**2. Materials and methods**

*Emerging Contaminants*

and endocrine disrupting effects [4–6]. EN residues in nature are also known to remain in the soil for at least 6 years [7, 8]. Therefore, it requires examination of the soil contaminated with EN, the product grown in soil, sediment and the water used. Polycyclic systems occur when one ring is sharing two carbons with another ring, or the rings are connected to each other by a C▬C bond [9]. PAHs are from the group of compounds which show unsaturation in molecular formulas and do not give addition reactions which are characteristic for them. In the cyclic structure, PAHs from the class of planar molecules are resistant to oxidation. In addition, PAHs can be found in petrochemical, rubber, plastic, mineral oil, rust oil, paint, leather and other products. Rubber and plastic materials are high-risk materials containing PAH. In the Ergene Basin, where the industry is intense, these compounds are likely to be found. As the molecular weights of PAHs increase, their solubility in water decreases. However, their toxic and carcinogenic properties increase [9–12]. Contaminated soil, air and aquatic products may also contain PAH. The cooking meat or other food on the grill or at high temperatures increases the amount of PAH in food [11]. In this study, analysis of EN and PAHs compounds shown in **Figure 1** was performed.

The organic components (PAHs and EN) we analyzed are considered among the primary pollutants [9, 13–17]. When the pesticides in our ecological environment are taken in high concentration, they can cause deformations on the biological structure of the organism. In the event of prolonged exposure to certain pesticides, cancer can be seen or short-term exposure may result in direct death [13, 15–19]. The extensively used high sensitivity analytical techniques for the determination of EN and PAHs at low concentrations in environmental samples are

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**Figure 1.**

*Structural formulas of analyzed compounds.*

Within the scope of this study, the paddy production areas in the agricultural areas of Thrace Region, direct irrigation from Ergene-Meriç rivers and dam ponds, Ergene and Meriç Basin were evaluated and three regions were determined as the study area. The sampling points are shown in **Figure 2**. Endosulfan and PAH analyses were made in paddy plant, sediment and irrigation water samples taken from paddy fields, which irrigated from the Ergene River, the Meriç River, and the Yenikarpuzlu Dam Reservoir, considering the distinction of irrigation resources in the rice plants often grown in river basins in the Thrace region. In the Yenikarpuzlu village of Edirne province, around Sığırcı Dam, and from the irrigated area from Edirne-İpsala-Yenikarpuzlu, Edirne-Merkez-Üyüklütatar and Edirne-Uzunköprü-Muhacirkadı Village were collected the paddy, sediment and paddy irrigation water (**Figure 2**). Endosulfan and PAH analysis were performed method validation by GC-MS.

Agilent GC-MS was used in the determination phase for all studies. The instrument used is the HP-5 MS UI capillary column (30 m × 250μm × 0.25 μm) and the 5990C (Agilent) inert MSD mass detector with 7890A (Agilent) model GC-MS. The electron ionization (EI) system with 70 eV ionization energy in GC-MS, and the He gas was used as carrier gas.

**Figure 2.** *Representation of the sampling points on the map.*
