.5772/intechopen.93858*

**Figure 1.** *General structural formula of PBDEs.*

*Emerging Contaminants*

of aquatic environments such as rivers and streams, which pose major detrimental environmental problems to humans [2]. It is evident that industrial development has generated a myriad of new chemicals produced and applied in daily activity, which is becoming a major concern for citizens, the research community, and authorities [3]. Among the pollutant chemicals that have been introduced into the environment are polybrominated diphenyl ethers (PBDEs). PBDEs are toxic, lipophilic, hydrophobic, and persistent artificial chemicals characterized by high physical and chemical stability [4]. They are commonly applied as flame retardants in polymer products such as electronics, plastics, textiles, and building materials [5, 6]. PBDEs have become a growing concern over the last two decades due to their ubiquity, persistence and accumulation capacity in the environment, as well as their potential risks to human health and wildlife [7, 8]. PBDEs are normally additive compounds, meaning they are not covalently bound to the polymeric products [9]. Therefore, they may leach out into the surrounding environment during their production, usage, disposal, or recycling process [10]. PBDEs can be transported away from their sources for long-ranges through aqueous and/or terrestrial environmental compartments [11, 12]. In this context, monitoring and assessment of

environmental pollution by these compounds are very important.

Their determination involves a series of steps from sample pre-treatment to quantification of analytes using various detection systems. Different sample preparation strategies that range from conventional to advanced strategies have been applied for the determination of PBDEs in environmental samples. Some of the conventional sample enrichment methods include Soxhlet extraction [13, 14] and liquid-liquid extraction (LLE) [15]. More recently, ultrasound-assisted extraction (UAE) [16, 17], pressurized liquid extraction (PLE) [18, 19], microwave-assisted extraction (MAE), solid-phase extraction (SPE), and solid-phase microextraction (SPME) have exhibited successful extraction of PBDEs from environmental samples [20, 21]. The application of SPE and SPME has advanced from conventional adsorbent formats to the most improved formats which allow easy transfer of analytes from their complex matrices. This has been achieved by using novel adsorbent materials to replace conventional silica-based adsorbents which exhibit low selectivity towards targeted analytes [22]. Similarly, analytical techniques for the qualitative and quantitative determination of PBDEs have advanced from well-known gas chromatography-electron capture detection (GC-ECD) to sensorbased techniques that are more advantageous in terms of excellent selectivity, with opportunities for *in-situ* application. The following sections provide detailed information on PBDEs, advances in sample pre-treatment methods and detection techniques with a view of providing the current state-of-the-art as far as their

PBDEs comprise of two halogenated aromatic rings bonded by an ester bond and are classified in relation to the number and position of bromine atoms in a particular molecule [23]. They have a general molecular formula of C12H(10 - x) BrxO, where x is the number of bromine atoms in a molecule with numerical values [x = 1, 2, 3, …, 10 = m + n] (**Figure 1**). Substitution of bromine atoms can take place at 10 possible positions on the two benzene rings resulting in 209 possible

**208**

congeners [24].

monitoring is concerned.

**2.1 The chemistry of PBDEs**

**2. Polybrominated diphenyl ethers**

Different congeners are easily identified by their corresponding IUPAC numbers ranging from 1 to 209. In this case, 2,2′,4,4′-tetrabromodiphenyl ether is BDE-47, with bromine atoms in ortho and para positions on the first and second benzene rings, respectively (**Figure 2**).

**Figure 2.** *Chemical structure of 2,2′,4,4′-tetrabromodiphenyl ether (BDE 47).*

Molecules with one to four bromine atoms are classified as low molecular mass PBDEs, whereas the ones with five to ten bromine atoms are categorized as high molecular mass PBDEs. Less brominated PBDEs are more persistent and toxic than highly brominated diphenyl ethers [25]. The substitution pattern also affects the physicochemical properties of PBDEs, whereby the solubility of PBDEs decreases significantly with an increase in bromine substitution. The aqueous solubility (SW) of low molecular mass PBDEs at room temperature ranges from 6.57 × 10−7 to 7.82 × 10−11 mol L−1 while those of high molecular mass have aqueous solubility values lower than 7.82 × 10−11 mol L−1 [26]. A wide range of PBDE congeners exhibit high lipophilic capacity and high resistance to degradation; a property that makes them bioaccumulate and magnify in biota [7]. PBDEs are also associated with high octanol-air partition coefficients (KOA) with values ranging between 9.3 and 12.0 from BDE-17 to -126, which is approximately 1 to 2 orders of magnitude greater than PCBs [27]. Therefore, PBDEs are easily transported through air from one point to another, increasing their chances of exposure to humans. Dissolved organic matter has shown a high tendency to interact with hydrophobic compounds such as PBDEs, which hinders their mobility and degradation in the environment [28]. Reported binding coefficients of PBDEs (log KDOC) towards organic matter range from 5.1 to 7.14, which implicates the high capability of PBDEs to adsorb and partition on organic matter [29].

#### **2.2 Global production and regulation of PBDEs**

PBDEs were commercially produced in three technical mixtures, typically known as pentaBDE, octaBDE, and decaBDE, basing on the number of bromine atoms [10]. By early 2000, the global production of commercial PBDE formulations was approximately 67,000 tons in the ratio 1:1.98:14.8 for octa-BDE, penta-BDE, and deca-BDE respectively, of which the United States production was

approximately 50% of the global production [30]. Several governmental regulations and international environmental agencies have restricted and completely banned the use and production of some PBDE congeners [31]. In 2004, the European Union phased out the use and production of penta-BDE and octa-BDE. Consequently, in December 2004, Great Lakes Chemical Corporation, a sole manufacturer of penta-BDE and octa-BDE in North America, voluntarily phased out the production of these BDE formulations [32]. These efforts were boosted by the Stockholm Convention in 2012 when it listed commercial octa-BDE and penta-BDE among persistent organic pollutants that need to be eliminated. Despite the ban in the production of most PBDEs, they are still reported in air, soil and aquatic environments, which is attributed to their stability and subsequent release from technoecosystems, and production of deca-BDE, which still continues to be produced in some countries [33, 34].

### **2.3 PBDEs in the environment and their toxicological implications**

There are diverse pathways by which PBDEs enter the environment. Major environmental sources of PBDE pollution comprise of leakage from consumer products and industrial facilities that synthesize PBDEs or PBDE-containing products [5]. Besides, PBDEs may enter the aquatic environment from illegal disposal of obsolete electrical appliances and electronic devices flame-retarded with PBDEs or other PBDEs-containing products [7]. They can also enter the aquatic environment through raw sewage and into the surrounding air through volatilization from products containing PBDEs and toxic fumes from e-waste recycling plants [35]. Since the first discovery of PBDEs in the aquatic environment on the West coast of Sweden in 1981, several studies have reported the presence of PBDEs in the environment [36]. This is despite the strict regulatory measures imposed by some governments and international environmental agencies to phase out some PBDE congeners and subsequent reduction in the production of particular PBDEs. BDE-47, 99, 100, and 153 are the ones that are frequently investigated because they are primary components of commercial mixtures, therefore, their ratios in the environment are expected to be significantly high. Moreover, less substituted BDE congeners such as BDE-28 and 47 are more toxic and non-biodegradable, hence their investigation in the environment and biota is of great significance in the monitoring of these pollutants [37]. Soil and sediment harbour higher concentrations of PBDEs, which is attributed to the organic carbon content, which makes them a sink for most organic pollutants [38]. Elevated levels of PBDEs have since been reported in agricultural soils after the application of sewage sludge at a concentration of 21 to 690 ng g−1 dry weight (dw) [39]. From statistics, human beings spend more than 70% of their lifetime indoors, in occupational offices, homes, learning institutions, and transport vehicles, and are therefore exposed to an array of contaminants from indoor dust [40]. The highest levels of PBDEs in dust samples have been reported in major industrialized cities in China and Europe at a concentration of 397–40,236 ng g−1 and 950–54,000 ng g−1, respectively [41, 42], with comparably lower levels of 1710 ng g−1 in African regions [43]. **Table 1** presents a summary of reported PBDE levels in selected environmental matrices.

The principal route for PBDE exposure to humans was thought to be through food consumption [58]. However, inhalation of contaminated indoor and outdoor dust is also a significant pathway via which human beings may be exposed to PBDEs [46, 59]. Dermal absorption is another potential route for PBDE exposure [60]. Numerous studies have reported levels up to 160.3 ng g−1 of PBDEs in human samples, such as serum and milk. Increased application of PBDEs in electronics has significantly aroused more research work on the concentration of these pollutants

**211**

*Polybrominated Diphenyl Ethers (PBDEs) as Emerging Environmental Pollutants…*

Office dust

Kuwait Sewage sludge 52.5–377\*

*Levels of PBDEs reported in the environment and biota from different locales worldwide.*

**Country Sample matrix Concentration Reference** South Africa River water 2.60–4.83 ng L−1 [44] North America River water 0.00013–0.01 ng L−1 [45] Great Britain Indoor dust 950–54,000 ng g−1 [46]

Nigeria Indoor dust 3700–19,000 ng g−1 [47] China Indoor dust 397–40,236 ng g−1 [41] Uganda Air 0.00340–0.00984 ng m−3 [48] Kenya Soil 0.19–35.64 ng g−1 [49] China Soil 4.8–533 ng g−1 [50] China Sediment 0.03- 5.22 ng g−1 [51] China Sediment 0.13–1.98 ng g−1 [52] Sweden Sewage sludge nd-450 ng g−1 [53] Spain Sewage sludge 197–1185 ng g−1 [39]

1710 ng g−1 1520 ng g−1 [43]

ng g−1 [54]

in the blood of workers in e-waste processing plants and other exposed populations [61]. BDE 47, 153, and 209 are the most predominant congeners reported in human serum and milk [55, 62]. The toxicity of PBDEs is backed up by numerous epidemiological studies. Scientific research has linked PBDE exposure to an array of adverse health effects [63]. To mention a few, penta- and octa-BDEs at a concentration of 10,000 ng g−1 have been associated with disruption of thyroid hormone homeostasis [7]. Moreover, penta- and tetra-BDEs, within the range of 8000–18,000 ng g−1, have been reported to affect the neurodevelopment of mice [64]. Exposure to high levels of deca-BDEs is likely to cause breast cancer [7]. PBDEs have been linked to developmental neurotoxicity and hence leading to severe effects on cognitive ability, behaviour, and health of both animals and humans [65, 66]. Several studies have also linked PBDEs with adverse effects on the human reproductive system. In particular, BDE-47, BDE-153, and BDE-154 in the range of 0.2–1.6 ng g−1 have been confirmed to have negative impacts on testosterone, luteinizing hormone, and estradiol [67]. Therefore, there is a need to have robust, accurate and reproducible methods to quantify PBDEs in different environmental matrices. The sections that

USA Serum 5.0–27.9 [55] South Africa Tigerfish 5.8 [56] Uganda Breast milk 0.59–8.11 [57]

follow will discuss these aspects with a particular focus on aquatic media.

Sample pre-treatment steps such as pre-concentration and clean-up are paramount before instrumental analysis [2, 68]. These steps ensure that analytes are enriched and converted into the right form/state to achieve their detection and any matrix that may interfere with the determination of the analytes is removed [69].

**3. Sample pre-treatment methods**

*DOI: http://dx.doi.org/10.5772/intechopen.93858*

South Africa Home dust

*nd, not detected.*

*Mean concentration.*

*\**

**Table 1.**


*Polybrominated Diphenyl Ethers (PBDEs) as Emerging Environmental Pollutants… DOI: http://dx.doi.org/10.5772/intechopen.93858*

#### **Table 1.**

*Emerging Contaminants*

some countries [33, 34].

approximately 50% of the global production [30]. Several governmental regulations and international environmental agencies have restricted and completely banned the use and production of some PBDE congeners [31]. In 2004, the European Union phased out the use and production of penta-BDE and octa-BDE. Consequently, in December 2004, Great Lakes Chemical Corporation, a sole manufacturer of penta-BDE and octa-BDE in North America, voluntarily phased out the production of these BDE formulations [32]. These efforts were boosted by the Stockholm Convention in 2012 when it listed commercial octa-BDE and penta-BDE among persistent organic pollutants that need to be eliminated. Despite the ban in the production of most PBDEs, they are still reported in air, soil and aquatic environments, which is attributed to their stability and subsequent release from technoecosystems, and production of deca-BDE, which still continues to be produced in

**2.3 PBDEs in the environment and their toxicological implications**

There are diverse pathways by which PBDEs enter the environment. Major environmental sources of PBDE pollution comprise of leakage from consumer products and industrial facilities that synthesize PBDEs or PBDE-containing products [5]. Besides, PBDEs may enter the aquatic environment from illegal disposal of obsolete electrical appliances and electronic devices flame-retarded with PBDEs or other PBDEs-containing products [7]. They can also enter the aquatic environment through raw sewage and into the surrounding air through volatilization from products containing PBDEs and toxic fumes from e-waste recycling plants [35]. Since the first discovery of PBDEs in the aquatic environment on the West coast of Sweden in 1981, several studies have reported the presence of PBDEs in the environment [36]. This is despite the strict regulatory measures imposed by some governments and international environmental agencies to phase out some PBDE congeners and subsequent reduction in the production of particular PBDEs. BDE-47, 99, 100, and 153 are the ones that are frequently investigated because they are primary components of commercial mixtures, therefore, their ratios in the environment are expected to be significantly high. Moreover, less substituted BDE congeners such as BDE-28 and 47 are more toxic and non-biodegradable, hence their investigation in the environment and biota is of great significance in the monitoring of these pollutants [37]. Soil and sediment harbour higher concentrations of PBDEs, which is attributed to the organic carbon content, which makes them a sink for most organic pollutants [38]. Elevated levels of PBDEs have since been reported in agricultural soils after the application of sewage sludge at a concentration of 21 to 690 ng g−1 dry weight (dw) [39]. From statistics, human beings spend more than 70% of their lifetime indoors, in occupational offices, homes, learning institutions, and transport vehicles, and are therefore exposed to an array of contaminants from indoor dust [40]. The highest levels of PBDEs in dust samples have been reported in major industrialized cities in China and Europe at a concentration of 397–40,236 ng g−1 and 950–54,000 ng g−1, respectively [41, 42], with comparably lower levels of 1710 ng g−1 in African regions [43]. **Table 1** presents a summary of reported PBDE

The principal route for PBDE exposure to humans was thought to be through food consumption [58]. However, inhalation of contaminated indoor and outdoor dust is also a significant pathway via which human beings may be exposed to PBDEs [46, 59]. Dermal absorption is another potential route for PBDE exposure [60]. Numerous studies have reported levels up to 160.3 ng g−1 of PBDEs in human samples, such as serum and milk. Increased application of PBDEs in electronics has significantly aroused more research work on the concentration of these pollutants

**210**

levels in selected environmental matrices.

*Levels of PBDEs reported in the environment and biota from different locales worldwide.*

in the blood of workers in e-waste processing plants and other exposed populations [61]. BDE 47, 153, and 209 are the most predominant congeners reported in human serum and milk [55, 62]. The toxicity of PBDEs is backed up by numerous epidemiological studies. Scientific research has linked PBDE exposure to an array of adverse health effects [63]. To mention a few, penta- and octa-BDEs at a concentration of 10,000 ng g−1 have been associated with disruption of thyroid hormone homeostasis [7]. Moreover, penta- and tetra-BDEs, within the range of 8000–18,000 ng g−1, have been reported to affect the neurodevelopment of mice [64]. Exposure to high levels of deca-BDEs is likely to cause breast cancer [7]. PBDEs have been linked to developmental neurotoxicity and hence leading to severe effects on cognitive ability, behaviour, and health of both animals and humans [65, 66]. Several studies have also linked PBDEs with adverse effects on the human reproductive system. In particular, BDE-47, BDE-153, and BDE-154 in the range of 0.2–1.6 ng g−1 have been confirmed to have negative impacts on testosterone, luteinizing hormone, and estradiol [67]. Therefore, there is a need to have robust, accurate and reproducible methods to quantify PBDEs in different environmental matrices. The sections that follow will discuss these aspects with a particular focus on aquatic media.

## **3. Sample pre-treatment methods**

Sample pre-treatment steps such as pre-concentration and clean-up are paramount before instrumental analysis [2, 68]. These steps ensure that analytes are enriched and converted into the right form/state to achieve their detection and any matrix that may interfere with the determination of the analytes is removed [69].

The choice of sample pre-treatment step is dependent on the physicochemical properties of the targeted analytes, their concentration in the environment, and the complexity of matrix interference [70, 71]. Soxhlet extraction, a traditional liquid-solid extraction method, has been used for decades in the extraction of analytes from their complex solid matrices. With the combination of polar and nonpolar solvents, the Soxhlet extraction strategy has been proved to be efficacious, achieving extraction efficiencies greater than 70% [72, 73]. However, this method is hindered by several factors such as long extraction duration, excessive solvent consumption, and the need for subsequent clean-up steps [74]. With increasing demand for economical and fast sample extraction strategies with high enrichment factors, coupled with SPE clean-up procedures, techniques such as UAE, PLE, MAE, and supercritical fluid extraction (SFE) have been adopted in enrichment of analytes from solid matrices.

UAE encompasses the introduction of a finely divided sample contained in a sample holder in an ultrasonic bath with solvent and subjected to ultrasonic radiation. UAE is a vital technique in achieving sustainable green chemistry and is primarily employed in the extraction of analytes from solid sample matrices [75, 76]. This technique can achieve complete extraction with high reproducibility within a short duration. Moreover, small quantities of extraction solvents are used as compared to conventional Soxhlet extraction [77]. Methanol, acetonitrile, ethanol, and acetone are typical extractants used in this method in minimal volume. UAE based on ultrasound assisted-dispersive solid phase extraction (UAE-DSPE) coupled to GC-MS has been reported to achieve exemplary limits of detection and extraction efficiencies for 7 BDE congeners from dust samples collected from air conditioning filters in the range of 1.4–8.4 ng g−1 and 90–102%, respectively [78]. Some of the benefits of UAE include faster kinetics and an increase in extraction yield. Ultrasound can also reduce the operating temperature allowing the extraction of thermally labile compounds [79].

Unlike traditional Soxhlet extraction that consumes a large volume of solvent, PLE, also referred to as pressurized solvent extraction, has been of great interest due to its extraction effectiveness. Extraction of analytes from their environmental matrices is achieved via a synergistic mechanism that proceeds through liquid solvents at elevated temperature and pressure, which altogether enhance extraction throughput as compared with other techniques performed at ordinary atmospheric conditions [80]. PLE is viewed as another 'green' option for traditional sample extraction methods. High temperature accomplishes a higher dispersion rate, while high pressure keeps the extraction solvent below its boiling point. During the determination of brominated flame retardants in e-waste samples, PLE and UAE were evaluated in regard to extraction efficiencies. PLE demonstrated high extraction efficiencies of 95–100% as compared to 10–50% for UAE [81]. When contrasted with the conventional methods, PLE shows a decrease in extraction time and a significant decrease in the overall consumption of organic solvents [82].

Another type of extraction technique that enables a three-fold reduction in extraction time and solvent is MAE. This is a sample extraction method that employs microwave energy to extract analytes from solid sample matrices in contact with extraction solvents. Microwave energy directly generates heat which initiates molecular motion of the analytes in the solid-solvent complex mixture, hence facilitating the mass transfer of the target analyte from the solid matrix to the extracting solvent [83, 84]. MAE has been reported to achieve good recoveries of 80–106%, 72.4–108.4%, and 80–110% in the extraction/pre-concentration of PBDEs from airborne particulate matter [85], e-waste materials [86], and sewage sludge samples [87], respectively. Compared with Soxhlet extraction, MAE achieves better recoveries and uses small amounts of solvents (30 mL versus 200 mL for

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*Polybrominated Diphenyl Ethers (PBDEs) as Emerging Environmental Pollutants…*

required, which subsequently consume extra organic solvents.

other techniques that use a large volume of solvents.

steps in the determination of PBDEs [96, 102].

While SPE continues to be used because of its affordability and ease of use, other formats that offer high enrichment factors and shorter extraction times, such as SPME, stir-bar sorptive extraction (SBSE) and dispersive solid-phase extraction (DSPE), have been introduced [103]. SPME is an innovation and improvement of conventional SPE. Its stationary phase comprises of fused-silica fibers coated with a

Soxhlet extraction), at the same time allowing control of extraction parameters, such as extraction time and temperature [88]. However, MAE has some shortcomings, whereby the extracted sample usually contains some matrix interferences, such as lipids and lipophilic compounds, therefore, filtration and clean-up steps are

Supercritical fluid extraction (SFE) is another method employed to extract PBDEs from solid matrices. Supercritical CO2 is often used as an extracting solvent, which has the capability of attaining recoveries above 97%. Moreover, the extraction efficiency of SFE can be further improved by the use of modifiers such as acetonitrile, toluene, and tetrahydrofuran [89]. A successful application of SFE in the extraction of PBDEs from polymeric materials was reported by Peng et al. [90]. The authors used supercritical CO2 as a solvent and SFE operating parameters such as temperature and pressure were optimized at 65°C and 20 MPa, respectively, achieving 97.6% extraction efficiency. This technique is a greener alternative to

Numerous methodologies have been adopted in the determination of PBDE pollutants in liquid matrices. SPE and conventional LLE have been embraced as routine extraction techniques for PBDEs in liquid samples. The extractive capability of LLE is based on the transfer of analytes from an aqueous polar phase to a non-polar organic phase [91]. LLE coupled with GC-MS has been applied in the determination of 13 PBDEs and their metabolites in water, with recoveries of 77%-102% [92]. LLE has also been a desirable extraction method in the preparation of biota samples for the determination of PBDEs. Recently, a study aimed at assessing *in utero* exposure of 24 tri- to deca- BDE congeners on primiparous mothers in Kampala, Uganda reported a successful application of LLE, with appreciable recoveries of 81–91% [93]. However, LLE has some shortcomings; it suffers from low recovery, poor selectivity, high matrix interference in chromatographic analysis and increased sample loads [94]. In addition, the extraction of PBDEs from water samples requires extremely large volumes of solvents due to their hydrophobic character and low concentration in water, thus limiting its applications [95]. To overcome these challenges, different configurations of SPE have been adopted in sample enrichment strategies. SPE is a modern sample pre-treatment technique employed to concentrate analytes from liquid samples and to remove matrix interferents during the clean-up step, achieving exemplary recoveries and reproducible results over LLE [96, 97]. SPE protocols are usually performed by the use of a small column or separation cartridge packed with an appropriate sorbent material [98, 99]. Target analytes are adsorbed by the sorbent materials and later eluted with a solvent that has a greater affinity for the analytes. The chemistry behind this separation is based on intermolecular forces between the analytes, active sites of the adsorbent, and the liquid phase of the matrix [100]. SPE can be performed through an on-line or off-line approach. The on-line SPE configuration, which may enable automation, is directly coupled with specific analytical systems such as gas chromatography (GC) or high-performance liquid chromatography (HPLC). Whereas in the off-line protocol, a pre-concentration step is done separately using cartridges and further eluting the adsorbed analyte with an appropriate solvent for eventual chromatographic analysis [101]. Because of its robustness and flexibility, SPE has been widely employed in different analytical procedures in pre-concentration and clean-up

*DOI: http://dx.doi.org/10.5772/intechopen.93858*

#### *Polybrominated Diphenyl Ethers (PBDEs) as Emerging Environmental Pollutants… DOI: http://dx.doi.org/10.5772/intechopen.93858*

Soxhlet extraction), at the same time allowing control of extraction parameters, such as extraction time and temperature [88]. However, MAE has some shortcomings, whereby the extracted sample usually contains some matrix interferences, such as lipids and lipophilic compounds, therefore, filtration and clean-up steps are required, which subsequently consume extra organic solvents.

Supercritical fluid extraction (SFE) is another method employed to extract PBDEs from solid matrices. Supercritical CO2 is often used as an extracting solvent, which has the capability of attaining recoveries above 97%. Moreover, the extraction efficiency of SFE can be further improved by the use of modifiers such as acetonitrile, toluene, and tetrahydrofuran [89]. A successful application of SFE in the extraction of PBDEs from polymeric materials was reported by Peng et al. [90]. The authors used supercritical CO2 as a solvent and SFE operating parameters such as temperature and pressure were optimized at 65°C and 20 MPa, respectively, achieving 97.6% extraction efficiency. This technique is a greener alternative to other techniques that use a large volume of solvents.

Numerous methodologies have been adopted in the determination of PBDE pollutants in liquid matrices. SPE and conventional LLE have been embraced as routine extraction techniques for PBDEs in liquid samples. The extractive capability of LLE is based on the transfer of analytes from an aqueous polar phase to a non-polar organic phase [91]. LLE coupled with GC-MS has been applied in the determination of 13 PBDEs and their metabolites in water, with recoveries of 77%-102% [92]. LLE has also been a desirable extraction method in the preparation of biota samples for the determination of PBDEs. Recently, a study aimed at assessing *in utero* exposure of 24 tri- to deca- BDE congeners on primiparous mothers in Kampala, Uganda reported a successful application of LLE, with appreciable recoveries of 81–91% [93]. However, LLE has some shortcomings; it suffers from low recovery, poor selectivity, high matrix interference in chromatographic analysis and increased sample loads [94]. In addition, the extraction of PBDEs from water samples requires extremely large volumes of solvents due to their hydrophobic character and low concentration in water, thus limiting its applications [95]. To overcome these challenges, different configurations of SPE have been adopted in sample enrichment strategies. SPE is a modern sample pre-treatment technique employed to concentrate analytes from liquid samples and to remove matrix interferents during the clean-up step, achieving exemplary recoveries and reproducible results over LLE [96, 97]. SPE protocols are usually performed by the use of a small column or separation cartridge packed with an appropriate sorbent material [98, 99]. Target analytes are adsorbed by the sorbent materials and later eluted with a solvent that has a greater affinity for the analytes. The chemistry behind this separation is based on intermolecular forces between the analytes, active sites of the adsorbent, and the liquid phase of the matrix [100]. SPE can be performed through an on-line or off-line approach. The on-line SPE configuration, which may enable automation, is directly coupled with specific analytical systems such as gas chromatography (GC) or high-performance liquid chromatography (HPLC). Whereas in the off-line protocol, a pre-concentration step is done separately using cartridges and further eluting the adsorbed analyte with an appropriate solvent for eventual chromatographic analysis [101]. Because of its robustness and flexibility, SPE has been widely employed in different analytical procedures in pre-concentration and clean-up steps in the determination of PBDEs [96, 102].

While SPE continues to be used because of its affordability and ease of use, other formats that offer high enrichment factors and shorter extraction times, such as SPME, stir-bar sorptive extraction (SBSE) and dispersive solid-phase extraction (DSPE), have been introduced [103]. SPME is an innovation and improvement of conventional SPE. Its stationary phase comprises of fused-silica fibers coated with a

*Emerging Contaminants*

analytes from solid matrices.

of thermally labile compounds [79].

The choice of sample pre-treatment step is dependent on the physicochemical properties of the targeted analytes, their concentration in the environment, and the complexity of matrix interference [70, 71]. Soxhlet extraction, a traditional liquid-solid extraction method, has been used for decades in the extraction of analytes from their complex solid matrices. With the combination of polar and nonpolar solvents, the Soxhlet extraction strategy has been proved to be efficacious, achieving extraction efficiencies greater than 70% [72, 73]. However, this method is hindered by several factors such as long extraction duration, excessive solvent consumption, and the need for subsequent clean-up steps [74]. With increasing demand for economical and fast sample extraction strategies with high enrichment factors, coupled with SPE clean-up procedures, techniques such as UAE, PLE, MAE, and supercritical fluid extraction (SFE) have been adopted in enrichment of

UAE encompasses the introduction of a finely divided sample contained in a sample holder in an ultrasonic bath with solvent and subjected to ultrasonic radiation. UAE is a vital technique in achieving sustainable green chemistry and is primarily employed in the extraction of analytes from solid sample matrices [75, 76]. This technique can achieve complete extraction with high reproducibility within a short duration. Moreover, small quantities of extraction solvents are used as compared to conventional Soxhlet extraction [77]. Methanol, acetonitrile, ethanol, and acetone are typical extractants used in this method in minimal volume. UAE based on ultrasound assisted-dispersive solid phase extraction (UAE-DSPE) coupled to GC-MS has been reported to achieve exemplary limits of detection and extraction efficiencies for 7 BDE congeners from dust samples collected from air conditioning filters in the range of 1.4–8.4 ng g−1 and 90–102%, respectively [78]. Some of the benefits of UAE include faster kinetics and an increase in extraction yield. Ultrasound can also reduce the operating temperature allowing the extraction

Unlike traditional Soxhlet extraction that consumes a large volume of solvent, PLE, also referred to as pressurized solvent extraction, has been of great interest due to its extraction effectiveness. Extraction of analytes from their environmental matrices is achieved via a synergistic mechanism that proceeds through liquid solvents at elevated temperature and pressure, which altogether enhance extraction throughput as compared with other techniques performed at ordinary atmospheric conditions [80]. PLE is viewed as another 'green' option for traditional sample extraction methods. High temperature accomplishes a higher dispersion rate, while high pressure keeps the extraction solvent below its boiling point. During the determination of brominated flame retardants in e-waste samples, PLE and UAE were evaluated in regard to extraction efficiencies. PLE demonstrated high extraction efficiencies of 95–100% as compared to 10–50% for UAE [81]. When contrasted with the conventional methods, PLE shows a decrease in extraction time and a significant decrease in the overall consumption of organic solvents [82].

Another type of extraction technique that enables a three-fold reduction in extraction time and solvent is MAE. This is a sample extraction method that employs microwave energy to extract analytes from solid sample matrices in contact with extraction solvents. Microwave energy directly generates heat which initiates molecular motion of the analytes in the solid-solvent complex mixture, hence facilitating the mass transfer of the target analyte from the solid matrix to the extracting solvent [83, 84]. MAE has been reported to achieve good recoveries of 80–106%, 72.4–108.4%, and 80–110% in the extraction/pre-concentration of PBDEs from airborne particulate matter [85], e-waste materials [86], and sewage sludge samples [87], respectively. Compared with Soxhlet extraction, MAE achieves better recoveries and uses small amounts of solvents (30 mL versus 200 mL for

**212**

polydimethylsiloxane (PDMS) layer which are reusable. With this new formulation, the application of SPE has become versatile such that it can accommodate small volumes of samples. Furthermore, SPME has been considered an almost solventfree extraction technique and can be easily automated as compared to conventional SPE [104, 105]. A miniaturized SPME has been applied in the extraction of PBDEs in environmental water samples followed by GC-MS quantitation, with low limits of detection and appreciable recoveries of 76.5–125.4% [106]. SBSE is a similar technique to SPME that has been adopted in the enrichment of PBDEs in liquid samples due to its improved extraction efficiency. The stir bars are coated with a thinner PDMS layer, as opposed to a thicker layer in SPME, a factor that allows improved enrichment efficiency [107, 108]. DSPE is another format of SPE based on the dispersion of solid sorbent materials in liquid samples to facilitate the isolation and extraction of target analytes from the complex sample matrix. In this process, matrix interferences remain embedded in the supernatant, which is later discarded while the target analyte is bound to the sorbent material and which is eventually eluted with a viable solvent [109]. DSPE has been employed in the enrichment and determination of PBDEs with recoveries within the range of 60–140% [110].

#### **3.1 Advances in SPE sorbents**

Complexity and matrix interferences encountered during sample preparation steps have attracted the invention of more selective sorbents to replace conventional silica sorbents that are associated with a number of drawbacks, such as instability at extreme pHs and low extraction efficiencies [111]. The new sorbents that include, nanocomposite materials, metal-organic frameworks, and molecularly imprinted polymers, among others, are characterized with high sensitivity and selectivity towards various environmental organic pollutants. They achieve fast dispersion and efficient recycling when applied in complex sample matrices [112, 113]. Reported nanocomposite sorbents in SPE for PBDE-containing samples include carbon nanotubes, graphene oxide (GO) [114, 115], and magnetic nanocomposite materials [113]. However, nanocomposite sorbents in classical SPE schemes have been associated with various drawbacks. A few of these challenges have been described in flow as well as batch systems, which originate from a slow flow rate of the sample through the packed SPE column and difficulty in separating the sorbent from the large volume of aqueous sample [113].

Other sorbent materials with fascinating properties are metal-organic frameworks (MOFs). These are hybrids of organic and inorganic materials characterized by a porous structure, large surface area, uniform nanoscale cavities, high adsorption capacity, and high thermal and chemical stability. Due to these advantageous properties, this class of materials has recently attracted enormous attention in the field of sample preparation [116]. The development of MOF adsorbents is still at its infancy stages, therefore, a limited number of studies have reported their application particularly in enrichment and determination of environmental PBDEs. A zirconium-based metal-organic framework material (UiO-66-OH) is a good example of a MOF. It has been synthesized and successfully applied as an adsorbent in SPME for enrichment and detection of 5 BDE congeners in milk samples using GC-MS, with low limits of detection in the range of 0.15–0.35 ng L−1 and excellent recoveries of 74.7%–118.0% [117]. A contrast study using silica-based sorbents in SPE for determination of 12 PBDEs in human serum, achieved mean recoveries of 64–95% and limits of detection in the range of 0.1–4.0 ng g−1 by using GC-MS [102], an evidence that MOF sorbents offer promising analytical results as compared with conventional sorbents.

With growing interest in sorbents that offer extraordinary extractive capability in SPE, molecularly imprinted polymers (MIPs) have been extensively explored

**215**

**Table 2.**

*Polybrominated Diphenyl Ethers (PBDEs) as Emerging Environmental Pollutants…*

as attractive options due to their robustness and selectivity towards particular target analytes providing exemplary substitute sorbents in sample clean-up and pre-concentration steps, especially in SPE and SPME [118]. MIPs are synthesized through molecular imprinting technology that involves polymerizing functional and cross-linking monomers in the presence of a target analyte, followed by the removal of the analyte to leave behind analyte-specific cavities. Their selectivity enables substantive removal of matrix interferents during the sample pre-treatment step [119]. MIP-based sorbents are readily available substitutes to silica-based adsorbents, which are reported to suffer from matrix interference, low selectivity, and sensitivity towards organic pollutants and may involve multiple steps that are labour-intensive for complete removal of interferences [120]. For example, commercial molecularly imprinted solid-phase extraction (MISPE) cartridges alongside alkaline extraction have been applied in aqueous enrichment and quantitation of PBDEs using GC-MS [121]. The extraction of PBDEs using MISPE gave recoveries above 60% compared to alkaline extraction which was below 60%. This confirms the selectivity capability of MIPs towards PBDEs from a complex environmental matrix. A more recent study has also reported recoveries of 60–87% in clean-up of soil and sediment samples using dummy molecularly imprinted polymers as SPE sorbent materials during determina-

*DOI: http://dx.doi.org/10.5772/intechopen.93858*

tion of BDE-47 and BDE-99 [122].

SPE BDE-28, 47, 49,

PLE BDE-28, 47, 99,

LLE BDE-17, 28, 47,

UAE BDE-1, 3, 7, 8,

SPME BDE-49, 99, 100,

MAE BDE-47, 99, 100,

*Examples of sample preparation strategies.*

66, 85, 99, 100, 138, 153, 154, 183 & 209

100, 153, 154 & 183

BDE-28, 47, 99, 100, 154, 155 & 183

66, 71, 85, 99, 100, 138, 153, 154, 183 & 190

> 42 mono- to deca-BDEs

> > 28 & 47

153 & 154

138, 153, 154, 184 & 209

**PBDE congeners Sample** 

BDE-47 and 99 Soil and

**analyzed**

Human serum

bottom sediment

Soil and sediment

Soil and sediment

Indoor dust sample

Industrial effluent

Milk and water

Sewage sludge

**Analytical technique**

**% Recoveries**

GC-ECD 64–95 [102]

GC-MS 60–87 [125]

GC-MS 84–103 [92]

GC-MS 85–103 [92]

GC-MS ≥ 70 [72]

HPLC 98.7 [126]

GC-ECD 90–119 [127]

GC-MS 80–110 [87]

Soil GC-MS 95 ± 9 [68]

**Reference**

**Sample preparation technique**

Soxhlet extraction *Polybrominated Diphenyl Ethers (PBDEs) as Emerging Environmental Pollutants… DOI: http://dx.doi.org/10.5772/intechopen.93858*

as attractive options due to their robustness and selectivity towards particular target analytes providing exemplary substitute sorbents in sample clean-up and pre-concentration steps, especially in SPE and SPME [118]. MIPs are synthesized through molecular imprinting technology that involves polymerizing functional and cross-linking monomers in the presence of a target analyte, followed by the removal of the analyte to leave behind analyte-specific cavities. Their selectivity enables substantive removal of matrix interferents during the sample pre-treatment step [119]. MIP-based sorbents are readily available substitutes to silica-based adsorbents, which are reported to suffer from matrix interference, low selectivity, and sensitivity towards organic pollutants and may involve multiple steps that are labour-intensive for complete removal of interferences [120]. For example, commercial molecularly imprinted solid-phase extraction (MISPE) cartridges alongside alkaline extraction have been applied in aqueous enrichment and quantitation of PBDEs using GC-MS [121]. The extraction of PBDEs using MISPE gave recoveries above 60% compared to alkaline extraction which was below 60%. This confirms the selectivity capability of MIPs towards PBDEs from a complex environmental matrix. A more recent study has also reported recoveries of 60–87% in clean-up of soil and sediment samples using dummy molecularly imprinted polymers as SPE sorbent materials during determination of BDE-47 and BDE-99 [122].


**Table 2.**

*Examples of sample preparation strategies.*

*Emerging Contaminants*

**3.1 Advances in SPE sorbents**

large volume of aqueous sample [113].

polydimethylsiloxane (PDMS) layer which are reusable. With this new formulation, the application of SPE has become versatile such that it can accommodate small volumes of samples. Furthermore, SPME has been considered an almost solventfree extraction technique and can be easily automated as compared to conventional SPE [104, 105]. A miniaturized SPME has been applied in the extraction of PBDEs in environmental water samples followed by GC-MS quantitation, with low limits of detection and appreciable recoveries of 76.5–125.4% [106]. SBSE is a similar technique to SPME that has been adopted in the enrichment of PBDEs in liquid samples due to its improved extraction efficiency. The stir bars are coated with a thinner PDMS layer, as opposed to a thicker layer in SPME, a factor that allows improved enrichment efficiency [107, 108]. DSPE is another format of SPE based on the dispersion of solid sorbent materials in liquid samples to facilitate the isolation and extraction of target analytes from the complex sample matrix. In this process, matrix interferences remain embedded in the supernatant, which is later discarded while the target analyte is bound to the sorbent material and which is eventually eluted with a viable solvent [109]. DSPE has been employed in the enrichment and determination of PBDEs with recoveries within the range of 60–140% [110].

Complexity and matrix interferences encountered during sample preparation steps have attracted the invention of more selective sorbents to replace conventional silica sorbents that are associated with a number of drawbacks, such as instability at extreme pHs and low extraction efficiencies [111]. The new sorbents that include, nanocomposite materials, metal-organic frameworks, and molecularly imprinted polymers, among others, are characterized with high sensitivity and selectivity towards various environmental organic pollutants. They achieve fast dispersion and efficient recycling when applied in complex sample matrices [112, 113]. Reported nanocomposite sorbents in SPE for PBDE-containing samples include carbon nanotubes, graphene oxide (GO) [114, 115], and magnetic nanocomposite materials [113]. However, nanocomposite sorbents in classical SPE schemes have been associated with various drawbacks. A few of these challenges have been described in flow as well as batch systems, which originate from a slow flow rate of the sample through the packed SPE column and difficulty in separating the sorbent from the

Other sorbent materials with fascinating properties are metal-organic frameworks

(MOFs). These are hybrids of organic and inorganic materials characterized by a porous structure, large surface area, uniform nanoscale cavities, high adsorption capacity, and high thermal and chemical stability. Due to these advantageous properties, this class of materials has recently attracted enormous attention in the field of sample preparation [116]. The development of MOF adsorbents is still at its infancy stages, therefore, a limited number of studies have reported their application particularly in enrichment and determination of environmental PBDEs. A zirconium-based metal-organic framework material (UiO-66-OH) is a good example of a MOF. It has been synthesized and successfully applied as an adsorbent in SPME for enrichment and detection of 5 BDE congeners in milk samples using GC-MS, with low limits of detection in the range of 0.15–0.35 ng L−1 and excellent recoveries of 74.7%–118.0% [117]. A contrast study using silica-based sorbents in SPE for determination of 12 PBDEs in human serum, achieved mean recoveries of 64–95% and limits of detection in the range of 0.1–4.0 ng g−1 by using GC-MS [102], an evidence that MOF sorbents

offer promising analytical results as compared with conventional sorbents.

With growing interest in sorbents that offer extraordinary extractive capability in SPE, molecularly imprinted polymers (MIPs) have been extensively explored

**214**

However, a wide range of limitations still exist in MIPs, especially their poor water compatibility. Consequently, since MIPs and target analytes mainly interact through hydrogen-bonding, their recognition capability would be easily disturbed by polar solvents such as water. Therefore, the adsorption process is normally performed in non-polar or low-polar solvents such as dichloromethane and *n*-hexane rather than polar solvents. Additionally, polar solvents have a tendency to occupy binding sites, which affects the recognition capacity for the target analytes. In this context, it is necessary to continually invent new synthesis strategies for watercompatible MIPs [123, 124]. A summary of some of the sample pre-concentation strategies and their extraction efficiencies is presented in **Table 2**.
